Ending Laminations and Cannon-Thurston Maps

In earlier work, we had shown that Cannon-Thurston maps exist for Kleinian surface groups. In this paper we prove that pre-images of points are precisely end-points of leaves of the ending lamination whenever the Cannon-Thurston map is not one-to-one. In particular, the Cannon-Thurston map is finite-to-one. This completes the proof of the conjectural picture of Cannon-Thurston maps for surface groups.Comment: v4: Final version 22pgs 2figures. Includes the main theorem of the appendix arXiv:1002.2090 by Shubhabrata Das and Mahan Mj. To appear in Geometric and Functional Analysi

Room temperature ferromagnetism in chemically synthesized ZnO rods

We report structural and magnetic properties of pure ZnO rods using X-ray diffraction (XRD), magnetization hysteresis (M-H) loop and near edge x-ray fine structure spectroscopy (NEXAFS) study at O K edge. Sample of ZnO was prepared by co-precipitation method. XRD and selective area electron diffraction measurements infer that ZnO rods exhibit a single phase polycrystalline nature with wurtzite lattice. Field emission transmission electron microscopy, field emission scanning electron microscopy micrographs infers that ZnO have rod type microstructures with dimension 200 nm in diameter and 550 nm in length. M-H loop studies performed at room temperature display room temperature ferromagnetism in ZnO rods. NEXAFS study reflects absence of the oxygen vacancies in pure ZnO rods.Comment: 8 Pages, 3 Figure

A Combination Theorem for Metric Bundles

We define metric bundles/metric graph bundles which provide a purely topological/coarse-geometric generalization of the notion of trees of metric spaces a la Bestvina-Feighn in the special case that the inclusions of the edge spaces into the vertex spaces are uniform coarsely surjective quasi-isometries. We prove the existence of quasi-isometric sections in this generality. Then we prove a combination theorem for metric (graph) bundles (including exact sequences of groups) that establishes sufficient conditions, particularly flaring, under which the metric bundles are hyperbolic. We use this to give examples of surface bundles over hyperbolic disks, whose universal cover is Gromov-hyperbolic. We also show that in typical situations, flaring is also a necessary condition.Comment: v3: Major revision: 56 pages 5 figures. Many details added. Characterization of convex cocompact subgroups of mapping class groups of surfaces with punctures in terms of relative hyperbolicity given v4: Final version incorporating referee comments: 63 pages 5 figures. To appear in Geom. Funct. Ana

The pCS20 PCR assay for <i>Ehrlichia ruminantium<i/> does not cross-react with the novel deer ehrlichial agent found in white-tailed deer in the United States of America

White-tailed deer are susceptible to heartwater (Ehrlichia [Cowdria] ruminantium infection) and are likely to suffer high mortality if the disease spreads to the United States. It is vital, therefore, to validate a highly specific and sensitive detection method for E. ruminantium infection that can be reliably used in testing white-tailed deer, which are reservoirs of antigenically or genetically related agents such as Ehrlichia chaffeensis, Anaplasma (Ehrlichia) phagocytophilum (HGE agent) and Ehrlichia ewingii. Recently, a novel but as yet unnamed ehrlichial species, the white-tailed deer ehrlichia (WTDE), has been discovered in deer populations in the United States. Although the significance of WTDE as a pathogen is unknown at present, it can be distinguished from other Ehrlichia spp. based on 16S rRNA gene sequence analysis. In this study it was differentiated from E. ruminantium by the use of the pCS20 PCR assay which has high specificity and sensitivity for the detection of E. ruminantium. This assay did not amplify DNA from the WTDE DNA samples isolated from deer resident in Florida, Georgia and Missouri, but amplified the specific 279 bp fragment from E. ruminantium DNA. The specificity of the pCS20 PCR assay for E. ruminantium was confirmed by Southern hybridization. Similarly, the 16S PCR primers (nested) that amplify a specific 405-412 bp fragment from the WTDE DNA samples, did not amplify any product from E. ruminantium DNA. This result demonstrates that it would be possible to differentiate between E. ruminantium and the novel WTDE agent found in white tailed deer by applying the two respective PCR assays followed by Southern hybridizations. Since the pCS20 PCR assay also does not amplify any DNA products from E. chaffeensis or Ehrlichia canis DNA, it is therefore the method of choice for the detection of E. ruminantium in these deer and other animal hosts

Comparing the detection of exposure to Ehrlichia ruminantium infection on a heartwater-endemic farm by the pCS20 polymerase chain reaction assay and an indirect MAP1-B enzyme linked immunosorbent assay

Detection of heartwater is not always easy especially because all the serological assays so far available either have poor sensitivity or specificity. The indirect MAP-1B ELISA has been reported to be the most specific test for heartwater, although it does also detect antibodies to some closely related ehrlichial agents. This study was undertaken to compare two methods for the detection of heartwater infection caused by the ehrlichial agent Ehrlichia (Cowdria) ruminantium. Fifteen cattle on a heartwater-endemic farm infested with high numbers of Amblyomma hebraeum ticks, and hence exposure to E. ruminantium infection were monitored over an 8-week period by pCS20 PCR and an indirect MAP-1B ELISA. Infection was detected by pCS20 PCR in most animals with the highest number of positives (60%) in week 6 of the study. Similarly, exposure to E. ruminantium was detected by indirect MAP-1B ELISA in some animals, with the highest number of seropositives (27%) at weeks 2 - 6 of the study. The data demonstrated a fluctuating rickettsaemia in cattle in a heartwater-endemic area. Comparison of the two tests indicated that the pCS20 PCR assay was more reliable because it detected more infections than the indirect MAP-1B ELISA and would therefore be the method of choice for detection of E. ruminantium infection.The articles have been scanned with a HP Scanjet 8300; 600dpi, saved in TIFF format. Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.United States Agency for International Development (USAID).mn201