Optimization of Agriculture Land Use Development Basing on Natural-resource Potential (On the Example of the Stavropol Territory)

Natural resources potential of the territory and natural-historical mechanism of its development are pacing factors in agriculture differentiation and AIC-development. Key branches in the Stavropol Territory are crop production, sheep breeding and meat and milk cattle breeding. To meet the market demands “equating” differentiation of agricultural enterprises is implemented. Thus, in Stavropol Territory crop lands have been increased for crops required in the market that disbalanced the crop rotation, disturbed agro technologies and resulted in reduction in yields and grain quality. In the Territory 70 % of tillage is in risky agriculture. About 65 % of crop production (wheat, barley and others) are in draughty areas where out of 141 years (1861–2002) 41 % draughty, which determine high natural-climatic dependence of grain economy [1]. Annual and areal variability of grain production is also determined by topsoil heterogeneity and variety of relief. At present production of the basic market crop – winter wheat, which takes 75 % of acreage for cereals and leguminous crops, the productivity of the cultivated crop is 22.4–39.5 centner/hectare. It results in top soil changes such as compaction, fertilizer impoverishment and organic matter content (organic matter deficit is 400–700 kg/ha). Intensification of agrarian production results in quicker erosion processes, soil properties decline and lower fertility. The total area of eroded lands in the Territory is 1792000 ha [2]. That is why it is important to develop a new model of the economic use of Stavropol Territory. The main methods of study are analysis of the current status and organization of the agricultural zones of the Territory. The data were preceded with the software Statistica

Comprehensive statistical and bioinformatics analysis in the deciphering of putative mechanisms by which lipid-associated GWAS loci contribute to coronary artery disease

The study was designed to evaluate putative mechanisms by which lipid-associated loci identified by genome-wide association studies (GWAS) are involved in the molecular pathogenesis of coronary artery disease (CAD) using a comprehensive statistical and bioinformatics analysis

Sorption characteristics of economically viable silicate sorbents for sequestration of lead ions from aqueous solutions

The adsorption of Pb2+ from aqueous solutions by sorbents based on calcium silicates, obtained in multicomponent systems CaCl2-Na2SiO3-H2O (sorbent I) and CaSO4·2H2O-SiO2·nH2O-KOH-H2O (sorbent II), was studied. Surface area of the two sorbents was found to be 100 and 40 m2/g respectively. The sorption capacities of the adsorbent materials were found to be 3.6 l/mmol and 8.4 l/mmol respectively. Characterization of the sorbent materials was carried out. The equilibrium data was fitted in Langmuir's isotherm and the adsorption capacity of the adsorbent materials was determined. The distribution coefficients at Pb2+ ions for the sorbents were determined at different ratios of solid and liquid phases

The analysis of the prevalence of extragenital pathology in infertile women with external genital endometriosis

Background. External genital endometriosis (EGE) is often the cause of infertility, while coagulation of its foci leads to the restoration of fertility. Often, non-invasivepreoperative diagnosis of EGE is difficult. Today, the issue of laparoscopy in infertility and whether there is a need for additional indications for its implementation remains controversial. Identification of the relationship between certain extragenital diseases with EGE is promising in terms of both understanding the pathogenesis of EGE and predicting its development.The aim. Identification of the relationship of extragenital diseases with the presence of EGE in infertile women of reproductive age.Materials and methods. In preparation for assisted reproductive technology programs at the Vitromed Clinic, 1 132 women of reproductive age were examined from 2019 to 2021. Collection and processing of clinical and anamnestic data were carried out. The diagnosis of EGE was verified by laparoscopy with histological examination. The results were analyzed using exact test and binary logistic regression to build a model of the relationship between EGE and somatic pathology.Results. EGE was detected in 222 patients. A number of somatic diseases were found that are most common in EGE: bowel diseases; minor cardiac maldevelopments; chronic anemia; pathology of the pancreas; chronic tonsillitis; allergy. A logistic regression model was constructed to predict the presence of EGE in patients with the indicated somatic pathology with a sensitivity of 81.53 % and a specificity of 97.25 %.Conclusion. Identification of comorbid extragenital diseases associated with EGE in women with infertility can serve as a basis for expanding the scope of therapeutic and diagnostic measures, in particular for laparoscopy

ACTIVITY OF FREE RADICAL OXIDATION AND CYCLOOXYGENASE EXPRESSION IN RATS KIDNEYS UNDER EXPERIMENTAL THERAPY OF OXALATE NEPHROLITHIASIS WITH TETRAPEPTIDE Leu-Ile-Lys-Met

The aim of the study was to investigate the effect of Leu-Ile-Lys-Met tetrapeptide on the activity of free radical oxidation (FRO) and the level of cyclooxygenase-1 (COX-1) and COX-2 expression in rat kidney with experimental oxalate nephrolithiasis. Material and methods. The experiments were carried out on 30 male rats Wistar runoff, divided into 3 groups: the comparison group (10 intact rats), the control group (6 weeks of experimental oxalate nephrolithiasis simulation, n = 10), the experimental group (6 weeks of experimental simulation oxalate nephrolithiasis + oral administration of Leu-Ile-Lys-Met tetrapeptide at a dose of 12 mg/kg from the 3rd to the 6th week, n = 10). The oxalate nephrolithiasis was simulated according to conventional ethylene glycol model. COX-1 and COX-2 concentration in rat urine at baseline and after 6 weeks of experimental nephrolithiasis was determined by enzyme immunoassay using the prostaglandin-endoperoxide synthase 1 and 2 (PTGS 1, 2) kit from «Cloud-Clone Corp.». In rat kidney homogenate, the activity indicators of FRO processes were determined by common methods. Results and discussion. At the background of the Leu-Ile-Lys-Met tetrapeptide, free radical kidney damage was weakened, which was manifested in the complete absence of morphological signs of nephrolithiasis, decrease of thiobarbituric acid-reactive product concentration and overall prooxidant activity as compared to the control group; glutathione peroxidase activity, on the contrary, increased by 1.5 times

Scanning Electron Microscopy Study of Drilling Cuttings in Tomsk Oblast Sites

The research is focused on determining mineralogical composition of drilling cuttings by scanning electron microscope as well as imaging the sample surface of high resolution that allows studying the structural characteristics of the site. In addition, a number of other techniques permit obtaining information on chemical composition of sample in near-surface layers. The study in drilling cuttings by means of scanning microscopy has revealed the presence of titanium, iron, zirconium oxides, iron sulphide, barium sulphate. The former is a mineral that concentrates rare-earth elements, presumably monocyte, as well as uranium silicate, etc. The results obtained confirm the data of previous X-ray structural analysis, i.e. the study samples consist of alumosilicate matrix. Apart from silicon and aluminium oxides, the matrix includes such elements as Na, K, Mg. Such a composition corresponds to rock-forming minerals: quartz, albite, microcline, clinochlore, muscovite, anorthoclase

Применение викасола как перспективного средства фармакологической коррекции экспериментального нефролитиаза

The aim of the investigation was studying vicasole’s effect on experimental oxalate nephrolithiasis.Experimental nephrolithiasis was being modeled by using of 1% ethylenglycole’s solution as a drink for rats during 6 weeks. First group was control. In the second group since the third week was being administrated vicasole in dose 500 mkg/kg. Was being detected parameters of kidney’s function, markers enzymes activity and free oxygen’s radicals activity, was carried out morphological researches.It was concluded that vicasole’s therapy reduce experimental oxalate nephrolithiasisПроведено исследование влияния викасола на течение экспериментального оксалатного нефролитиаза.Экспериментальный нефролитиаз моделировали у двух групп крыс путем потребления в течение 6 нед 1%-го раствора этиленгликоля в виде питья. Первая группа являлась контрольной. Во второй группе начиная с 3-й нед ежедневно вводили подкожно викасол в дозе 500 мкг/кг массы тела. Определяли показатели экскреторной функции почек, измеряли активность маркерных ферментов и процесса свободнорадикального окисления, а также проводили морфометрическое исследование почечных срезов.Установлено, что викасол существенно облегчает течение экспериментального нефролитиаза

Опыт применения натрия пирофосфата при оксалатном нефролитиазе в эксперименте

The aim of the investigation was studying sodium pyrophosphate’s effect on experimental oxalate nephrolithiasis.Experimental nephrolithiasis modeling by using of 1% ethylenglycole’s solution as a drink for rats during 6 weeks. First group was control. In the second group since the third week was being administrated sodium pyrophosphate in dose 2 g/kg. Was detecting parameters of kidney’s function, markers enzymes activity, was carried out morphological researches.It was concluded that sodium pyrophosphate’s therapy reduce experimental oxalate nephrolithiasis.Изучено влияние натрия пирофосфата на течение экспериментального оксалатного нефролитиаза.Экспериментальный нефролитиаз моделировался у двух групп крыс путем потребления в течение 6 нед 1%-го раствора этиленгликоля в виде питья. Первая группа являлась контрольной. Во второй группе начиная с 3-й нед ежедневно вводился внутрь натрия пирофосфат в дозе 2 г/кг массы тела. Осуществлялось определение показателей экскреторной функции почек, измерение активности маркерных ферментов, а также морфометрическое исследование почечных срезов.Установлено, что натрия пирофосфат существенно облегчает течение экспериментального нефролитиаза

The Role of Phosphatidylethanolamine-N-methyltransferase (PEMT) Gene rs12449964 Polymorphism in the Development of Acute Pancreatitis and its Complications

Background. Acute pancreatitis is considered to be an important issue in modern medicine. The phosphatidylethanolamine-N-methyltransferase enzyme plays a significant role in the regulation of lipid metabolism by catalyzing the process of methylation of phosphatidylethanolamine to phosphatidylcholine. These lipids are key components of mitochondrial and cell membranes, providing their fluid and plastic properties and participating in the transport of fats, fatty acids and cholesterol. Along with its function in the synthesis of phosphatidylcholine, the methylation of phosphatidylethanolamine promotes the turnover of S-adenosylmethionine for the synthesis of cysteine and glutathione through transulphurisation. PEMT is a gene encoding the phosphatidylethanolamine-N-methyltransferase enzyme.Aim. To determine the role of PEMT C/T rs12449964 polymorphism in the risk of developing acute pancreatitis and its complications among Russian residents in Central Russia.Materials and methods. Whole blood samples were collected from 502 unrelated patients with acute non-biliary pancreatitis (97 women and 405 men) of Russian nationality who had been admitted to the surgical departments of the city of Kursk from 2015 to 2018, as well as from 513 unrelated individuals of Russian nationality without gastrointestinal diseases (101 women and 412 men). The average age of patients and healthy individuals was 48.9 ± 13.1 and 47.89 ± 12.1 years, respectively. Genomic DNA was isolated by a standard phenol-chloroform extraction method. Genotyping of rs12449964 polymorphism was performed using real-time PCR by allelic discrimination using a CFX96 Bio-Rad Laboratories amplifier (USA) with TaqMan probes and commercial TaqMan SNP Genotyping Assays reagents purchased from Applied Biosystems (USA).Results. The study has shown that the frequency of the C allele and the C/C PEMT C/T rs12449964 genotype was higher in the group of patients with acute pancreatitis, while the C/T genotype was predominant in the control group. C/T — T/T genotypes demonstrated a protective effect on the development of infected pancreatic necrosis, purulent necrotic peripancreatitis and severe acute pancreatitis.Conclusions. The disruption of phosphatidylethanolamine methylation processes increases the sensitivity of cells to oxidative stress, which can lead to the development of acute pancreatitis