Effects of pre- and postnatal exposure to garlic and aniseed flavour on pre- and postweaning feed intake in pigs

The low nutrient intake shortly after weaning is a major cause of post-weaning problems. Feed intake after weaning is strongly related to feed intake during lactation. Feed intake during lactation, however, varies considerably between litters. We hypothesised that prenatal and postnatal exposure to certain flavours would increase the intake of feed containing the same flavours pre- and postweaning. Multiparous sows did (n = 17) or did not (n = 14) receive 50 g garlic granulate/powder and 25 g aniseed as daily additive to their diet during the last month of gestation and during lactation. From day 14 of lactation, litters were submitted to intermittent suckling: 12 h separation form the sow each day. During lactation, all litters had 40 g garlic and 20 g aniseed per kg added to their creep feed. After weaning, half of the litters had no additive in their diet. Piglets were weaned at 4 weeks (13 litters) or at 6 weeks (18 litters). At 6 weeks of lactation, litters of which the dam received the flavour in her diet, had a higher feed intake (309 ± 43 vs 233 ± 35 g/p/d) than litters of dams without the flavour, although the difference was not significant. Sow diet had no effect on postweaning feed intake, but postweaning piglet diet did. Late (week 6) weaned litters receiving the flavoured feed had a higher feed intake from 3 to 10 days after weaning (833 ± 38 vs 687 ± 58 g/p/d). Weight gain during the first 10 days after weaning was not affected by sow or pig diets. Feed intake and weight gain shortly after weaning were strongly related to feed intake during lactation (overall R = 0.64, P <0.05 and R = 0.77, P <0.05). We conclude that early experience with flavours increases later acceptance and improves adaptation to post weaning conditions

Cystic ovaries in intermittently-suckled sows: follicle growth and endocrine profiles

This paper presents follicle development and hormone profiles for sows with normal ovulation or cystic follicles during an intermittent-suckling (IS) regime that started at Day 14 of lactation. Sows were subjected to separation from their piglets during blocks of 6 h or 12 h. In total, 8 out of 52 sows developed cystic follicles; either full cystic ovaries (n = 6) or partial ovulation (n = 2). Increase in follicle size of these sows was similar to that of normal ovulating sows until pre-ovulatory size at Day 5 after the start of separation, but from then on became larger (P <0.05). LH surge was smaller or absent in sows that developed (partially) cystic ovaries (0.4 ± 0.1 vs 3.6 ± 0.3 ng mL–1; P <0.01). Peak levels of oestradiol (E2) were similar but high E2 levels persisted in sows that developed (partly) cystic ovaries and duration of oestrus tended to be longer. The risk of developing (partly) cystic ovaries was higher when IS occurred in blocks of 6 h versus 12 h (33 vs 10%). In conclusion, the appearance of cystic ovaries at approximately Day 20 of ongoing lactation was related to an insufficient LH surge, as is also the case in non-lactating sows

Effect of excessive, hormonally induced intrauterine crowding in the gilt on fetal development on day 40 of pregnancy

Selection for litter size may result in an increase in uterine crowding due to a faster increase in ovulation rate than in litter size. Increased ovulation rate does not result in a proportionally increased number of piglets born alive. In this study, the effect of ovulation rate on vitality characteristics of fetal-placental units at d 40 of pregnancy was investigated. For this, 43 Large White gilts were treated with hormones to induce superovulation. Average ovulation rate was 45.16 ± 13.22; average number of vital fetuses at d 40 of pregnancy was 17.09 ± 3.61 that weighed 11.26 ± 1.99 g; their placenta weighed 31.88 ± 14.79 g; and they occupied 11.69 ± 4.90 cm of the uterus. Loss in oocytes (i.e., that did not result in a vital fetus at d 40) increased with increasing ovulation rate and occurred before (early mortality; P = 0.0003) and after implantation (late mortality, i.e., traces visible at d 40; P <0.0001). With respect to the vital fetuses, increased ovulation rate resulted in decreased fetal (P = 0.0008) and placental weight (P = 0.0008) and decreased length of the area in the uterus that was occupied by the placenta (P = 0.0011). Strong correlations existed between placental and fetal weight [0.68; 95% confidence interval (CI) = 0.64 to 0.72], and placental weight and length (0.78; 95% CI = 0.74 to 0.82). Fetal-placental characteristics were weakly correlated to distance to the implantation sites of neighboring fetuses, a measure of crowdedness [-0.002 (95% CI = -0.042 to 0.038) with fetal weight to 0.16 (95% CI = 0.12 to 0.20) with placental length]. Increased ovulation rates, but more specifically increased late mortality rates, have negative effects on the remaining vital fetuses with respect to the fetal (P = 0.0085) and placental weight (P <0.0001) and length of the implantation site (P = 0.0016). The most extreme effect was on placental weight, in which a uterus with 18 cases of late mortality (P <0.0001). Furthermore, increased ovulation rates resulted in decreased within litter variation for fetal (P = 0.0018) and placental weight (P = 0.0084). At increased ovulation rates, the number of live fetuses remained similar, but placental development is impaired and the growth of the fetus is retarded compared with reduced ovulation rate, with effects likely lasting into adult life

Caudal vena cava progesterone and LH release patterns on Day 14 of gestation in primiparous sows

The aim of the present study was to explore the relationship between systemic and local progesterone secretion and LH pulsatility during implantation in the pig. Differences in progesterone concentrations measured locally in the caudal vena cava and systemically in the jugular vein were studied in eight primiparous sows on Day 14 of pregnancy. LH pulsatility was analysed for its effects on the local progesterone-releasing pattern. Mean (± s.d.) progesterone concentrations in the vena cava (65.5 ± 19.8 ng mL–1) were approximately double basal concentrations (33.6 ± 13.1 ng mL–1). Basal concentrations of progesterone and LH were calculated as the average of the lowest six values. Basal caudal vena cava and mean jugular (27.6 ± 1.5 ng mL–1) progesterone concentrations did not differ significantly. Pre- and postprandial jugular progesterone concentrations were significantly different in the morning and afternoon (P = 0.025 and 0.023). Mean LH ranged from 0.24 to 0.43 ng mL–1 and was approximately double as high as basal LH in individual sows. In 60.8% of cases, LH pulses were followed by a progesterone pulse within 1 h. In conclusion, the present study showed that corpus luteum function appears to respond to LH pulsatility on Day 14 of pregnancy. However, the response varies at the level of individual sows. In addition, systemic postprandial decreases in progesterone were confirmed on Day 14 of pregnancy

Associations of conformation and locomotive characteristics in growing gilts with osteochondrosis at slaughter

Osteochondrosis (OC) and abnormalities in conformation and locomotive characteristics (CLC) have been associated with premature culling in sows. Several CLC have been suggested to be associated with OC and might help as an in vivo indicator for and increased risk of having OC. The aim of this study was to investigate associations of OC with CLC assessed at several ages in growing gilts from 2 separate experiments over the effects of dietary restriction (Exp. 1) and floor type (Exp. 2) on OC prevalence. In Exp. 1, gilts (n = 211) were subjectively assessed for CLC at, on average, 4, 9, 11, 16, and 24 wk of age. In Exp. 2, gilts (n = 212) were subjectively assessed for CLC at, on average, 4, 9, 11, 16, and 22 wk of age. Assessment was done on 10 conformation and 2 locomotive characteristics using a 9-point grading scale by 2 observers. At, on average, 27 wk of age in Exp. 1 and 24 wk of age in Exp. 2, gilts were slaughtered and the knee, elbow, and hock joints were macroscopically assessed for OC. The CLC most frequently associated with OC were O shape or X shape of the hind legs, straight or bowed hind legs, and straight or sickled hock. X-shaped hind legs were associated with OC at slaughter in the knee joint at 4, 9, and 24 wk of age and at the animal level (all joints taken together) at 4, 9, and 16 wk of age. Straight or bowed hind legs were associated with OC at slaughter in the knee joint at 4 and 11 wk of age; in the hock joint at 11 wk of age; and at the animal level at 4, 9, 11, and 22 wk of age. Straight or sickled hock was associated with OC at slaughter in the knee joint at 4 wk of age, in the hock joint at 9 and 22 wk of age, and at the animal level at 9 and 22 wk of age. Results show that several CLC assessed at several ages were associated with OC, but consistent associations of a type of CLC in every assessment could not be found. The associations of CLC with OC are, therefore, difficult to be used as an in vivo indicator of increased risk for OC

Feeding level does not affect progesterone levels in intermittently suckled sows with lactational ovulation

The aim of this study was to examine whether the low post-ovulatory plasma P4 levels found in intermittently suckled (IS) sows are related to the high feeding level during intermittent lactation. Multiparous sows (n = 21) were separated from their piglets for 12 h per day starting at day 14 of lactation until 6 days after ovulation. At day 28 of lactation, 9 sows had ovulated (spontaneous) and the remaining 12 sows were treated with PG600 (PG600-induced). At ovulation sows were allocated to either a high (H) feeding level (on average 6.5 kg) or a low (L) feeding level (high feeding level minus 2.5 kg) until 6 days after ovulation. Plasma P4 levels were not affected by feeding level or type of ovulation (P > 0.10), and neither were CL parameters, embryo survival rate and embryo development at day 30 of gestation. In conclusion, low levels of plasma P4 were found in IS sows with lactational ovulation but these were not affected by feeding level during the first week after ovulation. Further studies are needed to investigate which factors cause the lower plasma P4 levels in these sows

Validation of transrectal ultrasonography for assessment of corpora lutea characteristics in pregnant sows and its relationship with litter characteristics at birth

In experiment 1 we investigated the accuracy of transrectal ultrasonography (TUS) to assess the number (OR) and diameter of corpora lutea (CL) in 45 and 25 sows, respectively, at 23.4 ± 2.9 d of pregnancy. The diameter was calculated as the average diameter of 10 biggest CL. Sows were subsequently slaughtered and OR was assessed by dissection of CL from both ovaries (n = 45) and average diameter of the 10 biggest CL was also calculated after measurement of CL with the caliper rule (n = 25). There was a weak relationship between OR counted after dissection of the ovaries and OR counted with TUS (β = 0.28 ± 0.01 CL/CL, P = 0.01), but there was a strong relationship between the average CL diameter measured with the caliper rule after dissection and the average CL diameter based on TUS (β = 1.0 ± 0.1 mm/mm, P < 0.0001). This shows that TUS is not a valid method to assess OR in pregnant sows but it is a valid method to assess average CL diameter. In experiment 2, we investigated the relationship between the average CL diameter assessed by TUS (n = 100) at 23.8 ± 2.4 d of pregnancy and average piglet birth weight (BW) and observed an increase of 37.6 ± 17.8 g in piglet BW per mm increase in average CL diameter measured by TUS (P = 0.04). This relationship is probably because larger CL develop from bigger follicles at ovulation, which might have ovulated oocytes of higher quality that developed into embryos with higher growth potential and thus higher birth weight

Insulin-stimulating diets during the weaning-to-estrus interval do not improve fetal and placental development and uniformity in high-prolific multiparous sows

Piglet birth weight and litter uniformity are important for piglet survival. Insulin-stimulating sow diets before mating may improve subsequent piglet birth weights and litter uniformity, but the physiological mechanisms involved are not clear. This study evaluated effects of different levels of insulin-stimulating feed components (dextrose plus starch; fed twice daily) during the weaning-to-estrus interval (WEI) on plasma insulin and IGF-1 concentrations, and on follicle development and subsequent luteal, fetal and placental development and uniformity at days 42 to 43 of pregnancy. During WEI, multiparous sows were isocalorically fed diets supplemented with 375 g/day dextrose plus 375 g/day corn starch (INS-H), with 172 g/day dextrose plus 172 g/day corn starch and 144 g/day animal fat (INS-L), or with 263 g/day animal fat (CON). Jugular vein catheters were inserted through the ear vein at 1.5 days before weaning to asses plasma insulin and IGF-1 concentrations. After estrus, all sows received a standard gestation diet until slaughter at days 42 to 43 of pregnancy. The dextrose plus starch-diets enhanced the postprandial insulin response in a dose-dependent manner (e.g. at day 2 insulin area under the curve was 4516 µU/444 min for CON, 8197 µU/444 min for INS-L and 10 894 µU/444 min for INS-H; s.e.m. = 694; P <0.001), but did not affect plasma IGF-1 concentrations during the first 3 days of WEI. Follicle development and subsequent luteal, fetal and placental development and uniformity were not affected by the dietary treatments, nor related to plasma insulin and IGF-1 concentrations during WEI. Pre-weaning plasma insulin and IGF-1 concentrations were negatively related to sow body condition loss during lactation, but were not related to subsequent reproduction characteristics. This study shows that dietary dextrose plus starch are effective in stimulating insulin secretion (both postprandial peak and long-term concentration), but not IGF-1 secretion during the first 3 days after weaning in multiparous sows. The extreme insulin-stimulating diets during WEI did, however, not improve follicle development, or subsequent development and uniformity of fetuses and placentas in these high-prolific sows (27.0 ± 0.6 ovulations; 18.6 ± 0.6 vital fetuses