Knowledge and Perceived Practices on Traumatic Dental Injury (TDI) Management among Primary Caregivers in a Selected Elementary School in Silang, Cavite

Traumatic Dental Injuries (TDI) are unpredictable occurrences and are considered a health problem in a child’s life because of their negative impact. Most TDI incidences happen when a child is at home or school; thus, it is essential that those who are in close contact with them know how to properly manage it. A selected College of Dentistry Clinic at a private sectarian university in Cavite conducted a study to assess primary caregivers' knowledge and perceived practices of primary caregivers on TDI management. The research study included 120 primary caregivers of pupils enrolled in Grades 1-6 in a private Elementary School in Cavite. This quantitative descriptive research design, correlational, and comparative design. A dichotomous scale was used to determine the level of knowledge, and a 4-point Likert scale for the extent of perceived practices. The statistical treatments used included the mean and standard deviation, Mann-Whitney Test U Test, Dwass-Steel-Critchlow-Fligner test, and one-way non-parametric ANOVA (Kruskal-Wallis). Results led researchers to come up with the following conclusions: (1) the level of knowledge of the primary caregivers are high; (2) the extent of the perceived practices of the primary caregivers are good; (3) knowledge and perceived practices on TDI management are significantly correlated, which means that the practices of the primary caregivers are dependent on the prior knowledge they acquired on TDI management; (4) a significant difference was found in the knowledge of primary caregivers considering their sex; (5) a significant difference was found in the knowledge of primary caregivers that have a Masters or Doctoral Degree. Keywords: Traumatic dental injury, TDI management, primary caregivers, avulsion, reimplantatio

Strategies for meiotic sex chromosome dynamics and telomeric elongation in Marsupials

During meiotic prophase I, homologous chromosomes pair, synapse and recombine in a tightly regulated process that ensures the generation of genetically variable haploid gametes. Although the mechanisms underlying meiotic cell division have been well studied in model species, our understanding of the dynamics of meiotic prophase I in non-traditional model mammals remains in its infancy. Here, we reveal key meiotic features in previously uncharacterised marsupial species (the tammar wallaby and the fat-tailed dunnart), plus the fat-tailed mouse opossum, with a focus on sex chromosome pairing strategies, recombina tion and meiotic telomere homeostasis. We uncovered differences between phylogroups with important functional and evolutionary implications. First, sex chromosomes, which lack a pseudo-autosomal region in marsupials, had species specific pairing and silencing strategies, with implications for sex chromosome evolution. Second, we detected two waves of γH2AX accumulation during prophase I. The first wave was accompanied by low γH2AX lev els on autosomes, which correlated with the low recombination rates that distinguish marsupials from eutherian mammals. In the second wave, γH2AX was restricted to sex chromosomes in all three species, which correlated with transcription from the X in tammar wallaby. This suggests non-canonical functions of γH2AX on meiotic sex chromosomes. Finally, we uncover evidence for telomere elongation in primary spermatocytes of the fattailed dunnart, a unique strategy within mammals. Our results provide new insights into meiotic progression and telomere homeostasis in marsupials, highlighting the importance of capturing the diversity of meiotic strategies within mammalsThis work was supported by the Ministry of Economy, Industry and Competitiveness (CGL2017-83802-P to A.R-H. and CGL2014- 53106-P to J.P.), the Spanish Ministry of Science and Innovation (PID2020-112557GB-I00 to A.R H.) and the Agència de Gestio´ d’Ajuts Universitaris i de Recerca, AGAUR (SGR1215 to A.R-H.

Multivariate analysis and extraction of parameters in resistive RAMs using the Quantum Point Contact model

A multivariate analysis of the parameters that characterize the reset process in RRAMs has been performed. The different correlations obtained can help to shed light on the current components that contribute in the Low Resistance State (LRS) of the technology considered. In addition, a screening method for the Quantum Point Contact (QPC) current component is presented. For this purpose the second derivative of the current has been obtained using a novel numerical method which allows determining the QPC model parameters. Once the procedure is completed, a whole RS series of thousands of curves is studied by means of a genetic algorithm. The extracted QPC parameter distributions are characterized in depth to get information about the filamentary pathways associated with LRS in the low voltage conduction regime.Spanish Ministry of Economy and Competitiveness TEC2014-52152-C3-2-R , MTM2013-47929-P (also supported by the FEDER program)IMB-CNM Spanish Ministry of Economy and Competitiveness TEC2014-52152-C3-1-R and TEC2014-54906-JIN (supported by the FEDER program)ENIAC Joint Undertaking-PANACHE project.Spanish ICTS Network MICRONANOFAB

Effect of competition & thin models on body dissatisfaction

This is a study conducted with the aim of learning more about the effects of competition in TV shows to teenage Female De La Salle University-Manila students with regards to their body dissatisfaction and the role of thin models in the process of feeling dissatisfied with their bodies. The researchers conducted an experiment where 60 participants were placed in 1 of 4 possible conditions randomly and were asked answer a questionnaire then to watch a video clip. Then, the participants were asked to answer a survey. The results showed that competition in the models had no effect on the body dissatisfaction of females

TGGE analysis of the diversity of ammonia-oxidizing and denitrifying bacteria in submerged filter biofilms for the treatment of urban wastewater

The spatial and temporal diversity of the bacterial community-forming biofilms in a pilot-scale submerged biofilter used for the treatment of urban wastewater was analyzed by a temperature-gradient gel electrophoresis (TGGE) approach. TGGE profiles based on partial sequence of the 16S rRNA gene showed that the community composition of the biofilms remained fairly stable along the column system and during the whole time of operation of the biofilter (more than 1 year). Community-profiling based on the amplification and separation of partial ammonia monooxygenase (amoA) and nitrous oxide reductase (nosZ) genes demonstrated that ammonia-oxidizing and denitrifying bacteria coexisted in both the anoxic and the aerated parts of the system. Several amoA and nosZ bands separated by TGGE were reamplified and sequenced, in order to further analyze the composition of these microbial communities in the biofilm. Phylogeny inferred from amoA/AmoA revealed the prevalence of Nitrosomonas species with five sequences affiliated to Nitrosomonas oligotropha, six sequences affiliated to Nitrosomonas europaea, and three sequences that showed only 75.7-76.1% identity of the DNA sequence with the closest described species (Nitrosomonas nitrosa). According to literature, this low identity value is indicative of previously undiscovered species. Eighteen new partial nosZ sequences were obtained which were mostly related to nosZ of gamma-proteobacteria (Pseudomonas) or clustered in the periphery of previously known denitrifying alpha-proteobacteria (Bradyrhizobium and Azospirillum)

Dominance of sphingomonads in a copper-exposed biofilm community for groundwater treatment

The structure, biological activity and microbial biodiversity of a biofilm used for the removal of copper from groundwater were studied and compared with those of a biofilm grown under copper-free conditions. A laboratory-scale submerged fixed biofilter was fed with groundwater (2.3 l h(-1)) artificially polluted with Cu(II) (15 mg l(-1)) and amended with sucrose (150 mg l(-1)) as carbon source. Between 73 and 90% of the Cu(II) was removed from water during long-term operation (over 200 days). The biofilm was a complex ecosystem, consisting of eukaryotic and prokaryotic micro-organisms. Scanning electron microscopy revealed marked structural changes in the biofilm induced by Cu(II), compared to the biofilm grown in absence of the heavy metal. Analysis of cell-bound extracellular polymeric substances (EPS) demonstrated a significant modification of the composition of cell envelopes in response to Cu(II). Transmission electron microscopy and energy-dispersive X-ray microanalysis (EDX) showed that copper bioaccumulated in the EPS matrix by becoming bound to phosphates and/or silicates, whereas copper accumulated only intracytoplasmically in cells of eukaryotic microbes. Cu(II) also decreased sucrose consumption, ATP content and alkaline phosphatase activity of the biofilm. A detailed study of the bacterial community composition was conducted by 16S rRNA-based temperature gradient gel electrophoresis (TGGE) profiling, which showed spatial and temporal stability of the species diversity of copper-exposed biofilms during biofilter operation. PCR reamplification and sequencing of 14 TGGE bands showed the prevalence of alphaproteobacteria, with most sequences (78%) affiliated to the Sphingomonadaceae. The major cultivable colony type in plate counts of the copper-exposed biofilm was also identified as that of Sphingomonas sp. These data confirm a major role of these organisms in the composition of the Cu(II)-removing community