Farmers’ management of functional biodiversity goes beyond pest management in organic European apple orchards

Supporting functional biodiversity (FB), which provides natural pest regulation, is an environmentally sound and promising approach to reduce pesticide use in perennial cultures such as apple, especially in organic farming. However, little is known about farmers’ practices and motivations to implement techniques that favor FB, especially whether or not they really expect anything from FB in terms of pest regulation. In fact, FB-supporting techniques (FB-techniques) are massively questioned by practitioners due to inadequate information about their effectiveness. An interview survey was performed in eight European countries(i) to describe farmers’ practices and identify promising FB-techniques: (ii) to better understand their perceptions of and values associated with FB; and (iii) to identify potential drivers of (non-)adoption. Fifty-five advisors and 125 orchard managers with various degrees of experience and convictions about FB were interviewed and a total of 24 different FB-techniques which can be assigned to three different categories (ecological infrastructures, farming practices and redesign techniques) were described. Some were well-established measures (e.g., hedges and bird houses), while others were more marginal and more recent (e.g., animal introduction and compost). On average, farmers combined more than four techniques that had been implemented over a period of 13 years, especially during their establishment or conversion period. In general, it was difficult for farmers to evaluate the effectiveness of individual FB-techniques on pest regulation. They considered FB-techniques as a whole, targeting multiple species, and valued multiple ecosystem services in addition to pest regulation. The techniques implemented and their associated values differed among farmers who adopted various approaches towards FB. Three different approaches were defined: passive, active and integrated. Their appraisal of FB is even more complex because it may change with time and experience. These findings provide empirical evidence that the practical implementation of promising techniques remains a challenge, considering the diversity of situations and evaluation criteria. Increased cooperation between researchers, farmers and advisors should more effectively target research, advisory support and communication to meet farmers’ needs and perceptions

The Milky Way: An Exceptionally Quiet Galaxy; Implications for the formation of spiral galaxies

[Abridged]We compare both the Milky Way and M31 galaxies to local external disk galaxies within the same mass range, using their relative locations in the planes formed by V_flat versus M_K, j_disk, and the average Fe abundance of stars in the galaxy outskirts. We find, for all relationships, that the MW is systematically offset by ~ 1 sigma, showing a significant deficiency in stellar mass, in angular momentum, in disk radius and [Fe/H] in the stars in its outskirts at a given V_flat. On the basis of their location in the M_K, V_flat, and R_d volume, the fraction of spirals like the MW is 7+/-1%, while M31 appears to be a "typical'' spiral. Our Galaxy appears to have escaped any significant merger over the last ~10 Gyrs which may explain why it is deficient by a factor 2 to 3 in stellar mass, angular momentum and outskirts metallicity and then, unrepresentative of the typical spiral. As with M31, most local spirals show evidence for a history shaped mainly by relatively recent merging. We conclude that the standard scenario of secular evolution is generally unable to reproduce the properties of most (if not all) spiral galaxies. However, the so-called "spiral rebuilding'' scenario proposed by Hammer et al. 2005 is consistent with the properties of both distant galaxies and of their descendants - the local spirals.Comment: 14 pages, 6 figures, to appear in Ap

Gravitational wave searches for ultralight bosons with LIGO and LISA

Ultralight bosons can induce superradiant instabilities in spinning black holes, tapping their rotational energy to trigger the growth of a bosonic condensate. Possible observational imprints of these boson clouds include (i) direct detection of the nearly monochromatic (resolvable or stochastic) gravitational waves emitted by the condensate, and (ii) statistically significant evidence for the formation of \u201choles\u201d at large spins in the spin versus mass plane (sometimes also referred to as \u201cRegge plane\u201d) of astrophysical black holes. In this work, we focus on the prospects of LISA and LIGO detecting or constraining scalars with mass in the range ms 08[10 1219,10 1215]\u2009\u2009eV and ms 08[10 1214,10 1211]\u2009\u2009eV, respectively. Using astrophysical models of black-hole populations calibrated to observations and black-hole perturbation theory calculations of the gravitational emission, we find that, in optimistic scenarios, LIGO could observe a stochastic background of gravitational radiation in the range ms 08[2 710 1213,10 1212]\u2009\u2009eV, and up to 104 resolvable events in a 4-year search if ms 3c3 710 1213\u2009\u2009eV. LISA could observe a stochastic background for boson masses in the range ms 08[5 710 1219,5 710 1216], and up to 3c103 resolvable events in a 4-year search if ms 3c10 1217\u2009\u2009eV. LISA could further measure spins for black-hole binaries with component masses in the range [103,107]M 99, which is not probed by traditional spin-measurement techniques. A statistical analysis of the spin distribution of these binaries could either rule out scalar fields in the mass range 3c[4 710 1218,10 1214]\u2009\u2009eV, or measure ms with ten percent accuracy if light scalars in the mass range 3c[10 1217,10 1213]\u2009\u2009eV exist

An Infant Formula with Partially Hydrolyzed Whey Protein Supports Adequate Growth and Is Safe and Well-Tolerated in Healthy, Term Infants: A Randomized, Double-Blind, Equivalence Trial

The current study evaluates the safety and tolerance of a partially hydrolyzed whey protein-based infant formula (PHF) versus an in intact cow's milk protein formula (IPF). Breastfed infants were included as a reference group. In a multi-country, multicenter, randomized, double-blinded, controlled clinical trial, infants whose mothers intended to fully formula feed were randomized to PHF (n= 134) or IPF (n= 134) from <= 14 days to 17 weeks of age. The equivalence analysis of weight gain per day within margins of +/-3 g/d (primary outcome), the recorded adverse events, growth and gastro-intestinal tolerance parameters were considered for the safety evaluation. Equivalence of weight gain per day from enrolment until 17 weeks of age was demonstrated in the PHF group compared to the IPF group (difference in means -1.2 g/d; 90% CI (-2.42; 0.02)), with estimated means (SE) of 30.2 (0.5) g/d and 31.4 (0.5) g/d, respectively. No significant differences in growth outcomes, the number, severity or type of (serious) adverse events and tolerance outcomes, were observed between the two formula groups. A partially hydrolyzed whey protein-based infant formula supports adequate infant growth, with a daily weight gain equivalent to a standard intact protein-based formula; it is also safe for use and well-tolerated in healthy term infants

Análisis de la aplicabilidad del modelo de Bohr acerca de la predicción de las líneas de emisión del átomo de helio en el marco del dictado de Física Moderna en la Facultad de Ingeniería de la UNLP

El dictado de la materia Física IIIA de las carreras de Ingeniería Electrónica (IE1), Electricista (IE2) y Química (IQ) dictadas en la Facultad de Ingeniería (FI) de la Universidad Nacional de La Plata (UNLP) abarca dos módulos bimestrales. En el segundo de ellos se abordan temas de Física Moderna y entre las actividades de laboratorio establecidas, los alumnos deben realizar un experimento destinado a determinar las líneas características de emisión de una lámpara de gas de He a baja presión utilizando un espectrómetro portátil. En el presente trabajo se describe el instrumental utilizado, la metodología experimental adoptada, se presentan las mediciones realizadas de las líneas características emitidas por el átomo He (HeI) y se las compara con las líneas publicadas en la literatura. A partir del uso del modelo de Bohr para el caso del átomo de He+1 simplemente ionizado (HeII), se estiman las longitudes de onda emitidas por el ión HeII y se las compara con las líneas características del HeII publicadas. También en el marco del modelo de Bohr, se calculan las longitudes de onda emitidas por el átomo HeI despreciando la repulsión entre los electrones y se las compara con las líneas observadas en el laboratorio. Finalmente, se analiza la aplicabilidad del modelo de Bohr en la descripción de los sistemas HeI y HeII.The Physic IIIA course of the Electronic, Electric and Chemical Engineering degrees of La Plata University includes two modules of two months each. The second teaching module includes Modern Physic topics. In special, in the laboratory activities, students carry out an experiment to determine the characteristic emission lines of an He lamp using a didactic spectrometer. In the present work, we describe the approach used in the laboratory and the experimental methodology. We present the emission lines measured and we compare these measurements with the He emission lines reported in the literature. From the Bohr model, we estimate the emitted wave length for the case of the He+1 ion (The He atom without an electron) and we compare these wave lengths with the lines published. In this sense, we also calculate the emitted lines of the He atom in the frame of the Bohr model and we compare these calculations with the lines measured in the laboratory. Finally, we analyze the potential application of the Bohr model in the description of the He atom and of the He+1 ion.Trabajo presentado en la 96A Reunión Nacional de Física y II Reunión Conjunta Sociedad Uruguaya de Física - AsociacióN Física Argentin

Diagnostic Accuracy of Adenosine Deaminase and Lymphocyte Proportion in Pleural Fluid for Tuberculous Pleurisy in Different Prevalence Scenarios

BACKGROUND: Tuberculous pleural effusion (TPE) is a paucibacillary manifestation of tuberculosis, so isolation of Mycobacterium tuberculosis is difficult, biomarkers being an alternative for diagnosis. Adenosine deaminase (ADA) is the most cost-effective pleural fluid marker and is routinely used in high prevalence settings, whereas its value is questioned in areas with low prevalence. The lymphocyte proportion (LP) is known to increase the specificity of ADA for this diagnosis. We analyse the diagnostic usefulness of ADA alone and the combination of ADA ≥ 40 U/l (ADA(40)) and LP ≥ 50% (LP(50)) in three different prevalence scenarios over 11 years in our area. MATERIALS AND METHODS: Biochemistry, cytology and microbiology studies from 472 consecutive pleural fluid samples were retrospectively analyzed. ADA and differential cell count were determined in all samples. We established three different prevalence periods, based on percentage of pleural effusion cases diagnosed as tuberculosis: 1998-2000 (31.3%), 2001-2004 (11.8%), and 2005-2008 (7.4%). ROC curves, dispersion diagrams and pre/post-test probability graphs were produced. TPE accounted for 73 episodes (mean prevalence: 15.5%). The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) for ADA(40) were 89%, 92.7%, 69.2% and 97.9%, respectively. For ADA(40)+LP(50) the specificity and PPV increased (98.3% and 90%) with hardly any decrease in the sensitivity or NPV (86.3% and 97.5%). No relevant differences were observed between the three study periods. CONCLUSIONS/SIGNIFICANCE: ADA remains useful for the diagnosis of TPE even in low-to-intermediate prevalence scenarios when combined with the lymphocyte proportion

Free-standing polyelectrolyte membranes made of chitosan and alginate

Free-standing films have increasing applications in the biomedical field as drug delivery systems for wound healing and tissue engineering. Here, we prepared free-standing membranes by the layer-by-layer assembly of chitosan and alginate, two widely used biomaterials. Our aim was to produce a thick membrane and to study the permeation of model drugs and the adhesion of muscle cells. We first defined the optimal growth conditions in terms of pH and alginate concentration. The membranes could be easily detached from polystyrene or polypropylene substrate without any postprocessing step. The dry thickness was varied over a large range from 4 to 35 μm. A 2-fold swelling was observed by confocal microscopy when they were immersed in PBS. In addition, we quantified the permeation of model drugs (fluorescent dextrans) through the free-standing membrane, which depended on the dextran molecular weight. Finally, we showed that myoblast cells exhibited a preferential adhesion on the alginate-ending membrane as compared to the chitosan-ending membrane or to the substrate side.This work was financially supported by Foundation for Science and Technology (FCT) through the Scholarship SFRH/BD/64601/2009 granted to S.G.C. C.M. is indebted to Grenoble INP for financial support via a postdoctoral fellowship. This work was supported by the European Commission (FP7 Program) via a European Research Council starting grant (BIOMIM, GA 259370 to C.P.). C.P. is also grateful to Institut Universitaire de France and to Grenoble Institute of Technology for financial support. We thank Isabelle Paintrand for her technical help with the confocal apparatus and Patrick Chaudouet for his help with SEM imaging

SLITRK2, an X-linked modifier of the age at onset in C9orf72 frontotemporal lobar degeneration

The G4C2-repeat expansion in C9orf72 is the most common cause of frontotemporal dementia and of amyotrophic lateral sclerosis. The variability of age at onset and phenotypic presentations is a hallmark of C9orf72 disease. In this study, we aimed to identify modifying factors of disease onset in C9orf72 carriers using a family-based approach, in pairs of C9orf72 carrier relatives with concordant or discordant age at onset. Linkage and association analyses provided converging evidence for a locus on chromosome Xq27.3. The minor allele A of rs1009776 was associated with an earlier onset (P = 1 × 10-5). The association with onset of dementia was replicated in an independent cohort of unrelated C9orf72 patients (P = 0.009). The protective major allele delayed the onset of dementia from 5 to 13 years on average depending on the cohort considered. The same trend was observed in an independent cohort of C9orf72 patients with extreme deviation of the age at onset (P = 0.055). No association of rs1009776 was detected in GRN patients, suggesting that the effect of rs1009776 was restricted to the onset of dementia due to C9orf72. The minor allele A is associated with a higher SLITRK2 expression based on both expression quantitative trait loci (eQTL) databases and in-house expression studies performed on C9orf72 brain tissues. SLITRK2 encodes for a post-synaptic adhesion protein. We further show that synaptic vesicle glycoprotein 2 and synaptophysin, two synaptic vesicle proteins, were decreased in frontal cortex of C9orf72 patients carrying the minor allele. Upregulation of SLITRK2 might be associated with synaptic dysfunctions and drives adverse effects in C9orf72 patients that could be modulated in those carrying the protective allele. How the modulation of SLITRK2 expression affects synaptic functions and influences the disease onset of dementia in C9orf72 carriers will require further investigations. In summary, this study describes an original approach to detect modifier genes in rare diseases and reinforces rising links between C9orf72 and synaptic dysfunctions that might directly influence the occurrence of first symptoms