Deduced Primary Structure of the β Subunit of Brain Type II Ca2+/calmodulin-dependent Protein Kinase Determined by Molecular Cloning

cDNA clones coding for the β subunit of rat brain type II Ca2+/calmodulin-dependent protein kinase were isolated and sequenced. The clones, including one containing the entire coding region, hybridize at high stringency to a single band of poly(A)+ RNA of length 4.8 kilobases. The subunit coded for by the clones was identified by in vitro transcription of the cDNA followed by translation of the resulting RNA. The DNA sequence of the clones contains a single long open reading frame (1626 nucleotides) coding for a protein of 542 amino acids with a molecular weight of 60,333, the amino-terminal half of which is homologous to several other protein kinases. Potential ATP- and calmodulin-binding domains were identified. Two independent clones contain an identical 45-nucleotide deletion, relative to the clones described above, resulting in a shorter open reading frame coding for a protein of molecular weight 58,000. This suggests that the minor, 58-kDa β' subunit of the type II Ca2+/calmodulin-dependent kinase may be synthesized on a separate message

Purification and characterization of a calmodulin-dependent protein kinase that is highly concentrated in brain

A calcium and calmodulin-dependent protein kinase has been purified from rat brain. It was monitored during the purification by its ability to phosphorylate the synaptic vesicle-associated protein, synapsin I. A 300-fold purification was sufficient to produce kinase that is 90-95% pure as determined by scans of stained sodium dodecyl sulfate-polyacrylamide gels and has a specific activity of 2.9 mumol of 32P transferred per min/mg of protein. Thus, the kinase is a relatively abundant brain enzyme, perhaps comprising as much as 0.3% of the total brain protein. The Stokes radius (95 A) and sedimentation coefficient (16.4 S) of the kinase indicate a holoenzyme molecular weight of approximately 650,000. The holoenzyme is composed of three subunits as judged by their co-migration with kinase activity during the purification steps and co-precipitation with kinase activity by a specific anti-kinase monoclonal antibody. The three subunits have molecular weights of 50,000, 58,000, and 60,000, and have been termed alpha, beta', and beta, respectively. The alpha- and beta-subunits are distinct peptides, however, beta' may have been generated from beta by proteolysis. All three of these subunits bind calmodulin in the presence of calcium and are autophosphorylated under conditions in which the kinase is active. The subunits are present in a ratio of about 3 alpha-subunits to 1 beta/beta'-subunit. We therefore postulate that the 650,000-Da holoenzyme consists of approximately 9 alpha-subunits and 3 beta/beta'-subunits. The abundance of this calmodulin-dependent protein kinase indicates that its activation is likely to be an important biochemical response to increases in calcium ion concentration in neuronal tissue

Lattice-corrected strain-induced vector potentials in graphene

The electronic implications of strain in graphene can be captured at low energies by means of pseudovector potentials which can give rise to pseudomagnetic fields. These strain-induced vector potentials arise from the local perturbation to the electronic hopping amplitudes in a tight-binding framework. Here we complete the standard description of the strain-induced vector potential, which accounts only for the hopping perturbation, with the explicit inclusion of the lattice deformations or, equivalently, the deformation of the Brillouin zone. These corrections are linear in strain and are different at each of the strained, inequivalent Dirac points, and hence are equally necessary to identify the precise magnitude of the vector potential. This effect can be relevant in scenarios of inhomogeneous strain profiles, where electronic motion depends on the amount of overlap among the local Fermi surfaces. In particular, it affects the pseudomagnetic field distribution induced by inhomogeneous strain configurations, and can lead to new opportunities in tailoring the optimal strain fields for certain desired functionalities.Comment: Errata for version

Comparing primary prevention with secondary prevention to explain decreasing Coronary Heart Disease death rates in Ireland, 1985-2000.

BACKGROUND: To investigate whether primary prevention might be more favourable than secondary prevention (risk factor reduction in patients with coronary heart disease(CHD)). METHODS: The cell-based IMPACT CHD mortality model was used to integrate data for Ireland describing CHD patient numbers, uptake of specific treatments, trends in major cardiovascular risk factors, and the mortality benefits of these specific risk factor changes in CHD patients and in healthy people without recognised CHD. RESULTS: Between 1985 and 2000, approximately 2,530 fewer deaths were attributable to reductions in the three major risk factors in Ireland. Overall smoking prevalence declined by 14% between 1985 and 2000, resulting in about 685 fewer deaths (minimum estimate 330, maximum estimate 1,285) attributable to smoking cessation: about 275 in healthy people and 410 in known CHD patients. Population total cholesterol concentrations fell by 4.6%, resulting in approximately 1,300 (minimum estimate 1,115, maximum estimate 1,660) fewer deaths attributable to dietary changes(1,185 in healthy people and 115 in CHD patients) plus 305 fewer deaths attributable to statin treatment (45 in people without CHD and 260 in CHD patients). Mean population diastolic blood pressure fell by 7.2%, resulting in approximately 170 (minimum estimate 105, maximum estimate 300) fewer deaths attributable to secular falls in blood pressure (140 in healthy people and 30 in CHD patients), plus approximately 70 fewer deaths attributable to antihypertensive treatments in people without CHD. Of all the deaths attributable to risk factor falls, some 1,715 (68%) occurred in people without recognized CHD and 815(32%) in CHD patients. CONCLUSION: Compared with secondary prevention, primary prevention achieved a two-fold larger reduction in CHD deaths. Future national CHD policies should therefore prioritize nationwide interventions to promote healthy diets and reduce smoking

Remote State Preparation

Quantum teleportation uses prior entanglement and forward classical communication to transmit one instance of an unknown quantum state. Remote state preparation (RSP) has the same goal, but the sender knows classically what state is to be transmitted. We show that the asymptotic classical communication cost of RSP is one bit per qubit - half that of teleportation - and becomes even less when transmitting part of a known entangled state. We explore the tradeoff between entanglement and classical communication required for RSP, and discuss RSP capacities of general quantum channels.Comment: 4 pages including 1 epsf figure; v3 has an additional author and discusses relation to work of Devetak and Berger (quant-ph/0102123); v4 improves low-entanglement protocols without back communication to perform as well as low-entanglement protocols with back communication; v5 (journal version) has a few small change

The Parity Bit in Quantum Cryptography

An $n$-bit string is encoded as a sequence of non-orthogonal quantum states. The parity bit of that $n$-bit string is described by one of two density matrices, $\rho_0^{(n)}$ and $\rho_1^{(n)}$, both in a Hilbert space of dimension $2^n$. In order to derive the parity bit the receiver must distinguish between the two density matrices, e.g., in terms of optimal mutual information. In this paper we find the measurement which provides the optimal mutual information about the parity bit and calculate that information. We prove that this information decreases exponentially with the length of the string in the case where the single bit states are almost fully overlapping. We believe this result will be useful in proving the ultimate security of quantum crytography in the presence of noise.Comment: 19 pages, RevTe

Security against eavesdropping in quantum cryptography

In this article we deal with the security of the BB84 quantum cryptography protocol over noisy channels using generalized privacy amplification. For this we estimate the fraction of bits needed to be discarded during the privacy amplification step. This estimate is given for two scenarios, both of which assume the eavesdropper to access each of the signals independently and take error correction into account. One scenario does not allow a delay of the eavesdropper's measurement of a measurement probe until he receives additional classical information. In this scenario we achieve a sharp bound. The other scenario allows a measurement delay, so that the general attack of an eavesdropper on individual signals is covered. This bound is not sharp but allows a practical implementation of the protocol.Comment: 11 pages including 3 figures, contains new results not contained in my Phys. Rev. A pape

Two qubit copying machine for economical quantum eavesdropping

We study the mapping which occurs when a single qubit in an arbitrary state interacts with another qubit in a given, fixed state resulting in some unitary transformation on the two qubit system which, in effect, makes two copies of the first qubit. The general problem of the quality of the resulting copies is discussed using a special representation, a generalization of the usual Schmidt decomposition, of an arbitrary two-dimensional subspace of a tensor product of two 2-dimensional Hilbert spaces. We exhibit quantum circuits which can reproduce the results of any two qubit copying machine of this type. A simple stochastic generalization (using a ``classical'' random signal) of the copying machine is also considered. These copying machines provide simple embodiments of previously proposed optimal eavesdropping schemes for the BB84 and B92 quantum cryptography protocols.Comment: Minor changes. 26 pages RevTex including 7 PS figure