Apeitos, Angelos

Blaylock, Reginald B.

Lemus, Jason T.

Saillant, Eric

Sarkisian, Brie L.

English

Aquila Digital Community

The University of Southern MississippiThe Aquila Digital CommunityResearch Data11-18-2018An Intensive, Large-Scale Batch Culture System toProduce the Calanoid Copepod, Acartia tonsa:Appendix ABrie L. SarkisianUniversity of Southern Mississippi, Brie.Sarkisian@usm.eduJason T. LemusFish and Wildlife Research Institute, Florida, jason.lemus@myfwc.comAngelos ApeitosUniversity of Southern Mississippi, angelos.apeitos@usm.eduReginald B. BlaylockUniversity of Southern Mississippi, reg.blaylock@usm.eduEric SaillantUniversity of Southern Mississippi, Eric.Saillant@usm.eduFollow this and additional works at: https://aquila.usm.edu/datasetsPart of the Marine Biology CommonsThis Article is brought to you for free and open access by The Aquila Digital Community. It has been accepted for inclusion in Research Data by anauthorized administrator of The Aquila Digital Community. For more information, please contact Joshua.Cromwell@usm.edu.Recommended CitationSarkisian, Brie L.; Lemus, Jason T.; Apeitos, Angelos; Blaylock, Reginald B.; and Saillant, Eric, "An Intensive, Large-Scale BatchCulture System to Produce the Calanoid Copepod, Acartia tonsa: Appendix A" (2018). Research Data. 1.https://aquila.usm.edu/datasets/11  Supplementary appendices to accompany: 1 Sarkisian, B. L., Lemus, J.T., Apeitos, A., Blaylock, R.B., and Saillant, E. A. 2018. An intensive, 2 large-scale batch culture system to produce the calanoid copepod, Acartia tonsa. Aquaculture 3 DOI: 10.1016/j.aquaculture.2018.11.042 4 Appendix A. Microalgae culture      5 Algae production is carried out in a batch culture system in a room adjacent to the 6 copepod production system.  A batch algae system is the most cost-effective method to provide 7 for high copepod demands and a small volume footprint (150 L, 6 trillion total cells day-1).  8 Tahitian–strain Isochrysis lutea, CCMP 1324 is used for the main source of algae to feed 9 copepod cultures.  Algae production is carried out in 19 L plastic bags set on racks with 10 fluorescent lights between the racks.  Light intensity is held at 12,000 – 17,000 lux by use of 32 11 watt, 2,900 lumen, 4,100 Kelvin mercury-fluorescent bulbs (GE 66349 - F32T8/SPP41/ECO, 12 General Electric, Boston, MA) over a 24-h photoperiod.  The bags are inoculated with 2 L of 13 algae (in exponential phase at a minimum density of 30 x 106 cells mL-1) and 16 L of seawater.  14 Bags are fed F/2 Algae Food (Fritz Aquatics, Mesquite, TX), 2.5 mL of part A to 2.5 mL part B 15 solution every other day.  After a ten to twelve day grow-out period, the bags are harvested as 16 feed for the copepods at a density of 40 - 50 x 106 cells mL-1. 17 For the years 2014 and 2015, T-Iso feedings were supplemented with Rhodomonas lens, 18 Pascher & Ruttner, 1913, CCMP 739 (Rhodo) was produced in a separate room.  This algae 19 requires a specific photoperiod (10 h light : 14 h dark cycle using the same techniques as for T-20 Iso), so it was cultured in another building.  Rhodo bags were inoculated and supplied with 21 nutrient media the same way as T-Iso, reaching a density of 15 - 20 x 106 cells mL-1.  On any 22 given day, only a total of 25 L of Rhodo (0.4 trillion total cells) was available to supplement 23 copepod feedings. 24 2   25 Appendix B.  Egg production from a single tank over three trials in 2013, 2014, and 2015.  Bars 26 represent an average of daily egg production over a seven-day collection period ± SD.  Average 27 daily egg production across years is represented by a curved line. The procedure to derive daily 28 production and averages is the same as that described in section 3.3 but the single tank was 29 counted separately.  30  31  32  33  34 

An Intensive, Large-Scale Batch Culture System to Produce the Calanoid Copepod, \u3ci\u3eAcartia tonsa\u3c/i\u3e: Appendix A

https://aquila.usm.edu/cgi/viewcontent.cgi?article=1001&amp;context=datasets

An Intensive, Large-Scale Batch Culture System to Produce the Calanoid Copepod, \u3ci\u3eAcartia tonsa\u3c/i\u3e: Appendix A

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