1,202 research outputs found
Insulin-Like Peptide Signaling in Mosquitoes: The Road Behind and the Road Ahead
Insulin signaling is a conserved pathway in all metazoans. This pathway contributed toward primordial metazoans responding to a greater diversity of environmental signals by modulating nutritional storage, reproduction, and longevity. Most of our knowledge of insulin signaling in insects comes from the vinegar fly, Drosophila melanogaster, where it has been extensively studied and shown to control several physiological processes. Mosquitoes are the most important vectors of human disease in the world and their control constitutes a significant area of research. Recent studies have shown the importance of insulin signaling in multiple physiological processes such as reproduction, innate immunity, lifespan, and vectorial capacity in mosquitoes. Although insulin-like peptides have been identified and functionally characterized from many mosquito species, a comprehensive review of this pathway in mosquitoes is needed. To fill this gap, our review provides up-to-date knowledge of this subfield
Magnetic properties of PdAs2O6: a dilute spin system with an unusually high N\'eel temperature
The crystal structure and magnetic ordering pattern of PdAs2O6 were
investigated by neutron powder diffraction. While the magnetic structure of
PdAs2O6 is identical to the one of its isostructural 3d-homologue NiAs2O6, its
N\'{e}el temperature (140 K) is much higher than the one of NiAs2O6 (30 K).
This is surprising in view of the long distance and indirect exchange path
between the magnetic Pd ions. Density functional calculations yield
insight into the electronic structure and the geometry of the exchange-bond
network of both PdAs2O6 and NiAs2O6, and provide a semi-quantitative
explanation of the large amplitude difference between their primary exchange
interaction parameters
Dynamics of Insulin Signaling in the Black-Legged Tick, Ixodes scapularis
Insulin-like peptides (ILPs) have been identified in several invertebrates, particularly insects, and work on these ILPs has revealed many roles including regulation of energy homeostasis, growth, development, and lifespan to name a few. However, information on arthropod ILPs outside of insects is sparse. Studies of Ixodid tick ILPs are particularly scarce, despite their importance as vectors of infectious agents, most notably Lyme disease. The recent publication of the genome of the black-legged tick, Ixodes scapularis, has advanced opportunities to study this organism from a molecular standpoint, a resource sorely needed for an organism with challenging life history requirements for study in the laboratory, such as a long life cycle and obligate, prolonged, blood-feeding at each life stage. Through bioinformatics searches of the tick genome and other available I. scapularis databases, we identified four putative ILP sequences. Full-length sequences of these ILP transcripts were confirmed, and quantitative RT-PCR was used to examine expression levels of these ILPs in different life stages, feeding states, and adult tissues. This work serves as an initial characterization of ILP expression in ticks and provides the foundation for further exploration of the roles of ILPs in these important arthropod vectors
Distribution of Neuropeptide F-Like Immunoreactivity in the Eastern Subterranean Termite, Reticulitermes flavipes
The nervous system and gut of worker, soldier and alate castes of the eastern subterranean termite, Reticulitermes flavipes Kollar (Isoptera: Rhinotermitidae) were examined for immunoreactivity to an antiserum to Helicoverpa zea (Boddie) (Leipidoptera: Noctuidae) MP-I (QAARPRF-NH2), a truncated form of neuropeptide F. More than 145 immunostained axons and cell bodies were seen in the brain and all ganglia of the ventral nerve cord. Immunoreactive axons exiting the brain projected anteriorly to the frontal ganglion and posteriorly to the corpora cardiaca and corpora allata. In the stomatogastric nervous system, immunoreactive axons were observed over the surface of the foregut, salivary glands, midgut and rectum. These axons originated in the brain and from 15–25 neurosecretory cells on the foregut. Staining patterns were consistent between castes, with the exception of immunostaining observed in the optic lobes of alates. At least 600 immunoreactive endocrine cells were evenly distributed in the midguts of all castes with higher numbers present in the worker caste. Immunostaining of cells in the nervous system and midgut was blocked by preabsorption of the antiserum with Hez MP-I but not by a peptide having only the RF-NH2 in common. This distribution suggests NPF-like peptides coordinate feeding and digestion in all castes of this termite species
Genomic Insights Into the Ixodes Scapularis Tick Vector of Lyme Disease
Ticks transmit more pathogens to humans and animals than any other arthropod. We describe the 2.1 Gbp nuclear genome of the tick, Ixodes scapularis (Say), which vectors pathogens that cause Lyme disease, human granulocytic anaplasmosis, babesiosis and other diseases. The large genome reflects accumulation of repetitive DNA, new lineages of retro-transposons, and gene architecture patterns resembling ancient metazoans rather than pancrustaceans. Annotation of scaffolds representing ~57% of the genome, reveals 20,486 protein-coding genes and expansions of gene families associated with tick-host interactions. We report insights from genome analyses into parasitic processes unique to ticks, including host \u27questing\u27, prolonged feeding, cuticle synthesis, blood meal concentration, novel methods of haemoglobin digestion, haem detoxification, vitellogenesis and prolonged off-host survival. We identify proteins associated with the agent of human granulocytic anaplasmosis, an emerging disease, and the encephalitis-causing Langat virus, and a population structure correlated to life-history traits and transmission of the Lyme disease agent
Recovery of low volumes of wear debris from rat stifle joint tissues using a novel particle isolation method
Less than optimal particle isolation techniques have impeded analysis of orthopaedic wear debris in vivo. The purpose of this research was to develop and test an improved method for particle isolation from tissue. A volume of 0.018 mm³ of clinically relevant CoCrMo, Ti-6Al-4V or Si₃N₄ particles was injected into rat stifle joints for seven days of in vivo exposure. Following sacrifice, particles were located within tissues using histology. The particles were recovered by enzymatic digestion of periarticular tissue with papain and proteinase K, followed by ultracentrifugation using a sodium polytungstate density gradient. Particles were recovered from all samples, observed using SEM and the particle composition was verified using EDX, which demonstrated that all isolated particles were free from contamination. Particle size, aspect ratio and circularity were measured using image analysis software. There were no significant changes to the measured parameters of CoCrMo or Si₃N₄ particles before and after the recovery process (KS tests, p > 0.05). Titanium particles were too few before and after isolation to analyse statistically, though size and morphologies were similar. Overall the method demonstrated a significant improvement to current particle isolation methods from tissue in terms of sensitivity and efficacy at removal of protein, and has the potential to be used for the isolation of ultra-low wearing total joint replacement materials from periprosthetic tissues
Spin waves and spin-state transitions in a ruthenate high-temperature antiferromagnet
Ruthenium compounds play prominent roles in materials research ranging from
oxide electronics to catalysis, and serve as a platform for fundamental
concepts such as spin-triplet superconductivity, Kitaev spin-liquids, and
solid-state analogues of the Higgs mode in particle physics. However, basic
questions about the electronic structure of ruthenates remain unanswered,
because several key parameters (including the Hund's-rule, spin-orbit, and
exchange interactions) are comparable in magnitude, and their interplay is
poorly understood - partly due to difficulties in synthesizing sizable single
crystals for spectroscopic experiments. Here we introduce a resonant inelastic
x-ray scattering (RIXS) technique capable of probing collective modes in
microcrystals of -electron materials. We present a comprehensive set of
data on spin waves and spin-state transitions in the honeycomb antiferromagnet
SrRuO, which possesses an unusually high N\'eel temperature. The
new RIXS method provides fresh insight into the unconventional magnetism of
SrRuO, and enables momentum-resolved spectroscopy of a large class
of transition-metal compounds.Comment: The original submitted version of the published manuscript.
https://www.nature.com/articles/s41563-019-0327-
- …