The use of parasites in discriminating stocks of Pacific halibut (Hippoglossus stenolepis) in the northeast Pacific

The use of parasites as indicators of the stock structure of Pacific halibut (Hippoglossus stenolepis) in the northeast Pacific was investigated by using 328 adult (>55 cm fork length) halibut from 15 composite localities ranging from northern California to the northern Bering Sea and 96 juvenile (10–55 cm) halibut from five localities ranging from the northern Queen Charlotte Islands to the Bering Sea. Counts of eight selected parasite species (the juvenile acanthocephalans Corynosoma strumosum and C. villosum, the metacestode Nybelinia surmenicola, the digenean metacercaria Otodistomum sp., and the larval nematodes Anisakis simplex, Pseudoterranova decipiens, Contracaecum sp., and Spirurid gen. sp.) that produce infections of long duration, do not multiply in the host, and that have a relatively high abundance in at least one geographic locality were subjected to discriminant function analysis. Juvenile Pacific halibut showed no separation and, even though they were not heavily infected with parasites, the analysis suggested that juveniles could be a mixed stock. Three groups of adults were identified: fish from California to the southern Queen Charlotte Islands, those from the northern Queen Charlotte Islands to the central Bering Sea, and those from the central and north-ern Bering Sea. These groups suggest that the single stock concept be more thoroughly evaluated

Calibration of a compact survey probe for pitot pressure, Mach number, and flow angularity measurements

Calibration of compact survey probe for pitot pressure, Mach number, and flow angularity measurement

Chromosomal aberrations in a natural population of chironomus tentans exposed to chronic low-level environmental radiation

The salivary gland chromosomes of Chironomus tentans larvae collected from White Oak Creek, an area contaminated by radioactive waste from the Oak Ridge National Laboratory, and from six uncontaminated areas were examined for chromosomal aberrations. White Oak Creek populations were exposed to absorbed doses as high as 230 rads per year or about 1000 times background. Chromosomal maps were constructed to make a general comparison of the banding pattern of the salivary chromosomes of the C. tentans in the East Tennessee area with those of Canada and Europe. These maps were used as a reference in scoring aberrations. Fifteen different chromosomal aberrations were found in 365 larvae taken from the irradiated population as compared with five different aberrations observed in 356 larvae from six control populations, but the mean number of aberrations per larva did not differ in any of the populations. The quantitative amount of heterozygosity was essentially the same in the irradiated and the control population, but there were three times the variety of chromosomal aberrations found in the irradiated area. From this evidence it was concluded that chronic low-level irradiation from radioactive waste was increasing the variability of chromosomal aberrations without significantly increasing the frequency. It was also concluded that chromosomal polymorphism can be maintained in a natural population without superiority of the heterozygous individuals. (C.H.

Dcse Estimation and Prediction of Radiation Effects on Aquatic Biota Resulting from Radioactive Releases .from the Nuclear Fuel Cycle*

ABSTRACT -NOTICE vepott was rnir . prepared as an accou.T of work S'Cut efponsibuity for the accuracy, completeness « uJulnas of any information, apparatus, product o process disclosed, or represents that its use would not d iht process disclo, p infringe privately owned lights. Aquatic organisms are exposed to radionuclides released to the environment during various steps of the nuclear fuel cycle. Routine releases from these processes are limited in compliance with technical specifications, requirements of federal regulations. These regulations reflect I.C.R.P. recommendations which are designed to provide an environment considered safe for man. It is generally accepted that aquatic organisms will not receive damaging external radiation doses in such environments; however, because of possible bioaccunulation of radionuclides there is concern that aquatic organisms might be adversely affected 'ay internal doses. The objectives of this paper are: (1) to estimate the radiation dose received by aquatic biota from the different processes and determine the major dose-contributing radionuclides, and (2) to assess the impact of estimated doses on aquatic biota. Dose estimates are made by using radionuclide concentration measured in the liquid effiuents of representative facilities. Where measurements of concentrations are rot available, predicted radioactive releases to the aquatic environment are used for dose calculations. Although radioactive releases from reactors used to generate electrical energy have received the most attention, and are the best documented, this evaluation indicates the potential for a greater radiation dose to aquatic biota from the nuclear fuel supply facilities (i.e., mining and milling).. The effects of chronic low-level radiation on aquatic organisms are discussed from somatic and genetic viewpoints. Based on the body of radiobiological evidence accumulated up to the present time, no significant deleterious effects are predicted for populations of aquatic organisms exposed to the estimated dose rates resulting from routine releases from conversion, enrichment, fabrication, reactors and reporcessing facilities. At the doses estimated for milling and mining operations it would be difficult to detect radiation effects on aquatic populations; however, the significance of such radiation exposures to aquatic populations cannot be fully evaluated without further research on effects of chronic low-level radiation

In planta expression of A. cellulolyticus Cel5A endocellulase reduces cell wall recalcitrance in tobacco and maize

The glycoside hydrolase family 5 endocellulase, E1 (Cel5A), from Acidothermus cellulolyticus was transformed into both Nicotiana tabacum and Zea mays with expression targeted to the cell wall under a constitutive promoter. Here we explore the possibility that in planta expression of endocellulases will allow these enzymes to access their substrates during cell wall construction, rendering cellulose more amenable to pretreatment and enzyme digestion. Tobacco and maize plants were healthy and developed normally compared with the wild type (WT). After thermochemical pretreatment and enzyme digestion, transformed plants were clearly more digestible than WT, requiring lower pretreatment severity to achieve comparable conversion levels. Furthermore, the decreased recalcitrance was not due to post-pretreatment residual E1 activity and could not be reproduced by the addition of exogenous E1 to the biomass prior to pretreatment, indicating that the expression of E1 during cell wall construction altered the inherent recalcitrance of the cell wall

Detecting new physics contributions to the D0-D0bar mixing through their effects on B decays

New physics effects may yield a detectable mass difference in the D0-D0bar system, Delta m_D. Here we show that this has an important impact on some B --> D decays. The effect involves a new source of CP violation, which arises from the interference between the phases in the B --> D decays and those in the D0-D0bar system. This interference is naturally large. New physics may well manifest itself through Delta m_D contributions to these B decays.Comment: 10 pages, Revtex, no figures. To appear in PR

A Search for Dark Matter Annihilation with the Whipple 10m Telescope

We present observations of the dwarf galaxies Draco and Ursa Minor, the local group galaxies M32 and M33, and the globular cluster M15 conducted with the Whipple 10m gamma-ray telescope to search for the gamma-ray signature of self-annihilating weakly interacting massive particles (WIMPs) which may constitute astrophysical dark matter (DM). We review the motivations for selecting these sources based on their unique astrophysical environments and report the results of the data analysis which produced upper limits on excess rate of gamma rays for each source. We consider models for the DM distribution in each source based on the available observational constraints and discuss possible scenarios for the enhancement of the gamma-ray luminosity. Limits on the thermally averaged product of the total self-annihilation cross section and velocity of the WIMP, , are derived using conservative estimates for the magnitude of the astrophysical contribution to the gamma-ray flux. Although these limits do not constrain predictions from the currently favored theoretical models of supersymmetry (SUSY), future observations with VERITAS will probe a larger region of the WIMP parameter phase space, and WIMP particle mass (m_\chi).Comment: 33 pages, 12 figures, accepted for publication in the Astrophysical Journa

Fermilab E791

Fermilab E791, a very high statistics charm particle experiment, recently completed its data taking at Fermilab's Tagged Photon Laboratory. Over 20 billion events were recorded through a loose transverse energy trigger and written to 8mm tape in the the 1991-92 fixed target run at Fermilab. This unprecedented data sample containing charm is being analysed on many-thousand MIP RISC computing farms set up at sites in the collaboration. A glimpse of the data taking and analysis effort is presented. We also show some preliminary results for common charm decay modes. Our present analysis indicates a very rich yield of over 200K reconstructed charm decays.Comment: 4 pages, 1 figure, LaTe