RPG Educacional Utilizando o Conceito de Agentes

Este artigo apresenta o desenvolvimento do RPGEDU no que serefere à utilização de agentes inteligentes para seleção das atividades (ou tarefas) que o “jogador” realizará, demonstra o que é um RPG e o que é um agente inteligente. Demonstra o que é um RPG e inteligência do agente no RPGEDU

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Uma metodologia para o projeto teórico de conversores moleculares de luz A methodology for the theoretical design of light conversion molecular devices

<abstract language="eng">Recentlly, we have proposed the representation of lanthanides within AM1 as sparkles for the purpose of obtaing ground state geometries of their complexes. We tested our quantum chemical sparkle model (SMLC/AM1) for the prediction of the crystallographic structure of complexes with coordination number nine, eight and seven. A technique is introduced for the theoretical prediction of eletronic spectra of the organic part of lanthanide complexes by replacing the metal ion by a point charge with the ligands held in their positions as determined by the SMLC/AM1, and by computing the theoretical spectra via the intermediate neglect of differential overlap/spectroscopic-configuration interaction (INDO/S-CI)

Caracterização de geoambientes da floresta nacional do purus, Amazônia ocidental: uma contribuição ao plano de manejo Geoenvironments of the purus national forest: a contribution to the management plan

A falta de estudos básicos necessários para o plano de manejo vem retardando a implementação efetiva de diversas Unidades de Conservação na região amazônica. Neste trabalho, interpretações de imagens orbitais e semiorbitais aliadas a trabalhos de campo foram utilizadas para a caracterização geoambiental da Floresta Nacional (FLONA) do Purus. Através da geração de mapas temáticos, criou-se uma base digital georreferenciada que constitui a primeira plataforma para o SIG da FLONA. Foram identificados quatro geoambientes principais: Platôs Dissecados com Mata sobre Latossolos e Argissolos; Encostas e Rampas com Mata sobre Argissolos; Planícies Aluviais Eutróficas com Neossolos Flúvicos e Gleissolos; e Planícies Aluviais Distróficas com Gleissolos. Os aluviões holocênicos na área sob influência do rio Purus são ricos em nutrientes (eutróficos), com influência de sedimentos subandinos. Em contraste, a maior parte da FLONA possui solos extremamente ácidos e pobres em nutrientes (distróficos), formados a partir do intemperismo de sedimentos da Formação Solimões. O manejo dessas áreas deve visar ao incremento do aporte de biomassa na superfície dos solos, aumentando a eficiência da ciclagem de nutrientes pela vegetação, já que a reserva química natural é extremamente baixa.<br>The lack of basic studies for the elaboration of management plans has delayed the effective implementation of conservation units in the Amazon region. In this work, the interpretation of orbital and sub-orbital images, together with field surveys were used for the geoenvironmental characterization of the Purus National Forest (PNF). A georreferenced digital database composed of thematic maps was produced and constitutes the base of a GIS for the PNF. Four geoenvironments were identified and mapped: Dissecated Plateaux with forest vegetation on Latosols and Argisols; Forested slopes on Argisols; Alluvial plains with eutric soils; Alluvial plains with dystric soils. The holocenic alluviums influenced by the Purus River are eutric, and receive sub-Andenean sediments. On the other hand, most of the PNF has extremely poor and acid soils related to the Solimões Formation. Since the nutrient levels in the mineral substrate are very low, the management of these areas should seek the increment of biomass in the system, which would enhance nutrient cycling by the vegetation since the nutrient levels in the mineral substrate are very low