The Tenth Symposium on Polar Science/Ordinary sessions : [OB] Polar Biology, Wed. 4 Dec. / Entrance Hall (1st floor) , National Institute of Polar Researc

Kavilasni Subramaniam

Khalil Khalilah Abdul

Noor Azmi Shaharuddin

Siti Aqlima Ahmad

Zulkharnain Azham

National Institute of Polar Research Repository

  Optimisation for Enhancement of Phenol Degradation by Arthrobacter sp. Strain AQ5-15 from Antarctic Soil Using Conventional and Statistical Approach  Kavilasni Subramaniam1, Noor Azmi Shaharuddin1, Azham Zulkharnain3, Khalilah Abdul Khalil4 and Siti Aqlima Ahmad1,2 1 Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia. 2 National Antarctic Research Centre, B303 Level 3, Block B, IPS Building, Universiti Malaya, 50603 Kuala Lumpur, Malaysia. 3 Department of Bioscience and Engineering, College of Systems Engineering and Science, Shibaura Institute Technology, 307 Fukasaku, Minuma-ku, Saitama, 337-8570, Japan. 4 Biomolecular Science Program, School of Biology, Faculty of Applied Sciences, Universiti Teknology MARA, 40450, Shah Alam, Selangor, Malaysia  Phenol contamination becomes one of the most prevalent environmental pollution which should be taken into immediate consideration as they may cause negative impact on aquatic lives, plants and also human beings. This scenario can also be witnessed in the continent which once considered to be the most pristine and isolated continent, Antarctica. The continent’s cold and harsh climate resulting in the natural processes that help remediate pollution in other parts of the world happen relatively slow, hence, pollutant like phenol has more of a chance to bioaccumulate in the Antarctica’s simple ecosystem and most particularly, food chain. Therefore, actions of bioremediation are significant to overcome this problem. Although previous studies have reported the use of native microorganisms in phenol degradation, however, retrieving the eligible microbial population alone for the degradation of stated pollutant is not sufficient where enhancement of conditions is required for the viability of the bioremediation process. This study has revealed that bacteria Arthrobacter sp. strain AQ5-15 isolated from Antarctic soil was able to degrade phenol at low temperature. Study on the effects of significant factors including pH and temperature was carried out to optimise the conditions for phenol degradation. Finding revealed that this strain is psychrotolerant with optimum temperature at 20°C and prefer neutral or near-neutral condition for optimum phenol degradation. These conditions were further enhanced using response surface methodology approach which resulted in a better degradation with 99.42% compared to conventional one-factor-at-time approach with only 88.80% of degradation.             References Lee, G. L. Y., Ahmad, S. A., Yasid, N. A., Zulkharnain, A., Convey, P., Wan Johari, W. L., and Shukor, M. Y., Biodegradation of phenol by cold-adapted bacteria from Antarctic soils, Polar Biology, 41(3), 553–562, 2018.  Nawawi, N. M., Ahmad, S. A., Shukor, M. Y., Syed, M. A., Khalil, K. A., Rahman, N. A. A., Dahalan, F.A., Ibrahim, A. L., Statistical optimisation for improvement of phenol degradation by Rhodococcus sp. NAM 81, Journal of Environmental Biology; Lucknow, 37(3), 443–451, 2016. 

Optimisation for Enhancement of Phenol Degradation by Arthrobacter sp. Strain AQ5-15 from Antarctic Soil Using Conventional and Statistical Approach

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Optimisation for Enhancement of Phenol Degradation by Arthrobacter sp. Strain AQ5-15 from Antarctic Soil Using Conventional and Statistical Approach

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