Antimicrobial resistance of commensal E. coli from the pork food chain in Germany

In the framework of the National Zoonoses Monitoring in Germany, E. coli from pigs and pork as well as from wild boar meat have been tested for their resistance to a panel of 14 antimicrobials using the broth microdilution method according to CLSI. The determined minimum inhibitory concentrations were evaluated based on the epidemiological cut-off values as provided by EUCAST

Pseudophakic corneal donor tissue in Descemet membrane endothelial keratoplasty (DMEK): implications for cornea banks and surgeons

Objective: Increasingly, cornea banks are recovering donor tissue from pseudophakic donors. Little is known about their suitability for Descemet membrane endothelial keratoplasty (DMEK) surgery in terms of endothelial cell density (ECD) and preparation failure. Methods and Analysis: We explored ECD during donor tissue preparation in 2076 grafts. Preparation failure was analysed in 1028 grafts used in DMEK surgery at our clinic. To monitor ECD and functional results, we matched 86 DMEK patients who received pseudophakic donor grafts with similar recipients of phakic donor grafts and followed them up for 36 months. Results: At recovery, mean ECD in pseudophakic donor grafts was 2193 cells/mm2 (SD 28.7) and 2364 cells/mm2 (SD 15.7) in phakic donor grafts (p<0.001). After cultivation, the difference increased as pseudophakic donor grafts lost 14% of ECD while phakic lost only 6% (p<0.001). At transplantation, mean ECD in pseudophakic donor grafts was 2272 cells/mm2 (SD 250) and 2370 cells/mm2 (SD 204) in phakic donor grafts (p<0.001). After transplantation, the difference in ECD increased as pseudophakic donor grafts lost 27.7% of ECD while phakic donor grafts lost only 13.3% (p<0.001). The risk of preparation failure in pseudophakic donor grafts was higher than in phakic donor grafts (OR 4.75, 95% CI 1.78 to 12.67, p=0.02). Visual acuity increased in both groups similarly. Conclusions: Pseudophakic donor grafts have a lower ECD, are more prone to endothelial cell loss during recovery and surgery and are associated with a higher risk of preparation failure. Cornea banks and surgeons should consider this in the planning of graft preparation and transplantation

The Perceived Value of Certification by Certified Perioperative Nurses

We conducted this study to determine the perceived value of certification in perioperative nursing. Following development and pilot-testing, we mailed the 18-item Likert-type instrument, the Perceived Value of Certification Tool (PVCT), to a sample of 2750 perioperative nurses who had earned the CNOR or CRNFA credential or both. A total of 1398 surveys were returned (50.8% response rate). Factor analysis extracted three factors, accounting for 61% of the variance: personal value, recognition by others, and professional practice. Internal consistency reliability testing (Cronbach\u27s α) identified a standardized α of .924. Over 90% of respondents agreed or strongly agreed with statements about the value of certification related to feelings of personal accomplishment and satisfaction, validating specialized knowledge, indicating professional growth, attainment of a practice standard, personal challenge, and professional commitment, challenge, and credibility. These results are consistent with previously published literature on specialty certification in nursing

The Aquatic Trophic Ecology of Suisun Marsh, San Francisco Estuary, California, During Autumn in a Wet Year

Using stable isotopes of carbon (δ13C) and nitrogen (δ15N) and mixing models, we investigated the trophic levels and carbon sources of invertebrates and fishes of a large tidal marsh in the San Francisco Estuary. Our goal was to better understand an estuarine food web comprised of native and alien species. We found the following: (1) the food web was based largely on carbon from phytoplankton and emergent-aquatic and terrestrial vegetation, but carbon from submerged aquatic vegetation and phytobenthos was also used; (2) alien species increased the complexity of the food web by altering carbon-flow pathways and by occupying trophic positions different from native species; and (3) most consumers were dietary generalists

Executive deficits are related to the inferior frontal junction in early dementia

Executive functions describe a wide variety of higher order cognitive processes that allow the flexible modification of thought and behaviour in response to changing cognitive or environmental contexts. Their impairment is common in neurodegenerative disorders. Executive deficits negatively affect everyday activities and hamper the ability to cope with other deficits, such as memory impairment in Alzheimer's disease or behavioural disorders in frontotemporal lobar degeneration. Our study aimed to characterize the neural correlates of executive functions by relating respective deficits to regional hypometabolism in early dementia. Executive functions were assessed with two classical tests, the Stroop and semantic fluency test and various subtests of the behavioural assessment of the dysexecutive syndrome test battery capturing essential aspects of executive abilities relevant to daily living. Impairments in executive functions were correlated with reductions in brain glucose utilization as measured by [18F]fluorodeoxyglucose positron emission tomography and analysed voxelwise using statistical parametric mapping in 54 subjects with early dementia, mainly Alzheimer's disease and frontotemporal lobar degeneration, and its prodromal stages: subjective and mild cognitive impairment. Although the analysis revealed task-specific frontoparietal networks, it consistently showed that hypometabolism in one region in the left lateral prefrontal cortex—the inferior frontal junction area—was related to performance in the various neuropsychological tests. This brain region has recently been related to the three component processes of cognitive control—working memory, task switching and inhibitory control. Group comparisons additionally showed hypometabolism in this area in Alzheimer's disease and frontotemporal lobar degeneration. Our study underlines the importance of the inferior frontal junction area for cognitive control in general and for executive deficits in early dementia

Between images and built form: Automating the recognition of standardised building components using deep learning

Building on the richness of recent contributions in the field, this paper presents a state-of-the-art CNN analysis method for automatingthe recognition of standardised building components in modern heritage buildings. At the turn of the twentieth century manufacturedbuilding components became widely advertised for specification by architects. Consequently, a form of standardisation across varioustypologies began to take place. During this era of rapid economic and industrialised growth, many forms of public building wereerected. This paper seeks to demonstrate a method for informing the recognition of such elements using deep learning to recognise'families' of elements across a range of buildings in order to retrieve and recognise their technical specifications from the contemporarytrade literature. The method is illustrated through the case of Carnegie Public Libraries in the UK, which provides a unique butubiquitous platform from which to explore the potential for the automated recognition of manufactured standard architecturalcomponents. The aim of enhancing this knowledge base is to use the degree to which these were standardised originally as a means toinform and so support their ongoing care but also that of many other contemporary buildings. Although these libraries are numerous,they are maintained at a local level and as such, their shared challenges for maintenance remain unknown to one another. Additionally,this paper presents a methodology to indirectly retrieve useful indicators and semantics, relating to emerging HBIM families, byapplying deep learning to a varied range of architectural imagery

Methicillin resistant S. aureus (MRSA) in the pork food chain in Germany

This paper gives an overview on studies carried out in Germany on the prevalence of MRSA on different stages of the pork food chain. Prevalence studies were carried out on herd level for breeding (201 herds) and fattening pits (290 herds), at abattoirs (n=1026 pigs), in a pork processing facility and in pig meat at retail. MRSA were characterized using spa-typing SCCmec-typing and testing for antimicrobial resistance

Reduced haemodynamic response in the ageing visual cortex measured by absolute fNIRS

The effect of healthy ageing on visual cortical activation is still to be fully explored. This study aimed to elucidate whether the haemodynamic response (HDR) of the visual cortex altered as a result of ageing. Visually normal (healthy) participants were presented with a simple visual stimulus (reversing checkerboard). Full optometric screening was implemented to identify two age groups: younger adults (n = 12, mean age 21) and older adults (n = 13, mean age 71). Frequency-domain Multi-distance (FD-MD) functional Near-Infrared Spectroscopy (fNIRS) was used to measure absolute changes in oxygenated [HbO] and deoxygenated [HbR] haemoglobin concentrations in the occipital cortices. Utilising a slow event-related design, subjects viewed a full field reversing checkerboard with contrast and check size manipulations (15 and 30 minutes of arc, 50% and 100% contrast). Both groups showed the characteristic response of increased [HbO] and decreased [HbR] during stimulus presentation. However, older adults produced a more varied HDR and often had comparable levels of [HbO] and [HbR] during both stimulus presentation and baseline resting state. Younger adults had significantly greater concentrations of both [HbO] and [HbR] in every investigation regardless of the type of stimulus displayed (p<0.05). The average variance associated with this age-related effect for [HbO] was 88% and [HbR] 91%. Passive viewing of a visual stimulus, without any cognitive input, showed a marked age-related decline in the cortical HDR. Moreover, regardless of stimulus parameters such as check size, the HDR was characterised by age. In concurrence with present neuroimaging literature, we conclude that the visual HDR decreases as healthy ageing proceeds