The role of ADAMTS1 and versican in human myocardial infarction: A postmortem study

Objective: To determine the role of a disintegrin and metalloproteinase with thrombospondin type 1 motif (ADAMTS1) and fragmented versican in the myocardial infarction (MI) process in humans and to evaluate the diagnostic efficacy of ADAMTS1 for postmortem diagnosis of MI. Methods: Thirty autopsied individuals were allocated into 2 groups, namely, a study group of individuals who died of myocardial infarction (n = 20), and a control group who died of trauma (n = 10). We performed standard immunohistochemical staining on myocardial tissue specimens, studying anti-ADAMTS1, anti-versican, and anti-versican C terminal peptide sequence (DPEAAE) fragments. Results: Strong, diffuse staining was observed throughout myocardial tissue for ADAMTS1 in the 2 groups. However, in the study group, we observed no expression for ADAMTS1 around fibrotic areas but detected slight staining in coagulative and necrotic zones. Conclusion: Similar localizations of ADAMTS and fragmented versican in human heart tissue indicate that versican presumably is cleaved by ADAMTS1. Hence, ADAMTS1 can be regarded as a new marker for postmortem differential diagnosis of MI

Retrospective ınvestigation of ınpatients with urinary ınfection in an university medical center

Adnan Menderes Üniversitesi Tıp Fakültesi Arastırma ve Uygulama Hastanesi Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Klinigi'nde yatırılarak tedavi edilen üriner sistem infeksiyonlu hastalar geriye dönük olarak incelenerek tanı ve tedavi yaklasımlarınındegerlendirilmesi amaçlandı. Adnan Menderes Üniversitesi Tıp Fakültesi Arastırma ve Uygulama Hastanesi Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Klinigi'nde 01.01.200101.01.2009 tarihleri arasında yatırılarak tedavi edilen 73'ü kadın (%68,2), 34'ü erkek (%31,8) toplam 107 hasta geriye dönük olarak degerlendirildi. Hastalarda en sık rastlanılan belirtiler ates, dizüri ve kostavertebral açı hassasiyeti olarak saptandı. Alınan idrar kültürlerinde %68.2, kan kültürlerinde ise % 35.7 oranında üreme saptandı. Idrar ve kan kültürlerinde en sık üreyen mikroorganizma . Hastaların 79'unda (%74,7) tedaviye empirik olarak, diger hastalara ise (n=27 %25,3) kültür-antibiyogram sonucu ile baslanmıstı. Empirik tedavide birinci seçenek olarak seftriaksonun (n=65, %83) kullanıldıgı tespit edildi. Çalısmamızda tedaviye empirik olarak baslama oranının yüksek oldugu dikkatimizi çekmistir. Bu durumun düzeltilebilmesi için empirik tedavi baslama ölçütlerinin belirlenmesi önemlidir.It was aimed to evaluate the diagnostic and therapeutic approaches to urinary infections by retrospectively investigating the data belonged to inpatients with urinary infection treated in the clinic of Infectious Diseases and Clinical Microbiology ofAdnan Menderes University Medical Center. 107 patients (73 female and 34 male) treated in the Clinic of Infectious Diseases and Clinical Microbiology of Adnan Menderes University Medical Center between 01.01.2001 and 01.01.2009 were retrospectively investigated. The most commonly observed symptoms in the patients were fever, dysuria and costovertebral angle tenderness. Germinal reproduction was detected in the urinary and blood cultures in a ratio of 68.2% and 35.7%, respectively. was the most commonly detected microorganism in the blood and urine cultures. While 74.7 % of the patients (n=79) were treated by empiric antibiotics due to urgency, 25,3 % of the patients were treated based on culture and antibiogram results. Ceftriaxone was the first choice in the empiric treatment (n=65; 83%). We observed that the rate of starting to therapy empirically was higher. That's why it is important to determine the starting criteria of empiric treatment

A Rare Cause of Acute Abdomen: Perforation of Duodenal Diverticulum Containing Ectopic Pancreatic Tissue

Perforation is a rare but serious complication of a duodenal diverticulum and often presents itself with nonspecific symptoms and signs. Ectopic pancreatic tissue within a duodenal diverticulum is another rare situation. In this article, we report a case of an 87 year-old woman who presented with spontaneous perforation of the duodenal diverticulum. Operative resection and simple closure of the duodenum was performed. Ectopic pancreatic tissue was observed within the diverticulum at histological evaluation

Retrospective Evaluation of Twenty Seven Patients with Diabetic Foot Infection

Aim: This study analyzed diabetic foot infections (DFI) in patients who were followed up in our clinic. Methods: We retrospectively evaluated demographic characteristics and clinical and laboratory findings in 27 patients (12 female, 15 male) with DFI who were followed up in our clinic between June 2013 and January 2014. The foot ulcerations in the patients were classified according to Wagner’s classification. Results: The mean age of the patients was 59 years. Erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) and white blood cell levels were found to be high in 89%, 78% and only 22% of patients, respectively. 78% of patients had grade 2 and lower grade ulcerations. Growth of methicillin-resistant Staphylococcus aureus, Enterobacter cloacae and Enterococcus spp. was encountered in five (28%) of 18 patients in whom deep tissue culture test could be performed. Conclusion: Appropriate diagnosis and treatment planning can be achieved by guiding classifications performed by regarding clinical, neurological and vascular conditions as well as ulcer depth. In follow-up of cases, ESR and CRP tests are very useful in diagnosis and treatment of the complications such as osteomyelitis, which may lead to amputation. Treatment should be conducted with a multidisciplinary approach in tertiary healthcare facilities