Composite reinforced propellant tanks

Design studies involving weight and cost were carried out for several structural concepts applicable to space shuttle disposable tankage. An effective design, a honeycomb stabilized pressure vessel, was chosen. A test model was designed and fabricated

Crosstalk between Integrin αvβ3 and Estrogen Receptor-α Is Involved in Thyroid Hormone-Induced Proliferation in Human Lung Carcinoma Cells

A cell surface receptor for thyroid hormone that activates extracellular regulated kinase (ERK) 1/2 has been identified on integrin αvβ3. We have examined the actions of thyroid hormone initiated at the integrin on human NCI-H522 non-small cell lung carcinoma and NCI-H510A small cell lung cancer cells. At a physiologic total hormone concentration (10−7 M), T4 significantly increased proliferating cell nuclear antigen (PCNA) abundance in these cell lines, as did 3, 5, 3′-triiodo-L-thyronine (T3) at a supraphysiologic concentration. Neutralizing antibody to integrin αvβ3 and an integrin-binding Arg-Gly-Asp (RGD) peptide blocked thyroid hormone-induced PCNA expression. Tetraiodothyroacetic acid (tetrac) lacks thyroid hormone function but inhibits binding of T4 and T3 to the integrin receptor; tetrac eliminated thyroid hormone-induced lung cancer cell proliferation and ERK1/2 activation. In these estrogen receptor-α (ERα)-positive lung cancer cells, thyroid hormone (T4>T3) caused phosphorylation of ERα; the specific ERα antagonist ICI 182,780 blocked T4-induced, but not T3-induced ERK1/2 activation, as well as ERα phosphorylation, proliferating-cell nuclear antigen (PCNA) expression and hormone-dependent thymidine uptake by tumor cells. Thus, in ERα-positive human lung cancer cells, the proliferative action of thyroid hormone initiated at the plasma membrane is at least in part mediated by ERα. In summary, thyroid hormone may be one of several endogenous factors capable of supporting proliferation of lung cancer cells. Activity as an inhibitor of lung cancer cell proliferation induced at the integrin receptor makes tetrac a novel anti-proliferative agent

Tracing river chemistry in space and time : dissolved inorganic constituents of the Fraser River, Canada

Author Posting. © The Author(s), 2013. This is the author's version of the work. It is posted here by permission of Elsevier for personal use, not for redistribution. The definitive version was published in Geochimica et Cosmochimica Acta 124 (2014): 283-308, doi:10.1016/j.gca.2013.09.006.The Fraser River basin in southwestern Canada bears unique geologic and climatic features which make it an ideal setting for investigating the origins, transformations and delivery to the coast of dissolved riverine loads under relatively pristine conditions. We present results from sampling campaigns over three years which demonstrate the lithologic and hydrologic controls on fluxes and isotope compositions of major dissolved inorganic runoff constituents (dissolved nutrients, major and trace elements, 87Sr/86Sr, δD). A time series record near the Fraser mouth allows us to generate new estimates of discharge-weighted concentrations and fluxes, and an overall chemical weathering rate of 32 t km-2 y-1. The seasonal variations in dissolved inorganic species are driven by changes in hydrology, which vary in timing across the basin. The time series record of dissolved 87Sr/86Sr is of particular interest, as a consistent shift between higher (“more radiogenic”) values during spring and summer and less radiogenic values in fall and winter demonstrates the seasonal variability in source contributions throughout the basin. This seasonal shift is also quite large (0.709 – 0.714), with a discharge-weighted annual average of 0.7120 (2 s.d. = 0.0003). We present a mixing model which predicts the seasonal evolution of dissolved 87Sr/86Sr based on tributary compositions and water discharge. This model highlights the importance of chemical weathering fluxes from the old sedimentary bedrock of headwater drainage regions, despite their relatively small contribution to the total water flux.This work was supported by the WHOI Academic Programs Office and MIT PAOC Houghton Fund to BMV, a WHOI Arctic Research Initiative grant to ZAW, NSF-ETBC grant OCE-0851015 to BPE and TIE, and NSF grant EAR-1226818 to BPE

Tetraiodothyroacetic acid (Tetrac) and nanoparticulate tetrac arrest growth of medullary carcinoma of the thyroid

Context: Tetraiodothyroacetic acid (tetrac) blocks angiogenic and tumor cell proliferation actions of thyroid hormone initiated at the cell surface hormone receptor on integrin alpha v beta 3. Tetrac also inhibits angiogenesis initiated by vascular endothelial growth factor and basic fibroblast growth factor. Objective: We tested antiangiogenic and antiproliferative efficacy of tetrac and tetrac nanoparticles (tetrac NP) against human medullary thyroid carcinoma (h-MTC) implants in the chick chorioallantoic membrane (CAM) and h-MTC xenografts in the nude mouse. Design: h-MTCcells were implanted in the CAM model (n = 8 per group); effects of tetrac and tetrac NP at 1 mu g/CAM were determined on tumor angiogenesis and tumor growth after 8 d. h-MTC cells were also implanted sc in nude mice (n = 6 animals per group), and actions on established tumor growth of unmodified tetrac and tetrac NP ip were determined. Results: In the CAM, tetrac and tetrac NP inhibited tumor growth and tumor-associated angiogenesis. In the nude mouse xenograft model, established 450-500 mm(3) h-MTC tumors were reduced in size over 21 d by both tetrac formulations to less than the initial cell mass (100 mm(3)). Tumor tissue hemoglobin content of xenografts decreased by 66% over the course of administration of each drug. RNA microarray and quantitative real-time PCR of tumor cell mRNAs revealed that both tetrac formulations significantly induced antiangiogenic thrombospondin 1 and apoptosis activator gene expression. Conclusions: Acting via a cell surface receptor, tetrac and tetrac NP inhibit growth of h-MTC cells and associated angiogenesis in CAM and mouse xenograft models.Charitable Leadership Foundation/Medical Technology Acceleration ProgramPharmaceutical Research Institute of Albany College of Pharmac

Uncertainty sources for measurable ocean carbonate chemistry variables

21 pages, 3 figures, 2 tables, supporting information https://doi.org/10.1002/lno.12477.-- Data availability statement: The GLODAPv2.2022 data product is freely available at the project website https://glodap.info/. The metadata data product is released as a supplementary .xlsx and .csv file with this submissionThe ocean carbonate system is critical to monitor because it plays a major role in regulating Earth's climate and marine ecosystems. It is monitored using a variety of measurements, and it is commonly understood that all components of seawater carbonate chemistry can be calculated when at least two carbonate system variables are measured. However, several recent studies have highlighted systematic discrepancies between calculated and directly measured carbonate chemistry variables and these discrepancies have large implications for efforts to measure and quantify the changing ocean carbon cycle. Given this, the Ocean Carbonate System Intercomparison Forum (OCSIF) was formed as a working group through the Ocean Carbon and Biogeochemistry program to coordinate and recommend research to quantify and/or reduce uncertainties and disagreements in measurable seawater carbonate system measurements and calculations, identify unknown or overlooked sources of these uncertainties, and provide recommendations for making progress on community efforts despite these uncertainties. With this paper we aim to (1) summarize recent progress toward quantifying and reducing carbonate system uncertainties; (2) advocate for research to further reduce and better quantify carbonate system measurement uncertainties; (3) present a small amount of new data, metadata, and analysis related to uncertainties in carbonate system measurements; and (4) restate and explain the rationales behind several OCSIF recommendations. We focus on open ocean carbonate chemistry, and caution that the considerations we discuss become further complicated in coastal, estuarine, and sedimentary environmentsThe Ocean Carbonate System Intercomparison Forum (OCSIF, https://www.us-ocb.org/ocean-carbonate-system-intercomparison-forum/) is a working group of subject matter experts that was established in 2019 with support from the Ocean Carbon and Biogeochemistry (OCB, us-ocb.org) Project Office, which receives funding from the National Science Foundation (NSF OCE-1850983) and the National Aeronautics and Space Administration (NASA NNX17AB17G). [...] B.R.C. thanks the Global Ocean Monitoring and Observing program of NOAA for funding the Carbon Data Management and Synthesis Program (Fund Ref. 100007298, program officer: Kathy Tedesco) and thereby supporting his involvement in OCSIF activities, as well as the funding the NA21OAR4310251 award (program officer: Virginia Selz), which supported the development and update of Supplementary Data S1. J.D.S. thanks the Global Ocean Monitoring and Observing program of NOAA (Award NA21OAR4310251), NOAA PMEL, and the University of Washington CICOES. R.J.W. acknowledges the National Science Foundation Division of Ocean Sciences (Oceans and Atmosphere [CSIRO] Canberra, Australia) and the MIT mTerra Catalyst fund. K.M.S. thanks the William and Elsie Knight Endowed Fellowship Fund for Marine Science (University of South Florida College of Marine Science), NOAA AOML, and the University of Miami CIMAS. K.L.F. thanks the St. Petersburg Downtown Partnership Fellowship in Coastal Science (University of South Florida College of Marine Science). M.I.G.-I. acknowledges the “Severo Ochoa Centre of Excellence” accreditation (CEX2019-000928-S). A.J.F. was supported by NOAA PMEL. M.Á. thanks the IEO internal project RADPROF. W.-J.C., R.A.E., and X.L. thank NOAA's Ocean Acidification Program via award: NA17OAR0170332. W.-J.C. also thanks NSF for support (EPSCoR-1757353 and OCE-2123768UW). [...] This research was carried out in part under the auspices of the Cooperative Institute for Climate, Ocean, and Ecosystem Studies (CICOES) and the Cooperative Institute for Marine and Atmospheric Studies (CIMAS), cooperative agreements NA20OAR4320271 and NA20OAR4320472, respectivelyPeer reviewe

The Privileged Normalization of Marijuana Use – an Analysis of Canadian Newspaper Reporting, 1997–2007

The objective of this study was to systematically examine predominant themes within mainstream media reporting about marijuana use in Canada. To ascertain the themes present in major Canadian newspaper reports, a sample (N = 1999) of articles published between 1997 and 2007 was analyzed. Drawing from Manning’s theory of the symbolic framing of drug use within media, it is argued that a discourse of ‘privileged normalization’ informs portrayals of marijuana use and descriptions of the drug’s users. Privileged normalization implies that marijuana use can be acceptable for some people at particular times and places, while its use by those without power and status is routinely vilified and linked to deviant behavior. The privileged normalization of marijuana by the media has important health policy implications in light of continued debate regarding the merits of decriminalization or legalization and the need for public health and harm reduction approaches to illicit drug use

Mammalian sex determination—insights from humans and mice

Disorders of sex development (DSD) are congenital conditions in which the development of chromosomal, gonadal, or anatomical sex is atypical. Many of the genes required for gonad development have been identified by analysis of DSD patients. However, the use of knockout and transgenic mouse strains have contributed enormously to the study of gonad gene function and interactions within the development network. Although the genetic basis of mammalian sex determination and differentiation has advanced considerably in recent years, a majority of 46,XY gonadal dysgenesis patients still cannot be provided with an accurate diagnosis. Some of these unexplained DSD cases may be due to mutations in novel DSD genes or genomic rearrangements affecting regulatory regions that lead to atypical gene expression. Here, we review our current knowledge of mammalian sex determination drawing on insights from human DSD patients and mouse models

Dating North America’s oldest petroglyphs, Winnemucca Lake subbasin, Nevada

On the west side of the Winnemucca Lake subbasin, Nevada, distinctive deeply carved meter-scale petroglyphs are closely spaced, forming panels on boulder-sized surfaces of a partially collapsed tufa mound. The large, complex motifs at this side are formed by deeply carved lines and cupules. A carbonate crust deposited between 10 200 and 9800 calibrated years B.P. (ka) coats petroglyphs at the base of the mound between elevations of 1202 and 1206 m. Petroglyphs above the carbonate crust are carved into a branching form of carbonate that dates to 14.8 ka. Radiocarbon dates on a multiple-layered algal tufa on the east side of the basin, which formed at an elevation of 1205 m, as well as a sediment-core-based total inorganic carbon record for the period 17.0‒9.5 ka indicate that water level in the Winnemucca Lake subbasin was constrained by spill over the Emerson Pass Sill (1207 m) for most of the time between 12.9 + 0.3 and ≥9.2 ka. These and other data indicate that the lake in the Winnemucca Lake subbasin fell beneath its spill point between 14.8 and 13.2 ka and also between 11.3 and 10.5 ka (or between 11.5 and 11.1 ka), exposing the base of the collapsed tufa mound to petroglyph carving. The tufa-based 14C record supports decreased lake levels between 14.8‒13.2 ka and 11.3‒10.5 ka. Native American artifacts found in the Lahontan Basin date to the latter time interval. This does not rule out the possibility that petroglyph carving occurred between 14.8 and 13.2 ka when Pyramid Lake was relatively shallow and Winnemucca Lake had desiccated