O filme PARIS JE T´AIME : inquietações baudelaireanas, flâneurie & conflitos multiculturais

Este artigo investiga a cidade representada no filme Paris je t´aime e suas relações com a experiência urbana real, com base nos conceitos de Charles Baudelaire sobre flâneurie, solidão e incomunicabilidade. O propósito é discutir alguns dos principais problemas que tem lugar nas metrópoles pós-modernas, enfatizando conflitos étnicos e multiculturais. O artigo também analisa a oposição entre os episódios do filme e o imaginário ocidental sobre Paris como um fascinante locus utopicus - um lugar mágico para o amor

Atividade física e hábitos alimentares de participantes e não participantes do programa atleta do futuro (PAF)

O estudo objetivou investigar os hábitos de atividade física (AF) e o consumo alimentar de participantes e não participantes do programa Atleta do Futuro do SESI-PAF. Materiais e métodos: Participaram 494 escolares, sendo 246 do PAF (129 meninos e 117 meninas) e 248 não participantes do PAF (112 meninos e 136 meninas), com média de idade de 8 anos. Utilizou-se um questionário auto respondido dividido em informações gerais, estrato econômico, hábitos alimentares e AF. Para a comparação das informações gerais e estrato econômico entre os grupos utilizou-se o teste Qui quadrado. Comparação do nível de AF, consumo diário e gosto alimentar utilizou-se o teste U-Mann Whitney, com o nível de significância estatística de 95%. Resultados e discussão: A maioria dos responsáveis pelos escolares possui ensino médio e pertence ao estrato econômico B. A maioria dos participantes do PAF pratica atividades moderadas (58,5%) enquanto que as não participantes atividades leves (51,6%). Não foram encontradas diferenças significativas entre os grupos quanto ao consumo alimentar (ingesta de feijão, cereais, lacticínios, frutas e verduras, proteínas, e batata frita/pizza). Ambos os grupos gostam muito de frutas e sucos, refrigerantes, cereais e verduras, embora não consumam com frequência refrigerantes e guloseimas. Conclusão: Os participantes do PAF tendem a realizar AF de maior intensidade quando comparados aos não participantes. Além disso, o consumo alimentar dos participantes é semelhante nos dois grupos. Ressalta-se que o consumo inadequado de alimentos e a baixa intensidade da AF contribuem para o sobrepeso e a obesidade. ABSTRACT Physical activity and eating habits of participants and non-participants of the athlete of the future-PAFThis study aims to analyze the physical activity (PA) habits and the food intake of participants and non-participants of the program SESI Atleta do Futuro-PAF. 494 schoolchildren participated, being 246 of PAF (129 boys and 117 girls) and 248 non-participants of PAF (112 boys and 136 girls) with average 8 years of age. The instrument used in the study was an auto answered questionnaire composed of: General information, socioeconomic status, eating habits and PA. For comparison, the general information and socioeconomic among the groups the test used chi square and for comparison the level of physical activity, daily food consumption and like food the test used U-Mann Whitney, with level of statistical significance of 95%. Results and discussion: Most of the school children’s parents attended secondary school and belong to socioeconomic stratum B. The assessment of PA indicated that the participants of PAF practiced moderate-intensity activities (58,5%) and the non-participants performed light-intensity activities (51,6%). No found differences significant among the groups in food consumption (intake bean, grain products, dairy products, fruit and vegetables, protein and French fries/pizza. Both groups really like fruit and juice, soft drink, grain products and vegetables, although do not consume soft drink and candies. Conclusion: Although the absence difference significant, the participants PAF tend to be done PA of higher intensity when compared to non-participants. Furthermore, the habits food intake of participants it is similar in both groups. It is noteworthy that the inadequate food intake and low intensity of PA contribute to overweight and obesity

Postharvest conservation of strawberry fruits at different storage conditions

O morango ? um produto muito apreciado pelos consumidores devido ?s caracter?sticas organol?pticas. Por?m, os frutos do morangueiro s?o altamente perec?veis depois de colhidos, sendo necess?rio adotar medidas alternativas para prolongar o per?odo de conserva??o dos frutos. O trabalho foi realizado com o objetivo de avaliar a conserva??o p?s-colheita de frutos de cultivares de morangueiro em diferentes condi??es de armazenamento. Os frutos de morangueiro utilizados no experimento foram cultivados na fazenda da empresa Mape Frutas, localizada no munic?pio de Datas-MG. Foram avaliados os frutos de oito cultivares (Festival, Campinas, Toyonoka, Dover, Oso Grande, Camarosa Diamante e Aromas). As condi??es de armazenamento e as an?lises foram realizadas no Laborat?rio de Tecnologia Biomassa do Cerrado da UFVJM. As condi??es de armazenamento foram em c?mara fria (temperatura m?dia 2,34?0,78?C e umidade relativa 89,93?4,14%) e condi??es ambiente (temperatura m?dia 17,43?2,68?C e umidade relativa 74,11?10,44%). As caracter?sticas foram analisadas por 12 dias, com avalia??es a cada 3 dias: apar?ncia, incid?ncia de doen?as, teores de s?lidos sol?veis totais, acidez titul?vel total, vitamina C e firmeza. O delineamento experimental utilizado foi em blocos casualizados, em esquema fatorial com oito cultivares de morangueiro e cinco tempos de armazenamento, com tr?s repeti??es, avaliados separadamente nas duas condi??es de armazenamento. Em condi??es ambiente os frutos de morangueiro podem ser armazenados por no m?ximo tr?s dias. O armazenamento em c?mara fria proporciona maior conserva??o p?s-colheita de frutos de morangueiro, podendo os frutos ser armazenados at? doze dias. A cv. Festival apresentou melhor conserva??o p?s-colheita nas duas condi??es de armazenamento, enquanto que as cvs. Toyonoka e Campinas apresentaram maior incid?ncia de doen?as e menor firmeza de frutos quando comparadas com as outras cultivares.Strawberry is a product appreciated by consumers due to the organoleptic characteristics. However, strawberry fruits are highly perishable after harvest, so alternative measures to prolong the shelf life of fruits are necessary. The objective of this experiment was to evaluate the fruit postharvest conservation of strawberry cultivars under different storage conditions. Strawberry fruits of eight cultivars (Festival, Campinas, Toyonoka, Dover, Oso Grande, Camarosa, Diamante and Aromas) were grown at ?Mape Frutas? farm, in Datas municipality, Minas Gerais State, Brazil. Strawberry fruits were stored at cold storage (2.34?0.78?C; 89.93?4.14% RH) and environmental conditions (17.43?2.68?C; 74.11?10.44%). The experimental design was randomized blocks in a factorial arrangement with eight strawberry cultivars and five storage times, with three replications. Fruits appearance, disease incidence, total soluble solids, titratable acidity, vitamin C and firmness were evaluated every 3 days, until 12 days. At room condition, strawberry fruit could be stored for only three days. Fruits kept in cold chamber kept quality until 12 days. Cv. Festival had the best postharvest shelf life in both storage conditions and cvs. Toyonoka and Campinas showed higher incidence of diseases and less fruit firmness when compared to the other cultivars

Evaluation of immunoglobulin purification methods and their impact on quality and yield of antigen-specific antibodies

<p>Abstract</p> <p>Background</p> <p>Antibodies are the main effectors against malaria blood-stage parasites. Evaluation of functional activities in immune sera from Phase 2a/b vaccine trials may provide invaluable information in the search for immune correlates of protection. However, the presence of anti-malarial-drugs, improper collection/storage conditions or concomitant immune responses against other pathogens can contribute to non-specific anti-parasite activities when the sera/plasma are tested <it>in vitro</it>. Purification of immunoglobulin is a standard approach for reducing such non-specific background activities, but the purification method itself can alter the quality and yield of recovered Ag-specific antibodies.</p> <p>Methods</p> <p>To address this concern, various immunoglobulin (Ig) purification methods (protein G Sepharose, protein A/G Sepharose, polyethylene glycol and caprylic acid-ammonium sulphate precipitation) were evaluated for their impact on the quality, quantity and functional activity of purified rabbit and human Igs. The recovered Igs were analysed for yield and purity by SDS-PAGE, for quality by Ag-specific ELISAs (determining changes in titer, avidity and isotype distribution) and for functional activity by <it>in vitro </it>parasite growth inhibition assay (GIA).</p> <p>Results</p> <p>This comparison demonstrated that overall polyethylene glycol purification of human serum/plasma samples and protein G Sepharose purification of rabbit sera are optimal for recovering functional Ag-specific antibodies.</p> <p>Conclusion</p> <p>Consequently, critical consideration of the purification method is required to avoid selecting non-representative populations of recovered Ig, which could influence interpretations of vaccine efficacy, or affect the search for immune correlates of protection.</p

Life experiences throughout the ifespan: What do people say (or not) about them?

Life experiences have been a topic of interest for researchers and clinicians for decades. Current knowledge is rooted in two distinct approaches, i.e., personality psychology and psychosomatics. Whereas the first is interested in ordinary life stories of nonclinical individuals, based on a more qualitative, in-depth, and person-driven approach, psychosomatics stresses negative events, mainly in clinical samples, and presents a more quantitative, general, and construct-driven approach. Consequently, available evidence is dispersed and unrelated and many basic questions remain unanswered. This study aimed to explore occurrence, developmental stage, valence, and impact of life experiences and to analyze critical answering patterns (i.e., “I don’t remember,” missingness). Through a cross-sectional retrospective design, 394 adults from the community answered the Lifetime Experiences Scale, which covers 75 life experiences organized in eight domains (i.e., school, job, health, leisure, living conditions, adverse experiences, achievements, and people and relationships). Occurrence of life experiences varied greatly, and the mean number of experiences reported was approximately 30. Regarding developmental stage, most experiences were reported in just one stage—mainly adulthood—however, some could be considered chronic. Globally, life experiences tended to be clearly rated as positive or as negative; additionally, assessed experiences were mainly appraised as positive. Moreover, participants presented their experiences as significant, rating them as high impact. Overall, critical answering patterns were not very expressive: “I don’t remember” and missing answers were below 2 and 5%, respectively, in the majority of experiences. These findings offer several important new insights, suggesting that life experiences are mainly an idiosyncratic topic.This manuscript is part of a doctoral dissertation, which had the support of the Portuguese Foundation for Science and Technology (FCT), through the PhD grant with the reference SFRH/ BD/76022/2011, funded by POPH-QREN-Typology 4.1-Advanced Training, reimbursed by the European Social Fund and national funds from State Budget. This study was conducted at Psychology Research Centre (UID/PSI/01662/2013), University of Minho, and supported by the Portuguese Foundation for Science and Technology and the Portuguese Ministry of Education and Science through national funds and co-financed by FEDER through COMPETE2020 under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007653).info:eu-repo/semantics/publishedVersio

Genetic divergence is not the same as phenotypic divergence

Far too often, phenotypic divergence has been misinterpreted as genetic divergence, and based on phenotypic divergence, genetic divergence has been indicated. We have attempted to disprove this statement and call for the differentiation of phenotypic and genotypic variation