Characteristics and Needs of Children and Adolescents Diagnosed with Type 2 Diabetes Mellitus in Santa Clara County, California

Objective: The study objectives were to examine the characteristics and perceived needs of children and adolescents diagnosed with type 2 diabetes. Design: Data were gathered via self-administered questionnaires pertaining to demographics, family history of type 2 diabetes, physical characteristics, lifestyle/exercise behaviors, dietary behaviors and interests in meeting other youth with type 2 diabetes. Subjects and their parents/guardians were asked to complete separate questionnaires and afterwards, each youth participated in a follow-up interview related to diabetes management. Subjects: Participants (N=14) were obtained from a population of youth diagnosed with type 2 diabetes seeking medical care at the Kaiser Santa Teresa Pediatric Endocrine Clinic in Santa Clara, CA. Eleven of the subjects were Latino (79%) and the remaining 3 were Asian American (21%). Subjects ranged in age from 10 to 18 years with a mean age of 13.8 ± 2.3 years. The mean age at diagnosis was 12.7 ± 2.3 years with a range from eightand-a-half years to 17 years when first diagnosed. The mean body mass index (BMI) of the participants was 33.5 ± 6.2 kg/m2 . Statistical Analysis: Descriptive statistics were utilized for all analyses including discrete and continuous data to determine counts, percentages, means, and standard deviations. Results: The youth characteristics in the present study were consistent with previous studies that have examined the demographics, physical characteristics, and family history of type 2 diabetes among youth in the United States. Acanthosis nigricans, a hyperinsulinemic condition, was present in 10 of the 14 (71%) of the youth and most were overweight or obese with a mean BMI of 33.5 ± 6.2 kg/m2 . A positive family history for diabetes was evident in most subjects, however, this positive history did not lead to an increase in diabetes knowledge among youth. Most youth subjects were sedentary and were more likely to make dietary changes than exercise changes to manage their diabetes. Interests in attending youth summer camps or family camps geared towards diabetes education were quite high. As the characteristics and diabetes risk factors of type 2 diabetes among youth continue to be identified, and as it continues to increase in this population, culturally appropriate prevention programs will need to be designed and implemented to address the needs of this unique population

Feeding a Novel Trace Mineral at Lower Levels to Grazing Stocker Cattle Does Not Impair Performance

When grazing stocker cattle on native Flint Hills pasture, optimizing growth rate is important in determining overall profitability. The correct selection of mineral supplements is an important decision that can be used to help promote overall productivity during a grazing season

Evaluation of Trace Mineral Sources on Newly Arrived Stocker Cattle

Light weight stocker calves experience variable degrees of physiological and psychological stressors as they are assembled from various marketing points and transported to their destination. Susceptibility to disease in young, long hauled calves is greatly enhanced and the consequence of sickness is a major cause for poor production outcomes. Enhanced nutritional trace mineral programs that contain zinc, copper, and manganese from organic or hydroxy sources may be more efficacious as a means of minimizing disease or realizing improved performance than the sulfate form of these respective trace minerals

These aren’t the loci you’re looking for: Principles of effective SNP filtering for molecular ecologists

Sequencing reduced‐representation libraries of restriction site‐associated DNA (RADseq) to identify single nucleotide polymorphisms (SNPs) is quickly becoming a standard methodology for molecular ecologists. Because of the scale of RADseq data sets, putative loci cannot be assessed individually, making the process of filtering noise and correctly identifying biologically meaningful signal more difficult. Artefacts introduced during library preparation and/or bioinformatic processing of SNP data can create patterns that are incorrectly interpreted as indicative of population structure or natural selection. Therefore, it is crucial to carefully consider types of errors that may be introduced during laboratory work and data processing, and how to minimize, detect and remove these errors. Here, we discuss issues inherent to RADseq methodologies that can result in artefacts during library preparation and locus reconstruction resulting in erroneous SNP calls and, ultimately, genotyping error. Further, we describe steps that can be implemented to create a rigorously filtered data set consisting of markers accurately representing independent loci and compare the effect of different combinations of filters on four RAD data sets. At last, we stress the importance of publishing raw sequence data along with final filtered data sets in addition to detailed documentation of filtering steps and quality control measures

Evaluation of the Productivity of a Single Subcutaneous Injection of LongRange in Stocker Calves Compared With a Positive (Dectomax) and a Negative (Saline) Control

Subclinical parasitism is commonly observed in stocker cattle. Treatment of internal parasites helps to improve weight gains, feed conversion, and immune status and decreases morbidity and mortality of beef cattle (Hawkins, 1993). Some of the most concerning classes of internal parasites include Cooperia, Haemonchus, and Ostertagia. Commonly used anthelmintics come in the form of pour-ons, oral drenches, and subcutaneous injections. A majority of these drugs are designed to be administered in a single dose and provide defense against stomach worms for approximately 14 to 42 days, but the typical grazing season lasts for approximately 120 days. For grazing cattle to have season-long protection from parasites, they may require a second dose of anthelmintic treatment, which would require cattle to be gathered and processed through a chute in the middle of the grazing season. LongRange (Merial, Duluth, GA) is the first single-dose extended release anthelmintic that provides approximately 100 to 150 days of protection. This is accomplished by combining two forms of the active ingredient: one that is released into the blood immediately after injection and a second that consists of a slow-release polymer that releases the active ingredient gradually throughout the grazing period. The objective of this study was to measure body weight productivity, fecal egg counts, and fly repellent capabilities of LongRange when administered once subcutaneously at 1.0 mg/kg body weight as a long-acting solution compared with a commercially available injectable (Dectomax; Zoetis, Florham Park, NJ) and saline in stocker cattle

Evaluation of Calcium Hydroxide-Treated Stover (Second Crop) in Receiving and Growing Diets and Effects on Cattle Performance

Production of ethanol from corn as an alternative fuel source has significantly affected the pricing landscape for corn as an energy source for livestock. As ethanol usage has become more prevalent, corn prices have become more volatile, especially in critical corn-growing areas that have been affected by drought. Consequently, many beef cattle feeders have become interested in alternative energy sources in an effort to control cost of gain. Second Crop (ADM Corp., Decatur, IL) is a process in which calcium hydroxide is added to fibrous crop residues, such as wheat straw and corn stover. When applied to low-quality roughages, calcium hydroxide disrupts the chemical bonds between lignin and hemicellulose, thus improving digestibility of the fiber by ruminal microbes. Treatment of low-quality forage with the Second Crop process could improve the energy value of forages, effectively decreasing reliance on cereal grains as sources of supplemental energy

2014 dDocent: a RADseq, variantcalling pipeline designed for population genomics of non-model organisms

ABSTRACT 14 Restriction-site associated DNA sequencing (RADseq) has become a powerful and useful 15 approach for population genomics. Currently, no software exists that utilizes both paired-end 16 reads from RADseq data to efficiently produce population-informative variant calls, 17 especially for organisms with large effective population sizes and high levels of genetic 18 polymorphism but for which no genomic resources exist. dDocent is an analysis pipeline with 19 a user-friendly, command-line interface designed to process individually barcoded RADseq 20 data (with double cut sites) into informative SNPs/INDELs for population-level analyses. The 21 pipeline, written in BASH, uses data reduction techniques and other stand-alone software 22 packages to perform quality trimming and adapter removal, de novo assembly of RAD loci, 23 read mapping, SNP and INDEL calling, and baseline data filtering. Double-digest RAD data 24 from population pairings of three different marine fishes were used to compare dDocent with 25 Stacks, the first generally available, widely used pipeline for analysis of RADseq data. 26 dDocent consistently identified more SNPs shared across greater numbers of individuals and 27 with higher levels of coverage. This is most likely due to the fact that dDocent quality trims 28 instead of filtering and incorporates both forward and reverse reads in assembly, mapping, 29 and SNP calling, thus enabling use of reads with INDEL polymorphisms. The pipeline and a 30 comprehensive user guide can be found at (http://dDocent.wordpress.com). 31 32 PeerJ PrePrints | http://dx.doi.org/10.7287/peerj.preprints.314v1 | CC-BY 4.

Recommendations for Child Care Centers

Design guide for planning, programming and designing different types of child care facilities. Includes 115 patterns for the policy, planning and design of large centers, neighborhood centers and family day-care homes. Based on current research information. Highly illustrated with photographs and sketches. Received an Award for Applied Research from Progressive Architecture, 1980, and received the 1980 UWM Foundation Research Award. Reprinted 1981, with new photographs in 1984, 1988 and in 1991. Revised edition 1994.https://dc.uwm.edu/caupr_mono/1032/thumbnail.jp