494 research outputs found
The Stellar Populations of the Carina Dwarf Spheroidal Galaxy: I. a New Color-Magnitude Diagram for the Giant and Horizontal Branches
We report on the first in a series of studies of the Carina dwarf spheroidal
galaxy, a nearby satellite of our Galaxy. Our two major results are: 1) precise
BI photometry (\sigma_{B-I} \simlt 0.05 for V \simlt 22) for 11,489 stars
in the Carina field, and 2) the detection of two, morphologically distinct,
horizontal branches, which confirms that star formation in Carina occurred in
two well-separated episodes. The old horizontal branch and RR Lyrae instability
strip belong to a > 10 Gyr stellar population, while the populous red-clump
horizontal branch belongs to an approximately 6 Gyr stellar population. We
derive a distance modulus for Carina from the apparent
magnitudes of the old horizontal branch and the tip of the red giant branch,
and discuss modifications to the previously estimated distance, total
magnitude, and stellar ages. Using the color of the red giant branch, we
estimate the metallicities of the younger and older populations to be [Fe/H] =
-2.0 and -2.2, respectively.Comment: 18 pages, 9 figures, uses AAS LaTex macros, PostScript figures
available through anonymous ftp, accepted for publication in the Astronomical
Journal, DAO-tsh94-
The genome sequence of the biocontrol fungus Metarhizium anisopliae and comparative genomics of Metarhizium species.
Background: Metarhizium anisopliae is an important fungal biocontrol agent of insect pests of agricultural crops. Genomics can aid the successful commercialization of biopesticides by identification of key genes differentiating closely related species, selection of virulent microbial isolates which are amenable to industrial scale production and formulation and through the reduction of phenotypic variability. The genome of Metarhizium isolate ARSEF23 was recently published as a model for M. anisopliae, however phylogenetic analysis has since re-classified this isolate as M. robertsii. We present a new annotated genome sequence of M. anisopliae (isolate Ma69) and whole genome comparison to M. robertsii (ARSEF23) and M. acridum (CQMa 102).Results: Whole genome analysis of M. anisopliae indicates significant macrosynteny with M. robertsii but with some large genomic inversions. In comparison to M. acridum, the genome of M. anisopliae shares lower sequence homology. While alignments overall are co-linear, the genome of M. acridum is not contiguous enough to conclusively observe macrosynteny. Mating type gene analysis revealed both MAT1-1 and MAT1-2 genes present in M. anisopliae suggesting putative homothallism, despite having no known teleomorph, in contrast with the putatively heterothallic M. acridum isolate CQMa 102 (MAT1-2) and M. robertsii isolate ARSEF23 (altered MAT1-1). Repetitive DNA and RIP analysis revealed M. acridum to have twice the repetitive content of the other two species and M. anisopliae to be five times more RIP affected than M. robertsii. We also present an initial bioinformatic survey of candidate pathogenicity genes in M. anisopliae.Conclusions: The annotated genome of M. anisopliae is an important resource for the identification of virulence genes specific to M. anisopliae and development of species- and strain- specific assays. New insight into the possibility of homothallism and RIP affectedness has important implications for the development of M. anisopliae as a biopesticide as it may indicate the potential for greater inherent diversity in this species than the other species. This could present opportunities to select isolates with unique combinations of pathogenicity factors, or it may point to instability in the species, a negative attribute in a biopesticide
A novel mode of chromosomal evolution peculiar to filamentous Ascomycete fungi
Background: Gene loss, inversions, translocations, and other chromosomal rearrangements vary among species, resulting in different rates of structural genome evolution. Major chromosomal rearrangements are rare in most eukaryotes, giving large regions with the same genes in the same order and orientation across species. These regions of macrosynteny have been very useful for locating homologous genes in different species and to guide the assembly of genome sequences. Previous analyses in the fungi have indicated that macrosynteny is rare; instead, comparisons across species show no synteny or only microsyntenic regions encompassing usually five or fewer genes. To test the hypothesis that chromosomal evolution is different in the fungi compared to other eukaryotes, synteny was compared between species of the major fungal taxa. Results: These analyses identified a novel form of evolution in which genes are conserved within homologous chromosomes, but with randomized orders and orientations. This mode of evolution is designated mesosynteny, to differentiate it from micro-and macrosynteny seen in other organisms. Mesosynteny is an alternative evolutionary pathway very different from macrosyntenic conservation. Surprisingly, mesosynteny was not found in all fungal groups. Instead, mesosynteny appears to be restricted to filamentous Ascomycetes and was most striking between species in the Dothideomycetes. Conclusions: The existence of mesosynteny between relatively distantly related Ascomycetes could be explained by a high frequency of chromosomal inversions, but translocations must be extremely rare. The mechanism for this phenomenon is not known, but presumably involves generation of frequent inversions during meiosis
The Star Formation History of the Large Magellanic Cloud
Using WFPC2 aboard the Hubble Space Telescope, we have created deep
color-magnitude diagrams in the V and I passbands for approximately 100,000
stars in a field at the center of the LMC bar and another in the disk. The
main--sequence luminosity functions (LFs) from 19 mag < V < 23.5 mag, the red
clump and horizontal branch morphologies, and the differential Hess diagram of
the two fields all strongly imply that the disk and bar have significantly
different star-formation histories (SFHs). The disk's SFH has been relatively
smooth and continuous over the last 15 Gyr while the bar's SFH was dominated by
star formation episodes at intermediate ages. Comparison of the LF against
predictions based on Padova theoretical stellar evolution models and an assumed
age-metallicity relationship allows us to identify the dominant stellar
populations in the bar with episodes of star formation that occurred from 4 to
6 and 1 to 2 Gyr ago. These events accounted, respectively, for approximately
25% and 15% of its stellar mass. The disk field may share a mild enhancement in
SF for the younger episode, and thus we identify the 4 to 6 Gyr episode with
the formation of the LMC bar.Comment: 14 pages, 5 figures, Latex, also available at
http://www.ps.uci.edu/physics/smeckerhane.html. Accepted for publication in
Ap
Forced and self-excited oscillations of an optomechanical cavity
We experimentally study forced and self oscillations of an optomechanical
cavity which is formed between a fiber Bragg grating that serves as a static
mirror and between a freely suspended metallic mechanical resonator that serves
as a moving mirror. In the domain of small amplitude mechanical oscillations,
we find that the optomechanical coupling is manifested as changes in the
effective resonance frequency, damping rate and cubic nonlinearity of the
mechanical resonator. Moreover, self oscillations of the micromechanical mirror
are observed above a certain optical power threshold. A comparison between the
experimental results and a theoretical model that we have recently presented
yields a good agreement. The comparison also indicates that the dominant
optomechanical coupling mechanism is the heating of the metallic mirror due to
optical absorption.Comment: 11 pages, 6 figure
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