Akar Adventif Kedelai Teriradiasi pada Cekaman Genangan

Kedelai (Glycine max L.) merupakan komoditas pangan utama selain padi dan jagung yang mengandung protein, minyak, dan karbohidrat tinggi. Kebutuhan yang meningkat tidak diimbangi dengan peningkatan produksinya. Salah satunya karena pengaruh faktor cekaman genangan. Pada penelitian ini, induksi variasi dilakukan dengan metode iradiasi sinar Gamma dengan dosis penyinaran 25Gy, 50Gy, 75Gy, dan 100Gy. Varian hasil iradiasi kemudian diseleksi pada kondisi tergenang dengan konsentrasi air sebesar 100%, 150%, 200%, dan 250%. Tujuan dari penelitian ini adalah mengetahui pengaruh interaksi antrara cekaman genangan dan iradiasi terhadap jumlah akar adventif pada kedelai. Karakter morfologi yang diamati yang parameter jumlah akar adventif.. Parameter jumlah akar adventif mengalami peningkatan secara signifikan seiring dengan peningkatan konsentrasi penggenangan sedangkan untuk dosis iradiasi tidak memberikan pengaruh terhadap jumlah akar adventif sehingga dapat diketahui bahwa interaksi antara Iradiasi dan Genangan memberikan pengaruh yang tidak signifikan pada parameter akar adventif. Peningkatan jumlah akar adventif tertinggi terjadi pada genangan 250% dan iradiasi 75 Gy dengan nilai tertinggi 7 , sedangkan untuk rata-rata jumlah akar yang paling rendah dengan pemberian dosis iradiasi 25 Gy pada konsentrasi penggenangan 100%, yaitu 1

Cryo-thawed embryo transfer: natural versus artificial cycle. A non-inferiority trial.(ANTARCTICA trial)

Background: frozen thawed embryo transfer (FET) is a cost-effective adjunct to IVF or IVF-ICSI treatment. In order to optimize treatment outcome, FET should be carried out during a period of optimal endometrial receptivity. To optimize implantation several methods for endometrium preparation have been proposed. In natural cycle FET (NC-FET), the endometrium develops under endogenous hormonal stimulation. The development of the dominant follicle and endometrium is monitored by ultrasound and FET is timed after triggering ovulation induction or determination of the spontaneous LH surge. In an artificial cycle FET (AC-FET) estrogens and progesterone are administered to prepare the endometrium for implantation. While the currently available data show no significant difference in pregnancy rates between these methods, well designed randomized controlled trials are lacking. Moreover there is little literature on difference in cancellation rates, cost-efficiency and adverse events.Methods and design: this randomized, multi-centre, non-inferiority trial we aim to test the hypothesis that there is no significant difference in live birth rates between patients undergoing NC-FET versus AC-FET. The primary outcome will be live birth rate per embryo transfer procedure. Secondary outcomes will be ongoing and clinical pregnancy rate, cancellation rate, (serious) adverse events and cost-efficiency. Based on a live birth rate of 20% and a minimal clinical important difference of 7.5% (one-sided alpha 2.5%, beta 20%) a total of 1150 patients will be needed. Analyzes will be performed using both per protocol as well as intention to treat analyses.Discussion: this prospective, randomized, non-inferiority trial aims to address the hypothesis that there is no significant difference in live birth rates between patients undergoing NC-FET versus patients undergoing AC-FET. Moreover it addresses cost-efficiency as well as the perceived burden of both treatments. TRIAL REGISTER: Netherlands trial register (NTR): 1586