Integration of a virus membrane protein into the lipid bilayer of target cells as a prerequisite for immune cytolysis

Structural requirements for membrane antigens on target cells to mediate immune cytolysis were studied in a model system with purified membrane proteins from Semliki Forest virus (SFV). These SFV spike proteins were isolated in the form of detergent- and lipid-free protein micelles (29S complexes) or, after reconstitution into lipid vesicles, in the form of virosomes. Both the 29S complexes and the virosomes were found to bind well to murine tumor cells (P815 or Eb). When these cells, however, were used as target cells in complement-dependent lysis or in antibody-dependent cell- mediated cytotoxicity assays in the presence of anti-SFV serum, they were not lysed, although they effectively bound the antibody and consumed complement. The same tumor cells infected with SFV served as positive controls in both assays. Different results were obtained when inactivated Sendai virus was added as a fusion reagent to the cells coated with either virosomes or 29S complexes. Under these conditions the virosome-coated cells became susceptible to SFV- specific lysis, whereas the 29S complex-coated cells remained resistant. Evidence that the susceptibility to lysis ofvirosome-coated cells was dependent on active fusion and, therefore, integration of the viral antigens into the lipid bilayer of the target cells was derived from control experiments with enzyme-treated Sendai virus preparations. The 29S complexes and the virosomes partially and selectively blocked the target cell lysis by anti-H-2 sera but not by anti-non-H-2 sera confirming our previous finding that major histocompatibility antigens serve as receptors for SFV. The general significance of these findings for mechanisms of immune cytolysis is dicussed

Phi meson production in near threshold proton-nucleus collisions

The cross section for production of Phi mesons in proton-nucleus reactions is calculated as a function of the target mass. The decay width of the Phi meson is affected by the change of the masses of the Phi, K+ and K- mesons in the medium. A strong attractive K- potential leads to a measurable change of the behavior of the cross section as a function of of the target mass. Comparison between the kaon and electron decay modes are made.Comment: 4 pages, 1figure, new figure, new reference

Calculations of K+, K- and phi Production in Near-Threshold Proton-Nucleus Collisions

K+, K- and \phi meson production in proton-nucleus (pA) collisions has been calculated within a BUU transport model. It is shown that the nucleon-hyperon strangeness transfer channel is essential. The role of three-body reactions has been investigated within the medium. The targetmass dependence of $\phi$ production is predicted to give important information on the in-medium properties of all three mesons.Comment: Talk presented by H.W.B. at the Budapest 2004 workshop on 'Hot and Dense Matter in Relativistic Heavy Ion Collisions', March 24-27, 2004, Budapest, Hungar

Strangeness production time and the K+/pi+ horn

We construct a hadronic kinetic model which describes production of strange particles in ultrarelativistic nuclear collisions in the energy domain of SPS. We test this model on description of the sharp peak in the excitation function of multiplicity ratio K+/pi+ and demonstrate that hadronic model reproduces these data rather well. The model thus must be tested on other types of data in order to verify the hypothesis that deconfinement sets in at lowest SPS energies.Comment: proceedings of Hot Quarks 0

Subthreshold phi-meson production in heavy-ion collisions

Within a transport code of BUU type the production of phi-mesons in the reactions Ni+Ni at 1.93 AGeV and Ru+Ru at 1.69 AGeV is studied. New elementary reaction channels rho+N(Delta) to phi+N and pi+N(1520) to phi+N are included. In spite of a substantial increase of the \phi multiplicities by these channels the results stay below the tentative numbers extracted from experimental data.Comment: 17 pages(LaTeX), two new figures adde

Coulomb corrections to the three-body correlation function in high-energy heavy ion reactions

Starting from an asymptotically correct three-body Coulomb wave-function, we determine the effect of Coulomb final state interaction on the three-particle Bose-Einstein correlation function of similarly charged particles. We numerically estimate that the Riverside approximation is not precise enough to determine the three-body Coulomb correction factor in the correlation function, if the characteristic HBT radius parameter is 5 - 10 fm, which is the range of interest in high-energy heavy ion physics.Comment: 14 pages, 4 figure

Radial flow has little effect on clusterization at intermediate energies in the framework of the Lattice Gas Model

The Lattice Gas Model was extended to incorporate the effect of radial flow. Contrary to popular belief, radial flow has little effect on the clusterization process in intermediate energy heavy-ion collisions except adding an ordered motion to the particles in the fragmentation source. We compared the results from the lattice gas model with and without radial flow to experimental data. We found that charge yields from central collisions are not significantly affected by inclusion of any reasonable radial flow.Comment: 8 pages, 2 figures, submitted to PRC; Minor update and resubmitted to PR

Transport theories for heavy ion collisions in the 1 AGeV regime

We compare multiplicities as well as rapidity and transverse momentum distributions of protons, pions and kaons calculated within presently available transport approaches for heavy ion collisions around 1 AGeV. For this purpose, three reactions have been selected: Au+Au at 1 and 1.48 AGeV and Ni+Ni at 1.93 AGeV