Calcitonin receptor-like receptor is expressed on gastrointestinal immune cells

Background/Aims: Pharmacological and morphological studies suggest that the gut mucosal immune system and local neuropeptide-containing neurones interact. We aimed to determine whether gut immune cells are targets for calcitonin gene-related peptide (CGRP), which has potent immune regulatory properties. Methods: Using density gradient centrifugation, rat lamina propria mononuclear cells (LP-MNCs) and intra-epithelial lymphocytes (IELs) were isolated. RT-PCR was employed for the detection of mRNA of rat calcitonin receptor-like receptor (CRLR), which is considered to represent the pharmacologically defined CGRP receptor-1 subtype, as well as mRNA of the receptor activity-modifying proteins, which are essential for CRLR function and determine ligand specificity. A radioreceptor assay was employed for the detection of specific CGRP binding sites. Results: RT-PCR and DNA sequencing showed that LP-MNCs and IELs express CRLR. Incubation of isolated LP-MNCs with radiolabelled alphaCGRP revealed the existence of specific binding sites for CGRP. Conclusion: These novel data indicate that mucosal immune cells of the rat gut are a target for CGRP and provide significant evidence that CGRP functions as an immune regulator in the gut mucosa. Copyright (C) 2002 S. Karger AG, Basel

Group 13 Decamethylmetallocenium Cations

Salts containing the decamethylmetallocenium cations, [( C5Me5) M-2](+) ( or Cp*M-2(+)) of the group 13 "metals" B, Al and Ga have been prepared using a variety of synthetic routes. Precursor molecules of the type Cp*2MX ( X = Cl, Br, Me) exhibit structural features that vary significantly depending on the size and electronegativity of the central atom. While salt metathesis, halide abstraction and methanide abstraction methods represent viable routes for the preparation of salts of Cp*B-2(+) and Cp*Al-2(+), acidolysis of a Cp* group from Cp*Ga-3 is the most reliable method for the synthesis of the analogous gallium cation. Gallocenium cations are less stable than either of the lighter congeneric cations since they prove to be susceptible to decomposition reactions involving the "back-transfer" of ligands from the counter anion. Density functional theory (DFT) calculations revealed that, whereas Cp*Ga-2(+) is predicted to adopt a molecular structure more similar to that of Cp*B-2(+), the electronic structure of the gallium cation bears a greater resemblance to that of Cp*Al-2(+).Chemistr

Integral representation of the linear Boltzmann operator for granular gas dynamics with applications

We investigate the properties of the collision operator associated to the linear Boltzmann equation for dissipative hard-spheres arising in granular gas dynamics. We establish that, as in the case of non-dissipative interactions, the gain collision operator is an integral operator whose kernel is made explicit. One deduces from this result a complete picture of the spectrum of the collision operator in an Hilbert space setting, generalizing results from T. Carleman to granular gases. In the same way, we obtain from this integral representation of the gain operator that the semigroup in L^1(\R \times \R,\d \x \otimes \d\v) associated to the linear Boltzmann equation for dissipative hard spheres is honest generalizing known results from the first author.Comment: 19 pages, to appear in Journal of Statistical Physic

Ultrasonic studies of the magnetic phase transition in MnSi

Measurements of the sound velocities in a single crystal of MnSi were performed in the temperature range 4-150 K. Elastic constants, controlling propagation of longitudinal waves reveal significant softening at a temperature of about 29.6 K and small discontinuities at $\sim$28.8 K, which corresponds to the magnetic phase transition in MnSi. In contrast the shear elastic moduli do not show any softening at all, reacting only to the small volume deformation caused by the magneto-volume effect. The current ultrasonic study exposes an important fact that the magnetic phase transition in MnSi, occurring at 28.8 K, is just a minor feature of the global transformation marked by the rounded maxima or minima of heat capacity, thermal expansion coefficient, sound velocities and absorption, and the temperature derivative of resistivity.Comment: 4 pages, 4 figure

Calibration biases in measurements of weak lensing

As recently shown by Viola et al., the common (KSB) method for measuring weak gravitational shear creates a non-linear relation between the measured and the true shear of objects. We investigate here what effect such a non-linear calibration relation may have on cosmological parameter estimates from weak lensing if a simpler, linear calibration relation is assumed. We show that the non-linear relation introduces a bias in the shear-correlation amplitude and thus a bias in the cosmological parameters Omega_matter and sigma_8. Its direction and magnitude depends on whether the point-spread function is narrow or wide compared to the galaxy images from which the shear is measured. Substantial over- or underestimates of the cosmological parameters are equally possible, depending also on the variant of the KSB method. Our results show that for trustable cosmological-parameter estimates from measurements of weak lensing, one must verify that the method employed is free from ellipticity-dependent biases or monitor that the calibration relation inferred from simulations is applicable to the survey at hand.Comment: 5 pages, 3 figures, submitted to A&