984 research outputs found
Epigenetikai összetevők szerepe embrionális őssejtek differenciációjában = The role of epigenetic components in the differentiation of embrionic stem cells
Az elmĂşlt Ă©vek során a pályázat keretĂ©ben rĂ©szletesem vizsgáltuk, a PRMT1 Ă©s az arginin metiláciĂł szerepĂ©t az Ĺ‘ssejtek differenciáciĂłjában. Az elvĂ©gzett kĂsĂ©rletek szerint a PRMT1 hiányos egĂ©r embrionális Ĺ‘ssejtek bár eltĂ©rnek a vad tĂpusĂş Ĺ‘ssejtektĹ‘l, ez az eltĂ©rĂ©s nem jelenti a differenciáciĂłs kĂ©pessĂ©g eltĂĽnĂ©sĂ©t. Ez az eredmĂ©ny ellentmond az eredetileg megfoglamazott hipotĂ©zisĂĽnknek Ă©s nĂ©hány publikált adatnak is. Ennek ellenĂ©re kiterjedt Ă©s alapos vizsgálataink szerint a PRMT1 hiányos sejtek kĂ©pesek a három csĂra vonal sejtdifferenciáciĂłs programjait vĂ©gigjárni. EredmĂ©nyeink alapján a fehĂ©rjĂ©k arginin metiláciĂłja PRMT1 hiányában jelentĹ‘sen csökken Ă©s ez a hiány sejtszintű zavarokhoz vezethet (pl sejt migracio). Ez okozhatja a PRMT1 hiányos embrio pusztulását. | During our studies we analysed in details the role of PRMT1 and arginine methylation in the differentiation of mouse embryonic stem cells. Based on the performed experiments we can conclude that, although PRMT1 null mouse embryonic stem cells show dstinct features from wild type embryonic stem cells, their differentiation capacity is not changed signifficantly. This result is contradicting our original hypothesis and some of the published data in the literature. Our results show that PRMT1 null cells can differentiate along all the three differentiation pathways. The methylation of proteins is significantly altered by the silencing of PRMT1 and this might cause changes on cellular level (e.g. migration). This effects might be responsible for the deat of PRMT1 null embryos
Magreceptorok működésének genomszintü vizsgálata kromatin immunprecipitációval primer humán immun sejtekben = Decoding nuclear hormone receptor activity using chromatin immunoprecipitation in human primary immune cells
A kutatĂłmunka során szisztematikusan feltĂ©rkĂ©peztĂĽk az un RXR heterodimer tĂpusĂş receptorok szerepĂ©t a humán monocita eredetű dendritikus sejtek differenciálĂłdása Ă©s immunfunkciĂłi során. Ezek a receptorok a PPARg, LXR, VDR Ă©s RAR receptorok volta, melyeket kiegĂ©szĂtettĂĽnk az RXR receptorral is. A specikus utvonalak azonosĂtása mellett általános megállapĂtáskĂ©nt elmondhattuk, hogy ez a receptorcsalad olyan molekuláris szenzorkĂ©nt működik, mely kĂ©pes átprogramozni a sejt genkifejezĹ‘dĂ©sĂ©t kĂĽlsĹ‘ Ă©s belsĹ‘ lipid szintek változása során. Ă–sszefĂĽggĂ©st talaltunk az IL4 STAT6-on kerestĂĽli szignalizáciĂłja Ă©s a PPARg receptor között Ă©s legĂşjabban feltĂ©rkĂ©peztĂĽk a az RXR receptor genomi kötĹ‘helyeit ls cisztromikus kölcsönhatásait is. | During our work we have systematically mapped the so called RXR heterodimeric receptors roles in monocyte derived dendritic cells during differentiation and immune function. These receptors included PPARg, LXR, VDR and RXR and also included their heterodimeric partner RXR. Based on the data obtained one can conclude that besides the specialized pathways identified, these receptors act as molecular sensors in detecting changing extra and intracellular lipid levels. We have also identified an interaction between IL4 mediated STAT6 signaling and PPARg and most recently we have detrained the RXR cistrome and its interactions
Effect of imidazole and indomethacin on hemodynamics of the obstructed canine kidney
Effect of imidazole and indomethacin on hemodynamics of the obstructed canine kidney. In the anesthetized dog renal blood flow (RBF) and its intrarenal distribution were investigated by the radioactive microsphere technique 24hr after bilateral (BUL) and unilateral (UUL) ureteral ligation. In the control series indomethacin (IM) led to a decrease in RBF with outward shifting of zonal perfusions; imidazole (IA) did not cause significant changes in renal hemodynamics. In the BUL series there was a sharp drop in RBF with a proportional decrease in outer (OC) and inner (IC) cortical perfusion; IM treatment resulted in a further decrease in overall and zonal perfusions. I A, a selective inhibitor of thromboxane synthetase, relieved IC vasoconstriction. In the ligated kidney of the UUL preparations decrease in RBF was due to OC vasoconstriction, while IC perfusion equalled controls. IM led to an overall vasoconstriction in all cortical layers; IA did not influence either total RBF or its distribution. It was concluded that BUL “unmasked” TXA2 production in the IC layers, while IM treatment, by inhibiting the production of PGE2, PGI2, and TXA2, resulted in an overall vasoconstriction both in controls and the BUL and UUL preparations.Effet de l'imidazole et de l'indométhacine sur l'hémodynamique du rein de chien en obstruction. Chez le chien anesthésié le flux sanguin rénal (RBF) et sa distribution intrarénale ont été étudiés par la technique des microsphères radioactives 24hr après ligature urétérale bilatérale (BUL) ou unilatérale (UUL). Dans la série contrôle, l'indométhacine (IM) diminuait RBF avec un déplacement des perfusions régionales vers l'extérieur; l'imidazole (IA) ne modifiait pas significativement l'hémodynamique rénale. Dans la série BUL, il y avait une chute prononcée de RBF avec une baisse proportionnelle de la perfusion corticale externe (OC) et interne (IC); le traitement IM diminuait encore plus les perfusions globales et régionales. IA, un inhibiteur sélectif de la thromboxane synthétase, diminuait la vasoconstriction IL. Dans le rein ligaturé des préparations UUL, la baisse de RBF était due à une vasoconstriction OC, alors que la perfusion IC était égale à celle des contrôles. IM entraînait une vasoconstriction globale dans toutes les couches corticales; IA n'influençait ni RBF total, ni sa distribution. On a conclu que BUL “démasquait” une production de TXA2 dans les couches IC, tandis que le traitement IM, en inhibant la production de PGE2, PGI2, et TXA2, entraînait une vasoconstriction globale dans les préparations contrôles, BUL et UUL
Identification and Spectral Evaluation of Agricultural Crops on Hyperspectral Airborne Data
Hyperspectral remote sensing combined with advanced image processing techniques is an efficient tool for the identification of agricultural crops. In our study we pursued spectral analysis on a relatively small sample area using low number of training points to examine the potential of high resolution imagery. Spectral separability measurements were applied to reveal spectral overlapping between 4 crop species and for the discrimination we also used statistical comparisons such as plotting the PC values and calculating standard deviation of single band reflectance values on our classes. These statistical results were proven to be good indicators of spectral similarity and potential confusion of data samples. The classification of Spectral Angle Mapper (SAM) had an overall accuracy of 72% for the four species where the poorest results were obtained from the test points of garlic and sugar beet. Comparing the statistical analyses we concluded that spectral homogeneity does not necessarily have influence on the accuracy of mapping, whereas separability scores strongly correlate with classification results, implying also that preliminary statistical assessments can improve the efficiency of training site selection and provide useful information to specify some technical requirements of airborne hyperspectral surveys
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