Comparison of biotin binding protein of pregnant rat serum with rat serum albumin

The purified biotin binding protein of pregnant rat serum was shown to be immunologically similar to rat serum albumin as assessed by a sensitive radioimmunoassay. In radioimmunoassay for rat biotin binding protein, the binding of [125I] rat biotin binding protein to anti-chicken egg yolk biotin binding protein antibodies was displaced by both rat serum (10-100 nl) and purified rat serum albumin (0.1-10 ng). Similarly, in radioimmunoassay for rat serum albumin the binding of [125I] rat serum albumin to either anti-rat serum albumin antibodies or anti-chicken egg yolk biotin binding protein antibodies was displaced by unlabelled rat biotin binding protein at comparable concentration range (0.5-10 ng). Significant fractions of radioiodinated rat biotin binding protein and rat serum albumin bound to antibodies to chicken egg yolk biotin binding protein. In immature rats, the circulating half-lives of rat biotin binding protein and rat serum albumin were determined to be 12 and 17 h respectively. The rat biotin binding protein and rat serum albumin were analysed by techniques that exploit their physicochemical properties. They displayed similar electrophoretic mobilities in alkaline as well as denaturing sodium dodecyl sulphate-polyacrylamide gels. However, in nonequilibrium pH gradient polyacrylamide gel electrophoresis, they resolved clearly. In two-dimensional tryptic peptide map analysis, the two proteins showed similarities as well as significant differences in the relative distribution patterns of their iodopeptides. These results showed that the primary structure of rat biotin binding protein and rat serum albumin were different in finer details despite the fact that they shared significant immunological cross-reactivity

Studies on the Effects of Bioprocess Parameters and Kinetics of Rhamnolipid Production by P. aeruginosa NITT 6L

Biosurfactants are gaining popularity in recent times due to lower toxicity, biodegradability, environmental compatibility and activity in extreme conditions. An air isolate was isolated previously for biosurfactant production in our laboratory, and characterized and named as P. aeruginosa NITT 6L. The biosurfactant thus produced was characterized to be surface-active rhamnolipid. This paper presents the study of the influence of various bioprocess parameters such as agitation, aeration and inoculum volume on rhamnolipid production by the isolate. Kinetics of rhamnolipid production in optimized media and process conditions were investigated. The rhamnolipid production was found to be increased after nitrogen depletion during stationary phase. The maximum rhamnolipid concentration of about 7.65 g L–1 was achieved after 96 h. Logistic model was found to be satisfactory in fitting the microbial growth. Emulsification activity of the crude rhamnolipid extract with different hydrocarbons was studied. The crude extract of rhamnolipid reduced the surface tension of water from 71.4 to 27.5 mN m–1, and CMC was about 11 mg L–1. Also, the usefulness of the extracted rhamnolipid produced under optimal conditions was investigated for remediation of crude oil contaminated soil. Soil washing with 0.3 % rhamnolipid removed about 71 % of crude oil present in sand samples within 24 h

Combined Effect of High Intensity Intermittent Training and Weight Training on Aerobic Capacity Anaerobic Capacity and Fatigue Index of Male Handball Players

The purpose of the present study is to assess the combine effect of high intensity training and weight training on aerobic capacity, anaerobic capacity and fatigue index of male handball players. Thirty six male handball players were randomly selected from Sports Authority of India, Sports Training Centre (STC), Sarurnagar, Hyderabad, Andhra Pradesh. These subjects were classified into two groups, namely Group I - Combined training (12) and Group II - Control group (12). The Group I underwent high intensity intermittent training and weight training and Group II acted as control. The selected players had regular practice and took part in competitions. The selected subjects average age, height and weight were 21.55 ± 2.15 years, 170.05 ± 6.98 cm and 64.94 ± 8.50 kg respectively. In the present study we selected aerobic capacity, anaerobic capacity and fatigue index as criterion variable which was assessed by multi stage fitness test and running based anaerobic sprint test. High intensity intermittent training and weight training was performed 4 session per weeks (2 session of high intensity intermittent training and 2 session weight training) for 8 weeks respectively. All the selected subjects anaerobic capacity, fatigue index and aerobic capacity was measured before and after 8 weeks of training to respective groups. ANCOVA was applied to know the changes based on training. The result of the study showed that 8 weeks of combined training significantly improved aerobic capacity (F (1,21) = 104.84, p < 0.05), anaerobic capacity (F (1,21) = 20.33,p < 0.05) and fatigue index (F (1,21) = 21.35, p < 0.05) of the handball players after adjusting the pre test. It is concluded that combined training is efficient enough to improve aerobic capacity, anaerobic capacity and fatigue index of male handball players

Bacillus cereus KLUVAA Mediated Biocement Production Using Hard Water and Urea

In the present study, a potential bacterial strain with maximum urease activity was isolated from urea-rich paddy field soil for biocement production. The bacterial isolate was screened using Christensen selective agar media and named as KLUVAA. This isolate was found to be tolerant up to 10 % urea. 16S rRNA sequencing analysis identified the isolate KLUVAA as Bacillus cereus. Biocement production was carried out using tap water with 431.7 mg L–1 of hardness as a natural source of calcium. Functional groups present in biocement were analysed using FT-IR spectrum. The morphology and elemental composition of the biocement was studied using SEM with EDS mapping and XRD analyses. Thermogravimetric analysis was used to study the thermal stability of the microbial biocement. Further, process parameters were optimized for enhancing the yield of biocement. This work is licensed under a Creative Commons Attribution 4.0 International License

Ultrasonic studies in aqueous solutions of uranyl nitrate

This article does not have an abstract

The fishery, biology and stock assessment of jew fish resources of India

Sciaenids are one of the major component of the demersal trawl The total catch of this resource during 1990-94 period was 1.50,142 t contributing 8.86% to the demersal catch of India. A number of species are found in different states of India. Of which biological and stock assessment studies were made on eleven important species. Crustaceans and fish appear to be the chief food in Juvenile and adult stage respectively. Most of the species have a protracted spawning season. Among all the species studied the largest asymptotic length was estimated for O.ruber from Tuticorin and the smallest for J. sina from Cochin. The highest Z of 7.59 was recorded for K. axillaris from Chennai and the lowest was for O. cuvierifrom Mumbai. The average exploitation rate (E) and the Lc/ Lao was 0.62 and 0.53 respectively. The present yield is 91.222 t and the MSY is 1.42,613 t for all the species taken together. The exploitation rate for almost all the stocks in the states appears to be more than the optimum leve

Major and minor fisheries harbours of India. 8. Fisherries harbours along the coasts of North Andhra Pradesh, Orissa and West Bengal

The present account gives information about the three major and five minor fisheries harbours along the coasts of north Andhra Pradesh, Orissa and West Benga

Both the Caspase CSP-1 and a Caspase-Independent Pathway Promote Programmed Cell Death in Parallel to the Canonical Pathway for Apoptosis in Caenorhabditis elegans

Caspases are cysteine proteases that can drive apoptosis in metazoans and have critical functions in the elimination of cells during development, the maintenance of tissue homeostasis, and responses to cellular damage. Although a growing body of research suggests that programmed cell death can occur in the absence of caspases, mammalian studies of caspase-independent apoptosis are confounded by the existence of at least seven caspase homologs that can function redundantly to promote cell death. Caspase-independent programmed cell death is also thought to occur in the invertebrate nematode Caenorhabditis elegans. The C. elegans genome contains four caspase genes (ced-3, csp-1, csp-2, and csp-3), of which only ced-3 has been demonstrated to promote apoptosis. Here, we show that CSP-1 is a pro-apoptotic caspase that promotes programmed cell death in a subset of cells fated to die during C. elegans embryogenesis. csp-1 is expressed robustly in late pachytene nuclei of the germline and is required maternally for its role in embryonic programmed cell deaths. Unlike CED-3, CSP-1 is not regulated by the APAF-1 homolog CED-4 or the BCL-2 homolog CED-9, revealing that csp-1 functions independently of the canonical genetic pathway for apoptosis. Previously we demonstrated that embryos lacking all four caspases can eliminate cells through an extrusion mechanism and that these cells are apoptotic. Extruded cells differ from cells that normally undergo programmed cell death not only by being extruded but also by not being engulfed by neighboring cells. In this study, we identify in csp-3; csp-1; csp-2 ced-3 quadruple mutants apoptotic cell corpses that fully resemble wild-type cell corpses: these caspase-deficient cell corpses are morphologically apoptotic, are not extruded, and are internalized by engulfing cells. We conclude that both caspase-dependent and caspase-independent pathways promote apoptotic programmed cell death and the phagocytosis of cell corpses in parallel to the canonical apoptosis pathway involving CED-3 activation.Howard Hughes Medical InstituteDamon Runyon Cancer Research FoundationCharles A. King Trus

Wnt addiction of genetically defined cancers reversed by PORCN inhibition

Enhanced sensitivity to Wnts is an emerging hallmark of a subset of cancers, defined in part by mutations regulating the abundance of their receptors. Whether these mutations identify a clinical opportunity is an important question. Inhibition of Wnt secretion by blocking an essential post-translational modification, palmitoleation, provides a useful therapeutic intervention. We developed a novel potent, orally available PORCN inhibitor, ETC-1922159 (henceforth called ETC-159) that blocks the secretion and activity of all Wnts. ETC-159 is remarkably effective in treating RSPO-translocation bearing colorectal cancer (CRC) patient-derived xenografts. This is the first example of effective targeted therapy for this subset of CRC. Consistent with a central role of Wnt signaling in regulation of gene expression, inhibition of PORCN in RSPO3-translocated cancers causes a marked remodeling of the transcriptome, with loss of cell cycle, stem cell and proliferation genes, and an increase in differentiation markers. Inhibition of Wnt signaling by PORCN inhibition holds promise as differentiation therapy in genetically defined human cancers