Modelling the injured spinal cord using 3-dimensional cell cultures; strategies for improving tissue engineered repair

Abstract not available

Feeding Behavior of Captive-Reared Juvenile Alligator Snapping Turtles (Macrochelys temminckii)

Feeding preference of Macrochelys temminckii (Alligator Snapping Turtle) is not well known. Juveniles reared with no prior exposure to natural prey were tested for innate prey (i.e., fish) preference and foraging ability for mussels in coarse and fine substrates. Alligator Snapping Turtles consumed fish non-selectively, except that they selected Lepomis macrochirus (Bluegill) over Gambusia affinis (Mosquitofish) in live-prey trials, and Lepomis cyanellus (Green Sunfish) over Notemigonus crysoleucas (Golden Shiners) in carrion trials. Juvenile Alligator Snapping Turtles were less active and less successful when foraging for a benthic prey species, Lampsilis siliquoidea (Fatmucket), in coarse substrate than they were when the mussels were in fine and no substrates. Juvenile Alligator Snapping Turtle preference for Bluegill in a controlled environment corresponds to predator and prey habitat associations but could also be influenced by prey (i.e., fish) behavior. Likewise, enhanced activity and prey encounters in fine substrate are consistent with observations of Alligator Snapping Turtle habitat use

A 3D <i>in vitro</i> model reveals differences in the astrocyte response elicited by potential stem cell therapies for CNS injury.

Aim: This study aimed to develop a 3D culture model to test the extent to which transplanted stem cells modulate astrocyte reactivity, where exacerbated glial cell activation could be detrimental to CNS repair success. Materials & methods: The reactivity of rat astrocytes to bone marrow mesenchymal stem cells, neural crest stem cells (NCSCs) and differentiated adipose-derived stem cells was assessed after 5 days. Schwann cells were used as a positive control. Results: NCSCs and differentiated Schwann cell-like adipose-derived stem cells did not increase astrocyte reactivity. Highly reactive responses to bone marrow mesenchymal stem cells and Schwann cells were equivalent. Conclusion: This approach can screen therapeutic cells prior to in vivo testing, allowing cells likely to trigger a substantial astrocyte response to be identified at an early stage. NCSCs and differentiated Schwann cell-like adipose-derived stem cells may be useful in treating CNS damage without increasing astrogliosis

THYMUS-DEPENDENT AREAS IN THE LYMPHOID ORGANS OF NEONATALLY THYMECTOMIZED MICE

Specific areas of lymphocyte depletion, termed thymus-dependent areas, have been delineated in neonatally thymectomized C3H/Bi and F1 (C57BL x C3H/Bi) mice. They occur within the lymphoid follicles of the spleen immediately surrounding the central arterioles, and constitute the mid and deep cortical zones of the lymph nodes. These depleted areas appear in healthy thymectomized mice as early as 3 wk after operation but, in mice which survive for more than 6 to 7 wk, the thymus-dependent areas are repopulated by rapidly dividing pyroninophilic cells, the majority of which are immature plasma cells. Syngeneic thymus cells, labeled in vitro with tritiated adenosine localize preferentially in the thymus-dependent areas after intravenous injection. Similarly labeled spleen cells also accumulate in these areas but, in addition, are distributed at the periphery of splenic follicles and in the outer cortical zone of the lymph nodes. Many more spleen than thymus cells enter the lymphoid tissues and the spleen appears to be the primary target. The apparent paradox that syngeneic thymus cells are less efficient than spleen cells in restoring neonatally thymectomized mice to normality is discussed in the light of these results and possible routes by which the migrating cells could enter the lymphoid tissues are considered. The origin of the plasma cells which repopulate the lymphocyte depleted areas is also discussed. It is concluded that the normal thymus produces cells which contribute directly to the migratory or circulatory lymphocyte population but that there also exists another source of supply for the plasma cell series. These two systems may function synergistically so that the thymus may control, directly or indirectly, the balance of cell populations within the body

Fate Of Abstracts Published In The Proceedings Of The First Annual Perinatal Society Of Australia And New Zealand Congress In 1997

Objectives: To examine the fate of research presented at the first annual Perinatal Society of Australia and New Zealand (PSANZ) Congress in 1997, by determining: the rate of publication in peer-reviewed biomedical journals; publication rate by discipline; journals in which work was published; concordance for aims, conclusions, authors and number of study subjects; and time from presentation to publication. Methods: A MEDLINE search was conducted for any publication in a peer-reviewed journal resulting from a publishable abstract from the proceedings of the first annual PSANZ Congress in 1997. Searching was completed 42 months postcongress. The concordance of aims, conclusions, authors and number of subjects between abstract and published paper was determined. Results: There were 172 publishable abstracts in the proceedings of the PSANZ Congress in 1997, and 78 (45%) were published as 83 articles. Basic sciences had the highest publication rate (67%) and midwifery the lowest (20%). Articles were published in 41 journals, with one-third of the articles in three paediatric journals. There was a match with aims in 75%, and with conclusions in 65%. There were 47/77 with the same number of subjects, 20/77 with more and 10/77 with fewer. There were 22 articles with one author added, 12 had more than one author added, 11 had one author removed and five had more than one author removed. Median time-to-publication was 18 months (interquartile range 9-26 months). Conclusions: A publication rate of 45% is comparable to other conferences. Basic science and neonatology had the highest publication rates. There were considerable differences between abstract and published article in terms of aims, conclusions, number of subjects and authors

Astrocyte alignment in 3D collagen gels increases neurite outgrowth; implications for improving spinal cord repair

A major impediment to tissue engineered repair of CNS damage is the glial scar that forms around implanted graft devices and creates an inhibitory environment for axon growth out of the repair site 1. The glial scar is composed of a 3-dimensional (3D) meshwork of astrocytes which become reactive in response to damage stimuli. Previous studies have shown that longitudinal alignment of astrocytes growing in monolayer is sufficient to direct and enhance the growth of neurites over their surface 2,3. The aim of this work therefore was to develop a 3D culture system in which the effect of astrocyte alignment on neurite growth could be modelled in a spatially relevant environment

The Wisconsin H-Alpha Mapper Northern Sky Survey

The Wisconsin H-Alpha Mapper (WHAM) has surveyed the distribution and kinematics of ionized gas in the Galaxy above declination -30 degrees. The WHAM Northern Sky Survey (WHAM-NSS) has an angular resolution of one degree and provides the first absolutely-calibrated, kinematically-resolved map of the H-Alpha emission from the Warm Ionized Medium (WIM) within ~ +/-100 km/s of the Local Standard of Rest. Leveraging WHAM's 12 km/s spectral resolution, we have modeled and removed atmospheric emission and zodiacal absorption features from each of the 37,565 spectra. The resulting H-Alpha profiles reveal ionized gas detected in nearly every direction on the sky with a sensitivity of 0.15 R (3 sigma). Complex distributions of ionized gas are revealed in the nearby spiral arms up to 1-2 kpc away from the Galactic plane. Toward the inner Galaxy, the WHAM-NSS provides information about the WIM out to the tangent point down to a few degrees from the plane. Ionized gas is also detected toward many intermediate velocity clouds at high latitudes. Several new H II regions are revealed around early B-stars and evolved stellar cores (sdB/O). This work presents the details of the instrument, the survey, and the data reduction techniques. The WHAM-NSS is also presented and analyzed for its gross properties. Finally, some general conclusions are presented about the nature of the WIM as revealed by the WHAM-NSS.Comment: 42 pages, 14 figures (Fig 6-9 & 14 are full color); accepted for publication in 2003, ApJ, 149; Original quality figures (as well as data for the survey) are available at http://www.astro.wisc.edu/wham

Development for change.

Lifelong Learning Networks are concerned with bringing about change in the practices and the provision of higher education to address the issue of low progression through vocational, applied and work-based routes. MOVE Lifelong Learning Network has sought to create a step change in lifelong learning and maximise the vocational progression opportunities for individuals within the MOVE learner constituency in the East of England. This includes: those with vocational or applied qualifications at level three those qualifying through work-based learning routes return to study learners seeking entry into vocational programmes either directly or through Access to Higher Education provision This document describes how MOVE has strategically employed the use of development funding to generate a critical mass of curriculum and other development activity within the region to bring about the step change in provision that is required to meet the needs of these learners. The aim is to demonstrate how targeted funding can stimulate significant innovation in the way in which higher education is delivered, in the type and range of provision that is available and in the practices employed to promote and support progression to higher education. In addition, the document describes how such changes in provision can contribute to a more employer led approach to curriculum development. The text also includes a range of project case study examples to illustrate the qualitative impact of the development activity supported by MOVE