Motor-Incompatibility of Facial Reactions : The influence of valence and stimulus content on voluntary facial reactions

Emotional cues facilitate motor responses that are associated with approach or avoidance. Previous research has shown that evaluative processing of positive and negative facial expression stimuli is also linked to motor schemata of facial muscles. To further investigate the influence of different types of emotional stimuli on facial reactions, we conducted a study with pictures of emotional facial expressions (KDEF) and scenes (IAPS). Healthy participants were asked to respond to the positive or negative facial expressions (KDEF) and scenes (IAPS) with specific facial muscles in a valence-congruent (stimulus valence matches muscle related valence) or a valence-incongruent condition (stimulus valence is contrary to muscle related valence). Additionally, they were asked to rate pictures in terms of valence and arousal. Muscular response latencies were recorded by an electromyogram. Overall, response latencies were shorter in response to facial expressions than to complex pictures of scenes. For both stimulus categories, response latencies with valence-compatible muscles were shorter compared to reactions with incompatible muscles. Moreover, correlations between picture ratings and facial muscle reactions for happy facial expressions as well as positive scenes reflect a direct relationship between perceived intensity of the subjective emotional experience and physiological responding. Results replicate and extend previous research, indicating that incompatibility effects are reliable across different stimulus types and are not limited to facial mimicry

Inhibition of return in fear of spiders: Discrepant eye movement and reaction time data

Inhibition of return (IOR) refers to a bias against returning the attention to a previously attended location. As a foraging facilitator it is thought to facilitate systematic visual search. With respect to neutral stimuli, this is generally thought to be adaptive, but when threatening stimuli appear in our environment, such a bias may be maladaptive. This experiment investigated the influence of phobia-related stimuli on the IOR effect using a discrimination task. A sample of 50 students (25 high, 25 low in spider fear) completed an IOR task including schematic representations of spiders or butterflies as targets. Eye movements were recorded and to assess discrimination among targets, participants indicated with button presses if targets were spiders or butterflies. Reaction time data did not reveal a significant IOR effect but a significant interaction of group and target; spider fearful participants were faster to respond to spider targets than to butterflies. Furthermore, eye-tracking data showed a robust IOR effect independent of stimulus category. These results offer a more comprehensive assessment of the motor and oculomotor factors involved in the IOR effect

Tracing the cryptic Sardic (Ordovician) metamorphism across Alpine Europe: the Krndija region in the Slavonian Mountains, Croatia

Results of a combined petrological, geochemical and geochronological study suggest that metasedimentary rock units in the Krndija region of the Slavonian Mountains, Croatia, were affected by at least three major tectonometamorphic imprints: during the Middle Ordovician (Sardic event), the early Carboniferous (Variscan event), and the Cretaceous (Alpine event). All three metamorphic phases are established by electron microprobe-based in-situ U–Th–Pb dating of monazite grains. The Sardic metamorphic event is additionally confirmed by a precise Lu–Hf garnet-whole-rock isochron age of 466.0 ± 2.3 Ma. Taken together, the data unveil a relatively large and well-preserved piece of the cryptic Sardic orogen in central Krndija, that we name the Kutjevo Zone. A Sardic subduction-related metamorphic event (ca. 540-580 ℃, 8–11 kbar) at ca. 466 Ma is manifested in the mineral paragenesis Ca-rich garnet plus rutile. A low degree of retrograde reequilibration suggests a subsequent fast exhumation. Low-Ca cores in some garnets and staurolite relics record a pre-HP metamorphic event that involves isobaric heating from 570 to 610 ℃ at ~ 7 kbar. We attribute this (so far undated) event to mid-crustal contact metamorphism caused by early Sardic magmatism. Southern parts of Krndija (the Gradište Zone) experienced an (additional?) clockwise PT evolution in Variscan times at ca. 350 Ma. Garnet formed with ilmenite during a PT increase from 580 ℃/5 kbar to 600 ℃/6 kbar and underwent later strong retrograde resorption. Slow Variscan exhumation resulted in andalusite formation at < 550 ℃/ < 3.8 kbar. Penetrative Alpine metamorphism was observed in low-grade phyllites in the north. The lithology and metamorphic history of the Kutjevo Zone is similar to what has been reported from the Sardic Strona-Ceneri Zone in the western Alps. Both areas expose metapelitic (metagreywacke) rocks with a pre-middle Ordovician formation age. These metasedimentary rocks are inter-layered with numerous small amphibolitic units as well as metagranitoids and were likely deposited along the active Gondwana margin, perhaps in a fore-arc position, prior to their subduction during the middle Ordovician. According to recent palaeogeographic reconstructions, both the Kutjevo Zone and the Strona-Ceneri Zone have once resided in an eastern sector of the northern Gondwana margin (i.e., in E-Armorica). We conclude that in the Middle Ordovician, important subduction activities took place in this E-Armorican segment of north Gondwana, which is today exposed in the Alps. The W-Armorican segment of north Gondwana (now exposed in the French, German, and Czech Variscides) had probably already mutated from a (Cadomian) subduction setting to an extensional (transtensional–transpressional) setting by the late Cambrian

Dynamic changes in the higher-level chromatin organization of specific sequences revealed by in situ hybridization to nuclear halos

A novel approach to study the higher level packaging of specific DNA sequences has been developed by coupling high-resolution fluorescence hybridization with biochemical fractionation to remove histones and distend DNA loops to form morphologically reproducible nuclear halos. Results demonstrate consistent differences in the organization of specific sequences, and further suggest a relationship to functional activity. Pulse-incorporated bromodeoxyuridine representing nascent replicating DNA localized with the base of the chromatin loops in discrete clustered patterns characteristic of intact cells, whereas at increasing chase times, the replicated DNA was consistently found further out on the extended region of the halo. Fluorescence hybridization to unique loci for four transcriptionally inactive sequences produced long strings of signal extending out onto the DNA halo or loop, whereas four transcriptionally active sequences remained tightly condensed as single spots within the residual nucleus. In contrast, in non-extracted cells, all sequences studied typically remained condensed as single spots of fluorescence signal. Interestingly, two transcriptionally active, tandemly repeated gene clusters exhibited strikingly different packaging by this assay. Analysis of specific genes in single cells during the cell cycle revealed changes in packaging between S-phase and non S-phase cells, and further suggested a dramatic difference in the structural associations in mitotic and interphase chromatin. These results are consistent with and suggestive of a loop domain organization of chromatin packaging involving both stable and transient structural associations, and provide precedent for an approach whereby different biochemical fractionation methods may be used to unravel various aspects of the complex higher-level organization of the genome

Moduli-dependent Calabi-Yau and SU(3)-structure metrics from Machine Learning

We use machine learning to approximate Calabi-Yau and SU(3)-structure metrics, including for the first time complex structure moduli dependence. Our new methods furthermore improve existing numerical approximations in terms of accuracy and speed. Knowing these metrics has numerous applications, ranging from computations of crucial aspects of the effective field theory of string compactifications such as the canonical normalizations for Yukawa couplings, and the massive string spectrum which plays a crucial role in swampland conjectures, to mirror symmetry and the SYZ conjecture. In the case of SU(3) structure, our machine learning approach allows us to engineer metrics with certain torsion properties. Our methods are demonstrated for Calabi-Yau and SU(3)-structure manifolds based on a one-parameter family of quintic hypersurfaces in $\mathbb{P}^4.$Comment: 27+15 pages, 12 figures, 3 table

Integration of molecular typing results into tuberculosis surveillance in Germany-A pilot study.

An integrated molecular surveillance for tuberculosis (TB) improves the understanding of ongoing TB transmission by combining molecular typing and epidemiological data. However, the implementation of an integrated molecular surveillance for TB is complex and requires thoughtful consideration of feasibility, demand, public health benefits and legal issues. We aimed to pilot the integration of molecular typing results between 2008 and 2010 in the German Federal State of Baden-Württemberg (population 10.88 Million) as preparation for a nationwide implementation. Culture positive TB cases were typed by IS6110 DNA fingerprinting and results were integrated into routine notification data. Demographic and clinical characteristics of cases and clusters were described and new epidemiological links detected after integrating typing data were calculated. Furthermore, a cross-sectional survey was performed among local public health offices to evaluate their perception and experiences. Overall, typing results were available for 83% of notified culture positive TB cases, out of which 25% were clustered. Age <15 years (OR = 4.96, 95% CI: 1.69-14.55) and being born in Germany (OR = 2.01, 95% CI: 1.44-2.80) were associated with clustering. At cluster level, molecular typing information allowed the identification of previously unknown epidemiological links in 11% of the clusters. In 59% of the clusters it was not possible to identify any epidemiological link. Clusters extending over different counties were less likely to have epidemiological links identified among their cases (OR = 11.53, 95% CI: 3.48-98.23). The majority of local public health offices found molecular typing useful for their work. Our study illustrates the feasibility of integrating typing data into the German TB notification system and depicts its added public health value as complementary strategy in TB surveillance, especially to uncover transmission events among geographically separated TB patients. It also emphasizes that special efforts are required to strengthen the communication between local public health offices in different counties to enhance TB control