Identification and light-dependent translocation of a cone-specific antigen, cone arrestin, recognized by monoclonal antibody 7G6

PURPOSE: To elucidate the antigen recognized by monoclonal antibody (mAb) 7G6, a widely used cone-specific marker. METHODS: 7G6 immunocytochemistry was performed on sections of human, primate, and bovine retina. The antigen was immunoprecipitated from human retinal lysates and purified with protein G. Edman degradation and liquid chromatography of tryptic peptides combined with tandem mass spectrometry (LC-MS/MS) identified the antigen. RESULTS: Sequencing of peptides derived from the immunoprecipitated 7G6 antigen identified it as cone arrestin. The identity was confirmed by Western blot analysis with recombinant human cone arrestin and competition with the antibody in immunocytochemistry. Subcellular localization of cone arrestin in dark-adapted and bleached bovine retinas showed that cone arrestin accumulated in cone outer segments of light-adapted retina but was more concentrated in the inner segments of dark-adapted retina. By expression of truncated human cone arrestin mutants systematically deleting areas divergent from bovine and primate cone arrestins, the epitope of 7G6 was identified as a divergent loop exposed at the surface within the N-domain of cone arrestin. CONCLUSIONS: Several independent methods established that the 7G6 antigen is cone arrestin. The 7G6 epitope is contained in a divergent loop, the sequence of which is conserved in bovine and primates but not other vertebrate species consistent with the specificity of the antibody. The light-dependent translocation of cone arrestin suggests a role in light-dark adaptation of cones. Because of the location of its gene on the X-chromosome, cone arrestin is a candidate gene for X-linked cone dystrophies.Supported by NIH Grant R01EY08123 (WB). Additional support came from the Macular Vision Research Foundation, Research to Prevent Blindness, Inc., and a Center grant from the Foundation Fighting Blindness to the University of Utah. WB is the recipient of a Senior Investigator Award from RPB and a Ralph and Mary Tuck endowment to the Department of Ophthalmology at the University of Utah

Twenty years, eight legs, one concept: Describing spider biodiversity in Zootaxa (Arachnida: Araneae)

Zootaxa published more than a thousand papers on Araneae from 2002 to the present, including descriptions of 3,833 new spider species and 177 new genera. Here we summarise the key contributions of Zootaxa to our current knowledge of global spider diversity. We provide a historical account of the researchers that have actively participated as editors, and recognize the more than 1,000 reviewers without whom none of this would have been possible. We conduct a simple analysis of the contributions by authors and geographic region, which allows us to uncover some of the underlying trends in current spider taxonomy. In addition, we examine some of the milestones in twenty years of spider systematic research in Zootaxa. Finally, we discuss future prospects of spider taxonomy and the role that Zootaxa and its younger sister journal Megataxa will play in it. We would like to dedicate this contribution to the memory of Norman I. Platnick, a crucial figure in the advancement of spider systematics.Fil: Jäger, Peter. Senckenberg Research Institute; AlemaniaFil: Arnedo, Miquel. Universidad de Barcelona; EspañaFil: Fernandes de Azevedo, Guilherme Henrique. San Diego State University; Estados UnidosFil: Baehr, Barbara. Queensland Museum; AustraliaFil: Bonaldo, Alexandre B.. Museu Paraense Emílio Goeldi; BrasilFil: Haddad, Charles R.. University of the Free State; SudáfricaFil: Harms, Danilo. Universitat Hamburg; AlemaniaFil: Hormiga, Gustavo. The George Washington University; Estados UnidosFil: Labarque, Facundo Martín. Universidade Federal do São Carlos; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Muster, Christoph. No especifíca;Fil: Ramirez, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Santos, Adalberto J.. Universidade Federal de Minas Gerais; Brasi

Hak - hak asasi manusia dalam politik luar negeri

x, 230 hlm,; 21 x 14 c

hak-hak asasi manusia dalam Politik Luar Negeri

x.231 hal.;21 c

Revolusi, Militerisasi dan Konsolidasi Pembangunan

21 cm.; xiv; 428 hlm