9 research outputs found

    Molecular Effects of Doxycycline Treatment on Pterygium as Revealed by Massive Transcriptome Sequencing

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    Pterygium is a lesion of the eye surface which involves cell proliferation, migration, angiogenesis, fibrosis, and extracellular matrix remodelling. Surgery is the only approved method to treat this disorder, but high recurrence rates are common. Recently, it has been shown in a mouse model that treatment with doxycycline resulted in reduction of the pterygium lesions. Here we study the mechanism(s) of action by which doxycycline achieves these results, using massive sequencing techniques. Surgically removed pterygia from 10 consecutive patients were set in short term culture and exposed to 0 (control), 50, 200, and 500 µg/ml doxycycline for 24 h, their mRNA was purified, reverse transcribed and sequenced through Illumina’s massive sequencing protocols. Acquired data were subjected to quantile normalization and analyzed using cytoscape plugin software to explore the pathways involved. False discovery rate (FDR) methods were used to identify 332 genes which modified their expression in a dose-dependent manner upon exposure to doxycycline. The more represented cellular pathways included all mitochondrial genes, the endoplasmic reticulum stress response, integrins and extracellular matrix components, and growth factors. A high correlation was obtained when comparing ultrasequencing data with qRT-PCR and ELISA results

    Nano identification and tribo testing of explosive welding copper/brass

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    Bimetallic materials are construction materials that are increasingly used in many industries: in the graphic industry, tobacco processing industry for various cutting knives, in the chemical industry for various plants and vessels, food industry, military industry, etc. In the production of ships, their use becomes dominant, primarily as a combination of cheap construction materials highly resistant to corrosion, at a price closer to construction materials. The appearance of plating with the help of explosion energy has significantly increased the range and quality of available multilayer metals. Although stainless steels and aluminum are the most commonly used material for bimetallic and clad materials, materials such as titanium, zirconium or tantalum are increasingly used. The paper analyzes the process of explosion welding and the results of exploration of the explosion of a welded joint of copper and brass. The microhardness of the material in the welded joint, tribological characteristics and scratch test joint were analyzed

    Investigation into the Effects of Roller Pressing on Hardness, Roughness and Elastic Modulus of Wooden Workpieces

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    The paper investigates the effects of roller pressing on the hardness, roughness, and elastic modulus of wooden workpieces. For research purposes, a roller pressing device based on purely mechanical processing was designed and developed. Four different levels of pressing force have been applied to eight different types of wood: cherry, fir, alder, linden, beech, walnut, oak, and ash. The obtained results indicate that the proposed processing method can significantly improve the hardness, elastic modulus, and surface quality of wooden workpieces. The ash sample exhibited the largest relative increase in hardness (175.9%), while the most significant relative increase in the elastic modulus (66.73%) was measured on the linden sample. The largest relative decrease in surface roughness (54.75%) was achieved on the alder sample. For all types of wood except for fir, in which case an increase in pressing force did not produce the desired reduction of roughness, correlation coefficients indicate a strong relationship between the pressing force as an input variable and the elastic modulus, hardness, and roughness as output variables

    INFLUENCE OF VANADIUM CONTENT ON THE TRIBOLOGICAL BEHAVIOUR OF X140CrMol2-l AIR-HARDENING STEEL

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    The paper presents experimental testing of wear resistance of steel samples from the same groups of steel. Test results were recorded and presented in the form of diagrams showing the wear resistance of the tested materials in different sliding conditions. The tested steels have high carbon content; the addition of chromium and molybdenum results in the high hardness and low impact toughness of the steels. The addition of vanadium changes the microstructure when the metal grain becomes smaller and the whole structure is martensitic, with chromium and vanadium carbides in the metal matrix. A change in the microstructure causes a change in mechanical properties. The obtained results showed that the addition of vanadium increases impact toughness. However, it is not known how it affects wear resistance and hardness. This type of steel belongs to a new group of steels resistant to wear. A change in the vanadium content causes a decrease in the hardness and wear resistance of the steel and an increase in its impact toughness
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