Prospective life cycle inventory datasets for conventional and hybrid-electric aircraft technologies

Hybrid-electric aircraft represent a promising solution for the urgent need to decarbonize short-haul flights and bolster aviation sustainability. Nevertheless, the realization of hybrid-electric aircraft demands rigorous environmental impact analysis, given the substantial investments, time, and research required for technology development. This study offers a comprehensive life cycle inventory spanning the years 2030, 2040, and 2050 for both conventional and hybrid-electric aircraft configurations. Our inventory datasets are meticulously constructed through a systematic approach, ensuring data harmonization by drawing upon scientific literature, industry expertise, and primary data sources. This extensive dataset encompasses all pertinent systems necessary to model the environmental footprint of flights covering distances ranging from 200 to 600 nautical miles, utilizing a 50-passenger aircraft with the ATR42 as a reference model. Additionally, we furnish supplemental data for end-of-life considerations and uncertainty analysis. The systems under examination include the airframe, powertrain, power electronics and drives, batteries, fuel cells, hydrogen onboard storage, airport infrastructure, and battery charging stations. Notably, the carbon footprint of conventional aircraft aligns with data from the ecoinvent v3.8 database; however, our provided datasets are more than tenfold more detailed and incorporate a forward-looking perspective. These meticulously curated life cycle inventories can be amalgamated to simulate the potential environmental ramifications of conventional aircraft powered by kerosene or alternative aviation fuels, hybrid-electric aircraft utilizing battery technology, and hybrid-electric aircraft employing hydrogen as a fuel in conjunction with batteries. In this context, our findings play a pivotal role in nurturing the development of technology roadmaps that prioritize environmental sustainability within the realm of regional aviation

Gender bias and the journal of roman studies

Reflecting on present unease about structural biases in the discipline, and aiming to offer a data-rich response to some recent criticisms of this Journal, the Editorial Board has undertaken a study of the representation of female scholars in the Journal of Roman Studies. To that end, we have gathered data on publications, submissions and JRS Editorial Board membership for the past fifteen years, from Volume 95 (2005) through to the present volume, Volume 109 (2019). The data are set out in the final section (VII), following a brief review of the main results. Our goal here is neither to present a definitive analysis, nor to offer a commentary on the underlying causes of the patterns revealed (on which we expect much fruitful discussion elsewhere). Rather, the JRS Editorial Board aims to make key data available both to inform a much wider debate within the profession as a whole and, importantly, to inform this Journal’s policies, procedures and active outreach. The Board is also acutely aware that any analysis of gender bias needs to be framed carefully — both by an awareness that there are other under-represented groups in the discipline (on which our data in their current form would regrettably only offer a most imperfect picture), and by a sensitivity to the limitations of a conception of gender as a simple binary

Immortalization of bovine dental papilla cells with simian virus 40 large t antigen

Primary cultures of dental papilla-derived cells have a limited lifespan in vitro and can be maintained only up to passage 7-9 before showing senescence, but in vitro investigations often require a large number of cells showing phenotypic characteristics of the original tissue. To overcome this shortcoming, second-passage cells established from calf molar tooth germs by enzymatic pretreatment of the dental papilla were transfected by electroporation with pSV3neo, coding for the oncogene simian virus 40 large t antigen and a neomycin-resistance gene. Under selection by G418 (neomycin), four cell clones were isolated by single cell dilution at passage 15. Integration of simian virus 40 large t antigen and expression of the gene products were determined in cell clones by polymerase chain reaction (PCR) and immunohistochemistry. Four transfected cell lines (clones B, C, D and no. 12) were maintained in culture for over 1.5 years. For cell characterization, gene expression of procollagen alpha 1 (I) and osteocalcin was evaluated by reverse transcriptase (RT)-PCR with cDNA obtained from the established cell lines at passage 20. Expression of collagen type I, osteocalcin and dentine phosphoprotein was evaluated immunohistochemically at passage 20 and after 1.5 years of continuous cell culture. Gene expression and the expression of mineralized tissue-specific proteins was demonstrated with RT-PCR and immunohistochemistry within all four immortalized cell lines. Expression of dentine phosphoprotein was observed in three simian virus 40 large t antigen-transfected cell lines, suggesting the immortalization of odontoblast-like cells in vitro. Thus, transfection of bovine dental papilla-derived cells resulted in immortal cell lines exhibiting phenotypic characteristics of the original tissue. (C) 2000 Elsevier Science Ltd. All rights reserved

Responses of L929 mouse fibroblasts, primary and immortalized bovine dental papilla-derived cell lines to dental resin components

Objective: The use of adequate target cells for cytotoxicity testing of dental restorative materials has often been experimentally assessed with respect to the clinical relevance of the test results. In the present study, the responses in primary bovine dental papilla-derived cells (pulp cells) were compared with those in transformed dental papilla-derived cell lines and L929 mouse fibroblasts after exposure to various dental resin compounds. Methods: Primary bovine dental papilla-derived cells (CPC), tCPC B (CPC cells transformed with SV40 T-antigen), tCPC E (CPC cells transformed with E6/E7 oncogen), and L929 mouse fibroblast cells were exposed to various compounds of dental resin materials for 24 h, and cytotoxicity was determined using the MTT assay. Bis-GMA, UDMA, 1,6 hexane diol dimethacrylate (HDDM), TEGDMA, HEMA, NIMA, camphorquinone (CQ), bisphenol A (BPA), and glycidyl methacrylate (GMA) were tested. Concentrations leading to 50% cell survival (TC50 values) were calculated from fitted dose-response curves. Results: The simple ranking of the cytotoxic effects of the dental resin compounds in the four cell types was identical, and TC50 values determined in L929 cells here were consistent with findings by other authors using continuous cell lines. However, the concentrations of the resin compounds necessary for eliciting cytotoxic responses in the various cells were clearly different. The analyses of TC50 values of the resin compounds revealed a linear correlation between cell lines, and the overall sensitivities increased as follows: CPC = tCPC B < tCPC E < L929. Significance: The low sensitivities of primary cells and transformed tCPC B cells compared with the continuous L929 cell line and the transformed tCPC E cells indicates the presence of specific structural and functional properties relevant in vivo. The differences between the transformed tCPC B and tCPC E cells may indicate modifications of cellular functions caused by the different transformation processes. (C) 2002 Academy of Dental Materials. Published by Elsevier Science Ltd. All rights reserved