Hyperpolarized 13C-pyruvate MRI detects real-time metabolic flux in prostate cancer metastases to bone and liver: a clinical feasibility study.

BackgroundHyperpolarized (HP) 13C-pyruvate MRI is a stable-isotope molecular imaging modality that provides real-time assessment of the rate of metabolism through glycolytic pathways in human prostate cancer. Heretofore this imaging modality has been successfully utilized in prostate cancer only in localized disease. This pilot clinical study investigated the feasibility and imaging performance of HP 13C-pyruvate MR metabolic imaging in prostate cancer patients with metastases to the bone and/or viscera.MethodsSix patients who had metastatic castration-resistant prostate cancer were recruited. Carbon-13 MR examination were conducted on a clinical 3T MRI following injection of 250 mM hyperpolarized 13C-pyruvate, where pyruvate-to-lactate conversion rate (kPL) was calculated. Paired metastatic tumor biopsy was performed with histopathological and RNA-seq analyses.ResultsWe observed a high rate of glycolytic metabolism in prostate cancer metastases, with a mean kPL value of 0.020 ± 0.006 (s-1) and 0.026 ± 0.000 (s-1) in bone (N = 4) and liver (N = 2) metastases, respectively. Overall, high kPL showed concordance with biopsy-confirmed high-grade prostate cancer including neuroendocrine differentiation in one case. Interval decrease of kPL from 0.026 at baseline to 0.015 (s-1) was observed in a liver metastasis 2 months after the initiation of taxane plus platinum chemotherapy. RNA-seq found higher levels of the lactate dehydrogenase isoform A (Ldha,15.7 ± 0.7) expression relative to the dominant isoform of pyruvate dehydrogenase (Pdha1, 12.8 ± 0.9).ConclusionsHP 13C-pyruvate MRI can detect real-time glycolytic metabolism within prostate cancer metastases, and can measure changes in quantitative kPL values following treatment response at early time points. This first feasibility study supports future clinical studies of HP 13C-pyruvate MRI in the setting of advanced prostate cancer

Granularity-induced gapless superconductivity in NbN films: evidence of thermal phase fluctuations

Using a single coil mutual inductance technique, we measure the low temperature dependence of the magnetic penetration depth in superconducting NbN films prepared with similar critical temperatures around 16 K but with different microstructures. Only (100) epitaxial and weakly granular (100) textured films display the characteristic exponential dependence of conventional BCS s-wave superconductors. More granular (111) textured films exhibit a linear dependence, indicating a gapless state in spite of the s-wave gap. This result is quantitatively explained by a model of thermal phase fluctuations favored by the granular structure.Comment: 10 pages, 4 figures, to appear in Phys. Rev.

And in the Darkness Bind Them: Equatorial Rings, B[e] Supergiants, and the Waists of Bipolar Nebulae

We report the discovery of two new circumstellar ring nebulae in the western Carina Nebula. The brighter object, SBW1, resembles a lidless staring eye and encircles a B1.5 Iab supergiant. Its size is identical to the inner ring around SN1987A, but SBW1's low N abundance indicates that the star didn't pass through a RSG phase. The fainter object, SBW2, is a more distorted ring, is N-rich, and has a central star that seems to be invisible. We discuss these two new nebulae in context with rings around SN1987A, Sher25, HD168625, RY Scuti, WeBo1, SuWt2, and others. The ring bearers fall into two groups: Five rings surround hot supergiants, and all except for the one known binary are carbon copies of the ring around SN1987A. We propose a link between these rings and B[e] supergiants, where the rings derive from the same material in an earlier B[e] phase. The remaining four rings surround evolved intermediate-mass stars; all members of this ring fellowship are close binaries, hinting that binary interactions govern the forging of such rings. We estimate that there may be several thousand more dark rings in the Galaxy, but we are scarcely aware of their existence due to selection effects. The lower-mass objects might be the equatorial density enhancements often invoked to bind the waists of bipolar PNe.Comment: AJ accepted, 27 page

Shell formation and star formation in superbubble DEM 192

Was star formation in the OB associations, LH 51 and LH 54, triggered by the growth of the superbubble DEM 192? To examine this possibility, we investigate the stellar contents and star formation history, and model the evolution of the shell. H-R diagrams constructed from UBV photometry and spectral classifications indicate highly coeval star formation, with the entire massive star population having an age of ~< 2-3 Myr. However, LH 54 is constrained to an age of ~3 Myr by the presence of a WR star, and the IMF for LH 51 suggests a lower-mass limit implying an age of 1-2 Myr. There is no evidence of an earlier stellar population to create the superbubble, but the modeled shell kinematics are consistent with an origin due to the strongest stellar winds of LH 54. It might therefore be possible that LH 54 created the superbubble, which in turn may have triggered the creation of LH 51. Within the errors, the spatial distribution of stellar masses and IMF appear uniform within the associations. We reinvestigate the estimates for stellar wind power L_w(t), during the H-burning phase, and note that revised mass-loss rates yield a significantly different form for L_w(t), and may affect stellar evolution timescales. We also model superbubble expansion into an ambient medium with a sudden, discontinuous drop in density, and find that this can easily reproduce the anomalously high shell expansion velocities seen in many superbubbles.Comment: 18 pages, 12 figures, AASTeX; Figure 1 bitmapped. Accepted to AJ. Table of Contents and preprint, including hi-resolution version of Figure 1, available at: http://www.ast.cam.ac.uk/~oey/oeypubs.htm

Detection of Coxiella burnetii in complex matrices by using multiplex quantitative PCR during a major Q fever outbreak in the Netherlands

Q fever, caused by Coxiella burnetii, is a zoonosis with a worldwide distribution. A large rural area in the southeast of the Netherlands was heavily affected by Q fever between 2007 and 2009. This initiated the development of a robust and internally controlled multiplex quantitative PCR (qPCR) assay for the detection of C. burnetii DNA in veterinary and environmental matrices on suspected Q fever-affected farms. The qPCR detects three C. burnetii targets (icd, com1, and IS1111) and one Bacillus thuringiensis internal control target (cry1b). Bacillus thuringiensis spores were added to samples to control both DNA extraction and PCR amplification. The performance of the qPCR assay was investigated and showed a high efficiency; a limit of detection of 13.0, 10.6, and 10.4 copies per reaction for the targets icd, com1, and IS1111, respectively; and no crossreactivity with the nontarget organisms tested. Screening for C. burnetii DNA on 29 suspected Q fever-affected farms during the Q fever epidemic in 2008 showed that swabs from dust-accumulating surfaces contained higher levels of C. burnetii DNA than vaginal swabs from goats or sheep. PCR inhibition by coextracted substances was observed in some environmental samples, and 10- or 100-fold dilutions of samples were sufficient to obtain interpretable signals for both the C. burnetii targets and the internal control. The inclusion of an internal control target and three C. burnetii targets in one multiplex qPCR assay showed that complex veterinary and environmental matrices can be screened reliably for the presence of C. burnetii DNA during an outbreak. © 2011, American Society for Microbiology

Comments on the d-wave pairing mechanism for cuprate high TcT_c superconductors: Higher is different?

The question of pairing glue for the cuprate superconductors (SC)is revisited and its determination through the angle resolved photo-emission spectroscopy (ARPES) is discussed in detail. There are two schools of thoughts about the pairing glue question: One argues that superconductivity in the cuprates emerges out of doping the spin singlet resonating valence bond (RVB) state. Since singlet pairs are already formed in the RVB state there is no need for additional boson glue to pair the electrons. The other instead suggests that the d-wave pairs are mediated by the collective bosons like the conventional low $T_c$ SC with the alteration that the phonons are replaced by another kind of bosons ranging from the antiferromagnetic (AF) to loop current fluctuations. An approach to resolve this dispute is to determine the frequency and momentum dependences of the diagonal and off-diagonal self-energies directly from experiments like the McMillan-Rowell procedure for the conventional SC. In that a simple d-wave BCS theory describes superconducting properties of the cuprates well, the Eliashberg analysis of well designed high resolution experimental data will yield the crucial frequency and momentum dependences of the self-energies. This line of approach using ARPES are discussed in more detail in this review, and some remaining problems are commented.Comment: Invited review article published in the Journal of Korean Physical Society; several typos corrected and a few comments and references adde

Discovery of a Dusty Ring in the Coalsack: A Dense Core Caught in the Act of Formation?

We present a new infrared extinction study of Globule 2, the most opaque molecular cloud core in the Coalsack complex. Using deep near-infrared imaging observations obtained with the ESO NTT we are able to examine the structure of the globule in significantly greater detail than previously possible. We find the most prominent structural feature of this globule to be a strong central ring of dust column density which was not evident in lower resolution studies of this cloud. This ring represents a region of high density and pressure that is likely a transient structure. For a spherical cloud geometry the ring would correspond to a dense inner shell of high pressure that could not be in dynamical equilibrium with its surroundings since there appear to be no sources of pressure in the central regions of the cloud that could support the shell against gravity and prevent its inward implosion. The timescale for the inward collapse of the ring would be less than 2 x 10^5 years, suggesting that this globule is in an extremely early stage of evolution, and perhaps caught in the process of forming a centrally condensed dense core or Bok globule. Outside its central regions the globule displays a well-behaved density profile whose shape is very similar to that of a stable Bonnor-Ebert sphere. Using SEST we also obtained a C18O spectrum toward the center of the cloud. The CO observation indicates that the globule is a gravitationally bound object. Analysis of the CO line profile reveals significant non-thermal gas motions likely due to turbulence. As a whole the globule may be evolving to a global state of quasi-static dynamical equilibrium in which thermal and turbulent pressure balance gravity.Comment: Contains 21 pages and 5 figures. Due to space limitations in astroph, figures 1, 3 & 5 are submitted as separate .jpg files. Full resolution figures and text can be downloaded from http://cfa-www.harvard.edu/~clada/preprints.htm

Between overt and covert research: concealment and disclosure in an ethnographic study of commercial hospitality

This article examines the ways in which problems of concealment emerged in an ethnographic study of a suburban bar and considers how disclosure of the research aims, the recruitment of informants, and elicitation of information was negotiated throughout the fieldwork. The case study demonstrates how the social context and the relationships with specific informants determined overtness or covertness in the research. It is argued that the existing literature on covert research and covert methods provides an inappropriate frame of reference with which to understand concealment in fieldwork. The article illustrates why concealment is sometimes necessary, and often unavoidable, and concludes that the criticisms leveled against covert methods should not stop the fieldworker from engaging in research that involves covertness