Heterocyclic compounds degradation and characterization of Antarctic psychrophilic bacteria strain BS1

The Tenth Symposium on Polar Science/Ordinary sessions : [OB] Polar Biology, Wed. 4 Dec. / Entrance Hall (1st floor) , National Institute of Polar Researc

Isolation and heterocyclic compounds degradation of Antarctic psychrophilic bacteria strain BS19

The Tenth Symposium on Polar Science/Ordinary sessions : [OB] Polar Biology, Wed. 4 Dec. / Entrance Hall (1st floor) , National Institute of Polar Researc

Comparison between Double Stranded DNA with Restriction Enzymes and Single Stranded DNA with Primers for Solving Boolean Matrix Multiplication

Boolean matrix multiplication is the basis for most computing algorithms and is widely used in many fields.  In this paper, we compare and discuss two methods to solve Boolean matrix multiplication with DNA computing.  The first method utilizes double stranded DNA sequences with Restriction Enzymes meanwhile the second method utilizes single stranded DNA sequences with primers.  We prove that while both methods are able to solve the Boolean matrix multiplication problem, these two methods differ in their performance and output results.  We compare the advantages of the latter method in terms of easier sequence designs and more efficient analysis of results

Detection and isolation of genes involved in heterocyclic compounds degradation from Antarctic psychrophilic bacteria

The Tenth Symposium on Polar Science/Ordinary sessions : [OB] Polar Biology, Wed. 4 Dec. / Entrance Hall (1st floor) , National Institute of Polar Researc

Optimisation for Enhancement of Phenol Degradation by Arthrobacter sp. Strain AQ5-15 from Antarctic Soil Using Conventional and Statistical Approach

The Tenth Symposium on Polar Science/Ordinary sessions : [OB] Polar Biology, Wed. 4 Dec. / Entrance Hall (1st floor) , National Institute of Polar Researc

Comparison between Double Stranded DNA with Restriction Enzymes and Single Stranded DNA with Primers for Solving Boolean Matrix Multiplication

Boolean matrix multiplication is the basis for most computing algorithms and is widely used in many fields. In this paper, we compare and discuss two methods to solve Boolean matrix multiplication with DNA computing. The first method utilizes double stranded DNA sequences with Restriction Enzymes meanwhile the second method utilizes single stranded DNA sequences with primers. We prove that while both methods are able to solve the Boolean matrix multiplication problem, these two methods differ in their performance and output results. We compare the advantages of the latter method in terms of easier sequence designs and more efficient analysis of results

Psychrotolerant biosurfactant-producing bacteria for hydrocarbon degradation: a mini review

Biosurfactants are a structurally diverse group of surface-active substances synthesised by microorganisms. All biosurfactants have tremendous potential ranging from medicine to environmental applications especially in hydrocarbon remediation. Petroleum pollution is a major issue in both cold and temperate climate countries. These hydrocarbon pollutants have low solubility and high solid-water distribution ratios, thus limiting the interaction between microbial cells. Petroleum pollution is a major issue in both cold and temperate climate countries. In Antarctica, due to the recalcitrant nature of hydrocarbon components coupled with the region's extremely weather conditions, there were difficulties faced by bioremediation approaches. However, using biosurfactant in hydrocarbon bioremediation increases the bioavailability of hydrocarbon, thus expediting bioremediation. Few studies have reported on psychrotolerant bacterial species that are able to degrade hydrocarbon and produce biosurfactants. This review focuses on psychrotolerant bacteria with the potential to synthesise biosurfactants and degrade hydrocarbons

Decolourisation Capabilities of Ligninolytic Enzymes Produced by Marasmius cladophyllus UMAS MS8 on Remazol Brilliant Blue R and Other Azo Dyes

Marasmius cladophyllus was examined for its ability to degradatively decolourise the recalcitrant dye Remazol Brilliant Blue R (RBBR) and screened for the production of ligninolytic enzymes using specific substrates. Monitoring dye decolourisation by the decrease in absorbance ratio of A 592/A 500 shows that the decolourisation of RBBR dye was associated with the dye degradation. Marasmius cladophyllus produces laccase and lignin peroxidase in glucose minimal liquid medium containing RBBR. Both enzyme activities were increased, with laccase activity recorded 70 times higher reaching up to 390 U L-1 on day 12. Further in vitro RBBR dye decolourisation using the culture medium shows that laccase activity was correlated with the dye decolourisation. Fresh RBBR dye continuously supplemented into the decolourised culture medium was further decolourised much faster in the subsequent round of the RBBR dye decolourisation. In vitro dye decolourisation using the crude laccase not only decolourised 76% of RBBR dye in just 19 hours but also decolourised 54% of Orange G and 33% of Congo red at the same period of time without the use of any exogenous mediator. This rapid dye decolourisation ability of the enzymes produced by M. cladophyllus thus suggested its possible application in the bioremediation of dye containing wastewate

Enzymatic retting of Piper nigrum L. using commercial Pectinase(Peelzyme)

White peppers produced from Piper nigrum L. retted with different concentration of commercial pectinase (PeelZyme) and blanching treatment in hot water were compared. The effects of these treatments on surface morphology and piperine content of white berries was studied. PeelZyme at the concentration of 500 ppm successfully produced white berries after 5 days. However, white berries retted with PeelZyme at the concentration of 500 ppm without blanching gave the best surface morphology but there was a reduction in the piperine content by 3.04%. Blanching in hot water resulted in reduction of surface quality but an increase of piperine content up to 40% was obtained

Detection of Beneficial Lactic Acid Bacteria (LAB) and Yeast In Sarawak Fermented Food

Sarawak native’s fermented food can be a catalyst for boosting the local economy in Sarawak. The Lactic Acid Bacteria (LAB) are generally regarded as safe, have a stability of usage, and originate from natural resources. Lactic acid bacteria and yeast work in synergy to provide a natural way to enhance the nutritive value and flavour of the food. The study aims to investigate the presence of potential probiotic Lactic Acid Bacteria (LAB) and yeast isolated from Sarawak fermented food. Two hundred fifty (n=250) of samples including fifty (n=50) each sample such as fermented shrimps (cencaluk), fermented mustard vegetables (kasam ensabii), fermented fish (kasam ikan), fermented dabai (Canarium odontophyllum) and fermented fish (rusip). Molecular identification of the bacteria and yeast isolates was carried out by PCR amplification of the 16S rRNA (27F and 1492-R) and ITS (ITS5-F and ITS4-R) rRNA region. The successfully amplified PCR products were sent for Sanger sequencing As a result, a total of 45.2% (113/250) Lactic Acid Bcateria (LAB) which are 96% (48/50) of W. paramesenteroides was detected in fermented dabai, 80% (40/50) S. pasteuri in fermented fish rusip samples and 50% (25/50) in P. agglomerans in cencaluk samples. Meanwhile, yeast Candida species; 90% (45/50) of C. magnoliae and 50% (25/50) of C. parapsilosis are detected in fermented dabai and ensabi respectively. Based on the findings, both yeast and bacteriocin-producing lactic acid bacteria found in the fermented food specifically in dabai. A better understanding of microbial ecology can help the food industry to improve the foods in terms of quality and safety. The good quality of the LAB and yeast in the food such as starter culture will enhance the texture and nutritional value and Sarawak fermented food product found enriched with LAB