Maximizing System Throughput Using Cooperative Sensing in Multi-Channel Cognitive Radio Networks

In Cognitive Radio Networks (CRNs), unlicensed users are allowed to access the licensed spectrum when it is not currently being used by primary users (PUs). In this paper, we study the throughput maximization problem for a multi-channel CRN where each SU can only sense a limited number of channels. We show that this problem is strongly NP-hard, and propose an approximation algorithm with a factor at least $1/2\mu$ where $\mu \in [1,2]$ is a system parameter reflecting the sensing capability of SUs across channels and their sensing budgets. This performance guarantee is achieved by exploiting a nice structural property of the objective function and constructing a particular matching. Our numerical results demonstrate the advantage of our algorithm compared with both a random and a greedy sensing assignment algorithm

A Methodology for daylight optimisation of high-rise buildings in the dense urban district using overhang length and glazing type variables with surrogate modelling

Urbanization and population growth lead to the construction of higher buildings in the 21st century. This causes an increment on energy consumption as the amount of constructed floor areas is rising steadily. Integrating daylight performance in building design supports reducing the energy consumption and satisfying occupants' comfort. This study presents a methodology to optimise the daylight performance of a high-rise building located in a dense urban district. The purpose is to deal with optimisation problems by dividing the high-rise building into five zones from the ground level to the sky level, to achieve better daylight performance. Therefore, the study covers five optimization problems. Overhang length and glazing type are considered to optimise spatial Daylight Autonomy (sDA) and Annual Sunlight Exposure (ASE). A total of 500 samples in each zone are collected to develop surrogate models. A self-adaptive differential evolution algorithm is used to obtain near-optimal results for each zone. The developed surrogate models can estimate the metrics with minimum 98.25% R2 which is calculated from neural network prediction and Diva simulations. In the case study, the proposed methodology improves daylight performance of the high-rise building, decreasing ASE by approx. 27.6% and increasing the sDA values by around 88.2% in the dense urban district. - Published under licence by IOP Publishing Ltd.We would like to thank Cemre Cubukcuoglu for the collaborative work while implementing the optimisation algorithm. M. Fatih Tasgetiren, who is partially supported by the National Natural Science Foundation of China (Grant No. 51435009), acknowledges the HUST project in Wuhan.Scopu

GPFrontend and GPGraphics: graphical analysis tools for genetic association studies

<p>Abstract</p> <p>Background</p> <p>Most software packages for whole genome association studies are non-graphical, purely text based programs originally designed to run with UNIX-like operating systems. Graphical output is often not intended or supposed to be performed with other command line tools, e.g. gnuplot.</p> <p>Results</p> <p>Using the Microsoft .NET 2.0 platform and Visual Studio 2005, we have created a graphical software package to analyze data from microarray whole genome association studies, both for a DNA-pooling based approach as well as regular single sample data. Part of this package was made to integrate with GenePool 0.8.2, a previously existing software suite for GNU/Linux systems, which we have modified to run in a Microsoft Windows environment. Further modifications cause it to generate some additional data. This enables GenePool to interact with the .NET parts created by us. The programs we developed are GPFrontend, a graphical user interface and frontend to use GenePool and create metadata files for it, and GPGraphics, a program to further analyze and graphically evaluate output of different WGA analysis programs, among them also GenePool.</p> <p>Conclusions</p> <p>Our programs enable regular MS Windows users without much experience in bioinformatics to easily visualize whole genome data from a variety of sources.</p

Consumer acculturation as a dialogical process: Case studies from rural-to-urban migrants in Turkey

[No abstract available

CRISPR/Cas9-Mediated Knock-Out of KrasG12D Mutated Pancreatic Cancer Cell Lines

In 90% of pancreatic ductal adenocarcinoma cases, genetic alteration of the proto-oncogene Kras has occurred, leading to uncontrolled proliferation of cancerous cells. Targeting Kras has proven to be difficult and the battle against pancreatic cancer is ongoing. A promising approach to combat cancer was the discovery of the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system, which can be used to genetically modify cells. To assess the potential of a CRISPR/CRISPR-associated protein 9 (Cas9) method to eliminate Kras mutations in cells, we aimed to knock-out the c.35G>A (p.G12D) Kras mutation. Therefore, three cell lines with a heterozygous Kras mutation (the human cell lines SUIT-2 and Panc-1 and the cell line TB32047 from a KPC mouse model) were used. After transfection, puromycin selection and single-cell cloning, proteins from two negative controls and five to seven clones were isolated to verify the knock-out and to analyze changes in key signal transduction proteins. Western blots showed a specific knock-out in the KrasG12D protein, but wildtype Kras was expressed by all of the cells. Signal transduction analysis (for Erk, Akt, Stat3, AMPKα, and c-myc) revealed expression levels similar to the wildtype. The results described herein indicate that knocking-out the KrasG12D mutation by CRISPR/Cas9 is possible. Additionally, under regular growth conditions, the knock-out clones resembled wildtype cells

EPIDEMIOLOGICAL CHARACTERISTICS OF VIRAL HEPATITIS IN PATIENTS WITH RHEUMATIC DISEASES - IMPLICATIONS FROM TREASURE DATABASE

EULAR European Congress of Rheumatology (EULAR) -- JUN 01-04, 2022 -- Copenhagen, DENMARK[Abstract Not Available]European Alliance Assoc Rheumato

Tracing Myelin Protein Zero (P0) in vivo by construction of P0-GFP fusion proteins

BACKGROUND: Mutations in P0, the major protein of the myelin sheath in peripheral nerves, cause the inherited peripheral neuropathies Charcot-Marie-Tooth disease type 1B (CMT1B), Dejerine-Sottas syndrome (DSS) and congenital hypomyelination (CH). We reported earlier a de novo insertional mutation c.662_663GC (Ala221fs) in a DSS patient. The c.662_663GC insertion results in a frame shift mutation Ala221fs altering the C-terminal amino acid sequence. The adhesion-relevant intracellular RSTK domain is replaced by a sequence similar to Na+/K+ ATPase. To further clarify the molecular disease mechanisms in this sporadic patient we constructed wild type P0 and the c.662_663GC mutant expression cassettes by site-specific mutagenesis and transfected the constructs into insect cells (S2, High5). To trace the effects in live cells, green fluorescent protein (GFP) has been added to the carboxyterminus of the wild type and mutated P0 protein. RESULTS: In contrast to the membrane-localized wild type P0-GFP the Ala221fs P0-GFP protein was detectable almost only in the cytoplasm of the cells, and a complete loss of adhesion function was observed. CONCLUSIONS: The present study provides evidence that GFP is a versatile tool to trace in vivo effects of P0 and its mutations. Not only a loss of adhesion function as a result of the loss of the RSTK domain, but also altered intracellular trafficking indicated by a loss of membrane insertion are possible consequences of the Ala221fs mutation

Molecular differentiation between osteophytic and articular cartilage – clues for a transient and permanent chondrocyte phenotype

SummaryObjectiveTo identify the molecular differences between the transient and permanent chondrocyte phenotype in osteophytic and articular cartilage.MethodsTotal RNA was isolated from the cartilaginous layer of osteophytes and from intact articular cartilage from knee joints of 15 adult human donors and subjected to cDNA microarray analysis. The differential expression of relevant genes between these two cartilaginous tissues was additionally validated by quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and by immunohistochemistry.ResultsAmong 47,000 screened transcripts, 600 transcripts were differentially expressed between osteophytic and articular chondrocytes. Osteophytic chondrocytes were characterized by increased expression of genes involved in the endochondral ossification process [bone gamma-carboxyglutamate protein/osteocalcin (BGLAP), bone morphogenetic protein-8B (BMP8B), collagen type I, alpha 2 (COL1A2), sclerostin (SOST), growth arrest and DNA damage-induced gene 45ß (GADD45ß), runt-related transcription factor 2 (RUNX2)], and genes encoding tissue remodeling enzymes [matrix metallopeptidase (MMP)9, 13, hyaluronan synthase 1 (HAS1)]. Articular chondrocytes expressed increased transcript levels of antagonists and inhibitors of the BMP- and Wnt-signaling pathways [Gremlin-1 (GREM1), frizzled-related protein (FRZB), WNT1 inducible signaling pathway protein-3 (WISP3)], as well as factors that inhibit terminal chondrocyte differentiation and endochondral bone formation [parathyroid hormone-like hormone (PTHLH), sex-determining region Y-box 9 (SOX9), stanniocalcin-2 (STC2), S100 calcium binding protein A1 (S100A1), S100 calcium binding protein B (S100B)].Immunohistochemistry of tissue sections for GREM1 and BGLAP, the two most prominent differentially expressed genes, confirmed selective detection of GREM1 in articular chondrocytes and that of BGLAP in osteophytic chondrocytes and bone.ConclusionsOsteophytic and articular chondrocytes significantly differ in their gene expression pattern. In articular cartilage, a prominent expression of antagonists inhibiting the BMP- and Wnt-pathway may serve to lock and stabilize the permanent chondrocyte phenotype and thus prevent their terminal differentiation. In contrast, osteophytic chondrocytes express genes with roles in the endochondral ossification process, which may account for their transient phenotype

On Motion of Robot End-Effector Using the Curvature Theory of Timelike Ruled Surfaces with Timelike Rulings

The trajectory of a robot end-effector is described by a ruled surface and a spin angle about the ruling of the ruled surface. In this way, the differential properties of motion of the end-effector are obtained from the well-known curvature theory of a ruled surface. The curvature theory of a ruled surface generated by a line fixed in the end-effector referred to as the tool line is used for more accurate motion of a robot end-effector. In the present paper, we first defined tool trihedron in which tool line is contained for timelike ruled surface with timelike ruling, and transition relations among surface trihedron: tool trihedron, generator trihedron, natural trihedron, and Darboux vectors for each trihedron, were found. Then differential properties of robot end-effector&apos;s motion were obtained by using the curvature theory of timelike ruled surfaces with timelike ruling