Restoring the sting to metric preheating

The relative growth of field and metric perturbations during preheating is sensitive to initial conditions set in the preceding inflationary phase. Recent work suggests this may protect super-Hubble metric perturbations from resonant amplification during preheating. We show that this possibility is fragile and sensitive to the specific form of the interactions between the inflaton and other fields. The suppression is naturally absent in two classes of preheating in which either (1) the vacua of the non-inflaton fields during inflation are deformed away from the origin, or (2) the effective masses of non-inflaton fields during inflation are small but during preheating are large. Unlike the simple toy model of a $g^2 \phi^2 \chi^2$ coupling, most realistic particle physics models contain these other features. Moreover, they generically lead to both adiabatic and isocurvature modes and non-Gaussian scars on super-Hubble scales. Large-scale coherent magnetic fields may also appear naturally.Comment: 6 pages, 3 ps figures, RevTex, revised discussion of backreaction and new figure. To appear Phys. Rev. D (Rapid Communication

Overlap Distribution of the Three-Dimensional Ising Model

We study the Parisi overlap probability density P_L(q) for the three-dimensional Ising ferromagnet by means of Monte Carlo (MC) simulations. At the critical point P_L(q) is peaked around q=0 in contrast with the double peaked magnetic probability density. We give particular attention to the tails of the overlap distribution at the critical point, which we control over up to 500 orders of magnitude by using the multi-overlap MC algorithm. Below the critical temperature interface tension estimates from the overlap probability density are given and their approach to the infinite volume limit appears to be smoother than for estimates from the magnetization.Comment: 7 pages, RevTex, 9 Postscript figure

Chagas disease reactivation in a heart transplant patient infected by domestic Trypanosoma cruzi discrete typing unit I (TcIDOM)

Background Trypanosoma cruzi, causative agent of Chagas disease, displays high intraspecific genetic diversity: six genetic lineages or discrete typing units (DTUs) are currently recognized, termed TcI through TcVI. Each DTU presents a particular distribution pattern across the Americas, and is loosely associated with different transmission cycles and hosts. Several DTUs are known to circulate in Central America. It has been previously suggested that TcI infection is benign and does not lead to chronic chagasic cardiomyopathy (CCC). Findings In this study, we genotyped T. cruzi parasites circulating in the blood and from explanted cardiac tissue of an El Salvadorian patient who developed reactivation Chagas disease while on immunosuppressive medications after undergoing heart transplant in the U.S. as treatment for end-stage CCC. Parasite typing was performed through molecular methods (restriction fragment length polymorphism of polymerase reaction chain amplified products, microsatellite typing, maxicircle sequence typing and low-stringency single primer PCR, [LSSP-PCR]) as well as lineage-specific serology. We show that the parasites infecting the patient belong to the TcI DTU exclusively. Our data indicate that the parasites isolated from the patient belong to a genotype frequently associated with human infection throughout the Americas (TcI DOM ). Conclusions Our results constitute compelling evidence in support of TcI DTU’s ability to cause end-stage CCC and help dispel any residual bias that infection with this lineage is benign, pointing to the need for increased surveillance for dissemination of this genotype in endemic regions, the USA and globally

Photosynthesis and conductance of spring-wheat leaves: field response to continuous free-air atmospheric CO2 enrichment

Spring wheat was grown from emergence to grain maturity in two partial pressures of CO2 (pCO2): ambient air of nominally 37 Pa and air enriched with CO2 to 55 Pa using a free-air CO2 enrichment (FACE) apparatus. This experiment was the first of its kind to be conducted within a cereal field without the modifications or disturbance of microclimate and rooting environment that accompanied previous studies. It provided a unique opportunity to examine the hypothesis that continuous exposure of wheat to elevated pCO2 will lead to acclimatory loss of photosynthetic capacity. The diurnal courses of photosynthesis and conductance for upper canopy leaves were followed throughout the development of the crop and compared to model-predicted rates of photosynthesis. The seasonal average of midday photosynthesis rates was 28% greater in plants exposed to elevated pCO2 than in contols and the seasonal average of the daily integrals of photosynthesis was 21% greater in elevated pCO2 than in ambient air. The mean conductance at midday was reduced by 36%. The observed enhancement of photosynthesis in elevated pCO2 agreed closely with that predicted from a mechanistic biochemical model that assumed no acclimation of photosynthetic capacity. Measured values fell below predicted only in the flag leaves in the mid afternoon before the onset of grain-filling and over the whole diurnal course at the end of grain-filling. The loss of enhancement at this final stage was attributed to the earlier senescence of flag leaves in elevated pCO2. In contrast to some controlled-environment and field-enclosure studies, this field-scale study of wheat using free-air CO2 enrichment found little evidence of acclimatory loss of photosynthetic capacity with growth in elevated pCO2 and a significant and substantial increase in leaf photosynthesis throughout the life of the crop

Does Leaf Position within a Canopy Affect Acclimation of Photosynthesis to Elevated CO2? . Analysis of a Wheat Crop under Free-Air CO2 Enrichment

Previous studies of photosynthetic acclimation to elevated CO2 have focused on the most recently expanded, sunlit leaves in the canopy. We examined acclimation in a vertical profile of leaves through a canopy of wheat (Triticum aestivum L.). The crop was grown at an elevated CO2 partial pressure of 55 Pa within a replicated field experiment using free-air CO2 enrichment. Gas exchange was used to estimate in vivo carboxylation capacity and the maximum rate of ribulose-1,5-bisphosphate-limited photosynthesis. Net photosynthetic CO2 uptake was measured for leaves in situ within the canopy. Leaf contents of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), light-harvesting-complex (LHC) proteins, and total N were determined. Elevated CO2 did not affect carboxylation capacity in the most recently expanded leaves but led to a decrease in lower, shaded leaves during grain development. Despite this acclimation, in situ photosynthetic CO2 uptake remained higher under elevated CO2. Acclimation at elevated CO2 was accompanied by decreases in both Rubisco and total leaf N contents and an increase in LHC content. Elevated CO2 led to a larger increase in LHC/Rubisco in lower canopy leaves than in the uppermost leaf. Acclimation of leaf photosynthesis to elevated CO2 therefore depended on both vertical position within the canopy and the developmental stage

A multi-scale modelling framework combining musculoskeletal rigid-body simulations with adaptive finite element analyses, to evaluate the impact of femoral geometry on hip joint contact forces and femoral bone growth

Multi-scale simulations, combining muscle and joint contact force (JCF) from musculoskeletal simulations with adaptive mechanobiological finite element analysis, allow to estimate musculoskeletal loading and predict femoral growth in children. Generic linearly scaled musculoskeletal models are commonly used. This approach, however, neglects subject- and age-specific musculoskeletal geometry, e.g. femoral neck-shaft angle (NSA) and anteversion angle (AVA). This study aimed to evaluate the impact of proximal femoral geometry, i.e. altered NSA and AVA, on hip JCF and femoral growth simulations. Musculoskeletal models with NSA ranging from 120° to 150° and AVA ranging from 20° to 50° were created and used to calculate muscle and hip JCF based on the gait analysis data of a typically developing child. A finite element model of a paediatric femur was created from magnetic resonance images. The finite element model was morphed to the geometries of the different musculoskeletal models and used for mechanobiological finite element analysis to predict femoral growth trends. Our findings showed that hip JCF increase with increasing NSA and AVA. Furthermore, the orientation of the hip JCF followed the orientation of the femoral neck axis. Consequently, the osteogenic index, which is a function of cartilage stresses and defines the growth rate, barely changed with altered NSA and AVA. Nevertheless, growth predictions were sensitive to the femoral geometry due to changes in the predicted growth directions. Altered NSA had a bigger impact on the growth results than altered AVA. Growth simulations based on mechanobiological principles were in agreement with reported changes in paediatric populations

Gemini Observations of Disks and Jets in Young Stellar Objects and in Active Galaxies

We present first results from the Near-infrared Integral Field Spectrograph (NIFS) located at Gemini North. For the active galaxies Cygnus A and Perseus A we observe rotationally-supported accretion disks and adduce the existence of massive central black holes and estimate their masses. In Cygnus A we also see remarkable high-excitation ionization cones dominated by photoionization from the central engine. In the T-Tauri stars HV Tau C and DG Tau we see highly-collimated bipolar outflows in the [Fe II] 1.644 micron line, surrounded by a slower molecular bipolar outflow seen in the H_2 lines, in accordance with the model advocated by Pyo et al. (2002).Comment: Invited paper presented at the 5th Stromlo Symposium. 9 pages, 7 figures. Accepted for publication in Astrophysics & Space Scienc

Genetic determinants of cellular addiction to DNA polymerase theta

Polymerase theta (Pol θ, gene name Polq) is a widely conserved DNA polymerase that mediates a microhomology-mediated, error-prone, double strand break (DSB) repair pathway, referred to as Theta Mediated End Joining (TMEJ). Cells with homologous recombination deficiency are reliant on TMEJ for DSB repair. It is unknown whether deficiencies in other components of the DNA damage response (DDR) also result in Pol θ addiction. Here we use a CRISPR genetic screen to uncover 140 Polq synthetic lethal (PolqSL) genes, the majority of which were previously unknown. Functional analyses indicate that Pol θ/TMEJ addiction is associated with increased levels of replication-associated DSBs, regardless of the initial source of damage. We further demonstrate that approximately 30% of TCGA breast cancers have genetic alterations in PolqSL genes and exhibit genomic scars of Pol θ/TMEJ hyperactivity, thereby substantially expanding the subset of human cancers for which Pol θ inhibition represents a promising therapeutic strategy

SO(10) unified models and soft leptogenesis

Motivated by the fact that, in some realistic models combining SO(10) GUTs and flavour symmetries, it is not possible to achieve the required baryon asymmetry through the CP asymmetry generated in the decay of right-handed neutrinos, we take a fresh look on how deep this connection is in SO(10). The common characteristics of these models are that they use the see-saw with right-handed neutrinos, predict a normal hierarchy of masses for the neutrinos observed in oscillating experiments and in the basis where the right-handed Majorana mass is diagonal, the charged lepton mixings are tiny. In addition these models link the up-quark Yukawa matrix to the neutrino Yukawa matrix Y^\nu with the special feature of Y^\nu_{11}-> 0 Using this condition, we find that the required baryon asymmetry of the Universe can be explained by the soft leptogenesis using the soft B parameter of the second lightest right-handed neutrino whose mass turns out to be around 10^8 GeV. It is pointed out that a natural way to do so is to use no-scale supergravity where the value of B ~1 GeV is set through gauge-loop corrections.Comment: 26 pages, 2 figures. Added references, new appendix of a relevant fit and improved comment