Screening for Resistance to Plum Pox Virus in Some Local Turkish Apricot Cultivars and Their Crosses by Molecular Markers

2nd International Symposium on Plum Pox Virus -- SEP 03-06, 2013 -- Olomouc, CZECH REPUBLICWOS: 000358036400017Turkey is the most important producer and exporter country of apricot, Prunus armeniaca. Production of apricots for fresh market relies on foreign cultivars grown on Mediterranean and Aegean regions while Malatya is the most important region for production of dry apricots based on local cultivars. Plum pox virus (PPV) in Turkey has been known since 1968, but it was not widespread until recent years. Malatya region has been free of sharka disease so far, but the disease has already been reported from many different provinces since 2006. Because of that, introgression of resistance to PPV in the local cultivars with good pomological characteristics became an important objective for the apricot crop. In the current breeding program, obtaining new cultivars resistant to PPV, selection of resistant seedlings by using molecular markers linked to PPV resistance was aimed at. Nineteen local apricot genitors and progenies obtained from the crosses between the PPV resistant cultivar 'Stark Early Orange' (SEO), 'Harcot' and local cultivars such as 'Hacihaliloglu', 'Kabaasi', 'Hasanbey', 'Cologlu', 'Adilcevaz5', 'Sekerpare', 'MahmudunErigi', 'Soganci' and 'Cataloglu' were screened with markers. The markers PGS1.21 and PGS2.23 co-segregating with resistance to PPV were used to screen a total of 189 apricot progenies. None of the local genitors had alleles linked to PPV resistance. Among the progenies screened, 15 seedlings from 'Sekerpare' by SEO, 12 from 'Adilcevaz5' by SEO, 7 from 'Hacihaliloglu' by SEO, 9 from 'Kabaasi' by SEO, 5 from 'Cologlu' by SEO, 9 from 'Cataloglu' by SEO, 4 from 'Hasanbey' by SEO, and 1 from 'MahmudunErigi' by SEO and none of the 'Harcot' by 'Soganci' presented resistant alleles and were selected for further studies.Int Soc Horticultural Sc

Investigation of resistance of apricot progeny to Plum pox virus through molecular markers

3rd International Symposium on Plum Pox Virus -- MAY 09-13, 2016 -- Antalya, TURKEYWOS: 000428232700005Plum pox virus (PPV) is the causal agent of sharka disease, which is mainly destructive on apricot (Prunus armeniaca L.), plum (Prunus domestica L.) and peach (Prunus persica L.). There are no efficient control methods except using PPV-free propagating materials and planting PPV-resistant or at least less-susceptible rootstocks. Therefore, lots of studies have been conducted in recent years on breeding of PPV-resistant plants. The objective of this study was the introduction and development of marker-assisted selection (MAS) for PPV resistance in F-1 and F-2 progenies of some Turkish apricot cultivars. Local apricot cultivars 'Adilcevaz 5', 'cologlu', 'Sekerpare' and 'cataloglul crossed with PPV-resistant 'Stark Early Orange' (SEO) were screened with molecular markers PGS1.21 and PGS2.23 co segregating with resistance to PPV in 2011. Of all combinations, seven of 20 progeny obtained from SEO x 12 of 34 progeny obtained from SEO x 'Adilcevaz 5, five of 10 progeny obtained from SEO x 'coloklu, 15 of 37 progeny obtained from SEO x `Sekerpare' and eight of 33 progeny obtained from SEO x 'cataloglu' exhibited resistant alleles.Int Soc Horticultural Sc

Genetic diversity and molecular epidemiology of the T strain of <em>Plum pox virus</em>

Very limited information is available on the origin, diversity and evolution of Plum pox virus (PPV) “Turkey” (T) strain. Phylogenetic analyses based on partial sequences of 421 isolates and complete genome sequences of 57 isolates, representing the geographical distribution of PPV‐T in Turkey revealed the existence of several monophyletic and, in some cases, geographically limited groups within the PPV‐T strain (Ankara‐Konya1‐Kayseri, Ankara‐Balkan, Istanbul, Konya2 and Balkan). PPV‐T diversity (0.018%) was found to be greater than that of PPV strains D and Rec but lower than the of the M strain when including the newly described and divergent M‐Istanbul isolates, suggesting a long evolutionary history for PPV‐T. The European part of Turkey in the Balkan, which is close to Bulgaria where PPV was identified for the first time, appears as a likely origin center for PPV‐T isolates. A colonization of various parts of Turkey by diverse isolates from that region, followed by secondary local spread is the most likely scenario for the diffusion of PPV‐T in Turkey

cell lung cancer in Turkey

Aim: The early diagnosis and treatment of lung cancer are important for the prognosis of patients with lung cancer. This study was undertaken to investigate patient and doctor delays in the diagnosis and treatment of NSCLC and the factors affecting these delays.Materials and methods: A total of 1016 patients, including 926 (91.1%) males and 90 (8.9%) females with a mean age of 61.5 +/- 10.1 years, were enrolled prospectively in this study between May 2010 and May 2011 from 17 sites in various Turkish provinces.Results: The patient delay was found to be 49.9 +/- 96.9 days, doctor delay was found to be 87.7 +/- 99.6 days, and total delay was found to be 131.3 +/- 135.2 days. The referral delay was found to be 61.6 +/- 127.2 days, diagnostic delay was found to be 20.4 +/- 44.5 days, and treatment delay was found to be 24.4 +/- 54.9 days. When the major factors responsible for these delays were examined, patient delay was found to be more frequent in workers, while referral delay was found to be more frequent in patients living in villages (p < 0.05). We determined that referral delay, doctor delay, and total delay increased as the number of doctors who were consulted by patients increased (p < 0.05). Additionally, we determined that diagnostic and treatment delays were more frequent at the early tumour stages in NSCLC patients (p < 0.05).Discussion: The extended length of patient delay underscores the necessity of educating people about lung cancer. To decrease doctor delay, education is a crucial first step. Additionally, to further reduce the diagnostic and treatment delays of chest specialists, multidisciplinary management and algorithms must be used regularly. (C) 2015 Elsevier Ltd. All rights reserved