TIMP3 interplays with apelin to regulate cardiovascular metabolism in hypercholesterolemic mice

Tissue inhibitor of metalloproteinase 3 (TIMP3) is an extracellular matrix (ECM) bound protein, which has been shown to be downregulated in human subjects and experimental models with cardiometabolic disorders, including type 2 diabetes mellitus, hypertension and atherosclerosis. The aim of this study was to investigate the effects of TIMP3 on cardiac energy homeostasis during increased metabolic stress conditions

Sustainable Community Gardens Require Social Engagement and Training: A Users\u2019 Needs Analysis in Europe

Urban gardens are spreading in many cities across Europe, with community gardening being a fundamental form of urban agriculture. While the literature reveals the essential role that community gardens can play in terms of learning and education, no studies have investigated the training needs for participants in community gardens to ensure their successful development. The goal of this article is to evaluate the training requirements of urban community gardens to ensure their successful implementation and their contribution to sustainability in European cities. Two questionnaires of users\u2019 needs analysis were designed and implemented in Berlin, Bologna, Budapest, and Cartagena. The results unveiled the need to re-enforce the training in the formation and community building phases of community gardens towards ensuring the creation of an engaged gardening community to maintain activity, particularly for top-down activities (e.g., research-related gardens). Users claimed their need for being trained on crop management skills (e.g., maintenance, bed preparation, organic practices) and on communication skills to further disseminate their activity, thereby increasing the potential for citizen engagement. Such requirements could be overcome with the creation of urban gardens networks, where experiences and knowledge are shared among practitioners. Policy recommendations are provided based on the outputs of this study

DNA Microarrays for Identifying Fishes

In many cases marine organisms and especially their diverse developmental stages are difficult to identify by morphological characters. DNA-based identification methods offer an analytically powerful addition or even an alternative. In this study, a DNA microarray has been developed to be able to investigate its potential as a tool for the identification of fish species from European seas based on mitochondrial 16S rDNA sequences. Eleven commercially important fish species were selected for a first prototype. Oligonucleotide probes were designed based on the 16S rDNA sequences obtained from 230 individuals of 27 fish species. In addition, more than 1200 sequences of 380 species served as sequence background against which the specificity of the probes was tested in silico. Single target hybridisations with Cy5-labelled, PCR-amplified 16S rDNA fragments from each of the 11 species on microarrays containing the complete set of probes confirmed their suitability. True-positive, fluorescence signals obtained were at least one order of magnitude stronger than false-positive cross-hybridisations. Single nontarget hybridisations resulted in cross-hybridisation signals at approximately 27% of the cases tested, but all of them were at least one order of magnitude lower than true-positive signals. This study demonstrates that the 16S rDNA gene is suitable for designing oligonucleotide probes, which can be used to differentiate 11 fish species. These data are a solid basis for the second step to create a “Fish Chip” for approximately 50 fish species relevant in marine environmental and fisheries research, as well as control of fisheries products

Oral hypoglycemic agents and the heart failure conundrum: Lessons from and for outcome trials

Type 2 diabetes is not only an independent risk factor for cardiovascular (CV) disease but is also associated with a greater incidence of heart failure (HF). The aim of this review is to examine the effects of oral antidiabetic drugs on CV disease and HF

Posttranslational modulation of FoxO1 contributes to cardiac remodeling in post-ischemic heart failure

Forkhead box protein O1 (FoxO1) plays a key role in energy homeostasis, stress response and autophagy and is dysregulated in diabetes and ischemia. We investigated cardiac FoxO1 expression and posttranstranslational modifications after myocardial infarction (MI) and further tested if active posttranstranslational modulation of FoxO1 can alter cardiac remodeling in postischemic heart failure

2-hydroxycaproate predicts cardiovascular mortality in patients with atherosclerotic disease

Background and aims: We aimed to identify novel biomarkers for cardiovascular mortality through a non-targeted metabolomics approach in patients with established atherosclerotic disease from the Tor Vergata Atherosclerosis Registry (TVAR). Methods: We compared the serum baseline metabolome of 19 patients with atherosclerosis suffering from cardiovascular death during follow-up with the baseline serum metabolome of 20 control patients matched for age, gender, body mass index (BMI) and atherosclerotic disease status, who survived during the observation period. Results: Three metabolites were significantly different in the cardiovascular mortality (CVM) group compared to controls: 2-hydroxycaproate, gluconate and sorbitol. 2-hydroxycaproate (otherwise known as alpha hydroxy caproate) was also significantly correlated with time to death. The metabolites performed better when combined together rather than singularly on the identification of CVM status. Conclusions: Our analysis led to identify few metabolites potentially amenable of translation into the clinical practice as biomarkers for specific metabolic changes in the cardiovascular system in patients with established atherosclerotic disease