Structural and functional characterisation of the fork head transcription factor-encoding gene, Hc-daf-16, from the parasitic nematode Haemonchus contortus (Strongylida)

Despite their phylogenetic diversity, parasitic nematodes share attributes of longevity and developmental arrest (=hypobiosis) with free-living nematodes at key points in their life cycles, particularly in larval stages responsible for establishing infection in the host. Insulin-like signalling plays crucial roles in the regulation of life span and arrest (=dauer formation) in the free-living nematode, Caenorhabditis elegans. Insulin-like signalling in C. elegans negatively regulates the fork head boxO (FoxO) transcription factor encoded by daf-16, which is linked to initiating a dauer-specific pattern of gene expression. Orthologues of daf-16 have been identified in several species of parasitic nematode. Although function has been demonstrated for an orthologue from the parasitic nematode Strongyloides stercoralis (Rhabditida), the functional capabilities of homologues/orthologues in bursate nematodes (Strongylida) are unknown. In the present study, we used a genomic approach to determine the structures of two complete daf-16 orthologues (designated Hc-daf-16.1 and Hc-daf-16.2) and their transcripts in the parasitic nematode Haemonchus contortus, and assessed their function(s) using C. elegans as a genetic surrogate. Unlike the multiple isoforms of Ce-DAF-16 and Ss-DAF-16, which are encoded by a single gene and produced by alternative splicing, mRNAs encoding the proteins Hc-DAF-16.1 and Hc-DAF-16.2 are transcribed from separate and distinct loci. Both orthologues are transcribed in all developmental stages and both sexes of H. contortus, and the inferred proteins (603 and 556 amino acids) each contain a characteristic, highly conserved fork head domain. In spite of distinct differences in genomic organisation compared with orthologues in C. elegans and S. stercoralis, genetic complementation studies demonstrated here that Hc-daf-16.2, but not Hc-daf-16.1, could restore daf-16 function to a C. elegans strain carrying a null mutation at this locus. These findings are consistent with previous results for S. stercoralis and demonstrate functional conservation of the daf-16b orthologue between key parasitic nematodes from two different taxonomic orders and C. elegans. We conclude from these experiments that the fork head transcription factor DAF-16 and, by inference, other insulin-like signalling elements, are conserved in H. contortus, a parasitic nematode of paramount economic importance. We demonstrate that functionality is sufficiently conserved in Hc-DAF-16.2 that it can replace Ce-DAF-16 in promoting dauer arrest in C. elegans

Trans-Planckian signals from the breaking of local Lorentz invariance

This article examines how a breakdown of a locally Lorentz invariant, point-like description of nature at tiny space-time intervals would translate into a distinctive set of signals in the primordial power spectrum generated by inflation. We examine the leading irrelevant operators that are consistent with the spatial translations and rotations of a preferred, isotropically expanding, background. A few of the resulting corrections to the primordial power spectrum do not have the usual oscillatory factor, which is sometimes taken to be characteristic of a "trans-Planckian" signal. Perhaps more interestingly, one of these leading irrelevant operators exactly reproduces a correction to the power spectrum that occurs in effective descriptions of the state of the field responsible for inflation.Comment: 11 pages, no figures, uses ReVTe

The Kuiper Belt Luminosity Function from m(R)=21 to 26

We have performed an ecliptic imaging survey of the Kuiper belt with our deepest and widest field achieving a limiting flux of m(g') = 26.4, with a sky coverage of 3.0 square-degrees. This is the largest coverage of any other Kuiper belt survey to this depth. We detect 72 objects, two of which have been previously observed. We have improved the Bayesian maximum likelihood fitting technique presented in Gladman et al. (1998) to account for calibration and sky density variations and have used this to determine the luminosity function of the Kuiper belt. Combining our detections with previous surveys, we find the luminosity function is well represented by a single power-law with slope alpha = 0.65 +/- 0.05 and an on ecliptic sky density of 1 object per square-degree brighter than m(R)=23.42 +/- 0.13. Assuming constant albedos, this slope suggests a differential size-distribution slope of 4.25 +/- 0.25, which is steeper than the Dohnanyi slope of 3.5 expected if the belt is in a state of collisional equilibrium. We find no evidence for a roll-over or knee in the luminosity function and reject such models brightward of m(R) ~ 24.6.Comment: 50 Pages, 8 Figure

How Efficient Is The Langacker-Pi Mechanism of Monopole Annihilation?

We investigate the dynamics of monopole annihilation by the Langacker-Pi mechanism. We find taht considerations of causality, flux-tube energetics and the friction from Aharonov-Bohm scatteering suggest that the monopole annihilation is most efficient if electromagnetism is spontaneously broken at the lowest temperature ($T_{em} \approx 10^6 GeV$) consistent with not having the monopoles dominate the energy density of the universe.Comment: 10 page

Are Textures Natural?

We make the simple observation that, because of global symmetry violating higher-dimension operators expected to be induced by Planck-scale physics, textures are generically much too short-lived to be of use for large-scale structure formation.Comment: 9p

Irreducible decomposition for tensor prodect representations of Jordanian quantum algebras

Tensor products of irreducible representations of the Jordanian quantum algebras U_h(sl(2)) and U_h(su(1,1)) are considered. For both the highest weight finite dimensional representations of U_h(sl(2)) and lowest weight infinite dimensional ones of U_h(su(1,1)), it is shown that tensor product representations are reducible and that the decomposition rules to irreducible representations are exactly the same as those of corresponding Lie algebras.Comment: LaTeX, 14pages, no figur

HAT-P-6b: A Hot Jupiter transiting a bright F star

In the ongoing HATNet survey we have detected a giant planet, with radius 1.33 +/- 0.06 RJup and mass 1.06 +/- 0.12 MJup, transiting the bright (V = 10.5) star GSC 03239-00992. The planet is in a circular orbit with period 3.852985 +/- 0.000005 days and mid-transit epoch 2,454,035.67575 +/- 0.00028 (HJD). The parent star is a late F star with mass 1.29 +/- 0.06 Msun, radius 1.46 +/- 0.06 Rsun, Teff ~ 6570 +/- 80 K, [Fe=H] = -0.13 +/- 0.08 and age ~ 2.3+/-^{0.5}_{0.7}Gy. With this radius and mass, HAT-P-6b has somewhat larger radius than theoretically expected. We describe the observations and their analysis to determine physical properties of the HAT-P-6 system, and briefly discuss some implications of this finding.Comment: Accepted for publication in ApJL, 5 pages, minor changes compared to V

Minimal Family Unification

Absract It is proposed that there exist, within a new $SU(2)^{'}$, a gauged discrete group $Q_6$ (the order 12 double dihedral group) acting as a family symmetry. This nonabelian finite group can explain hierarchical features of families, using an assignment for quarks and leptons dictated by the requirements of anomaly cancellation and of no additional quarks.Comment: 10 pages, IFP-701-UNC;VAND-TH-94-

Neutrino oscillations and uncertainty relations

We show that coherent flavor neutrino states are produced (and detected) due to the momentum-coordinate Heisenberg uncertainty relation. The Mandelstam-Tamm time-energy uncertainty relation requires non-stationary neutrino states for oscillations to happen and determines the time interval (propagation length) which is necessary for that. We compare different approaches to neutrino oscillations which are based on different physical assumptions but lead to the same expression for the neutrino transition probability in standard neutrino oscillation experiments. We show that a Moessbauer neutrino experiment could allow to distinguish different approaches and we present arguments in favor of the 163Ho-163Dy system for such an experiment.Comment: Some small changes in section 2, results unchanged. Added referenc