Physico-Chemical and Bacteriological Characteristics of Noyyal River and Ground Water Quality of Perur, India

The study was conducted to findout the physico-chemical and bacteriological characteristics of Noyyal River and ground water quality of Perur, India. The river water and ground water (bore well and open well) samples were collected in and around Perur and analyzed for various physico-chemical and bacteriological parameters. The study indicated that the mean value of studied parameters, except phosphate, biological oxygen demand (BOD), chemical oxygen demand (COD) of the river water and ground water samples were within the permissible limit. The parameters include total suspended solids (TSS-132.2 mgl-1), BOD (9.55 mgl-1), COD (19.28 mgl-1), phosphate (PO42--1.58 mgl-1) and total nitrogen (TN-2.04 mgl-1) of the river water sample were found higher when compared to the ground water samples. The bacteriological study showed that the total heterotrphic bacteria (THB -124.4 x 103 CFU/ml), Total Coliforms (TC-2600 MPN/10ml), Faecal Coliforms (FC-1750 MPN/10ml), Faecal Streptococci (FS-120 MPN/10ml) and FC/FS ratio (14.6) in the river water samples were found to be greater than the standard WHO limits. The generic distribution in the samples revealed that the presence of Escherichia coli , Staphylococcus, Enterobacter, Streptococci, Bacillus and Micrococcus were predominant in river water samples. The river water was found to be unfit for human consumption due to the exceeding level of Coliforms. The quality of ground water around Noyyal River was found to be safe and utilizable for cultivation purposes. If the quality management is properly maintained, the river water could be utilized for a wide range of application viz, irrigation, drinking etc. @ JASE

Comparative modelling and molecular docking of nitrate reductase from Bacillus weihenstephanensis (DS45)

Nitrate reductase catalyses the oxidation of NAD(P)H and the reduction of nitrate to nitrite. NR serves as a central point for the integration of metabolic pathways by governing the flux of reduced nitrogen through several regulatory mechanisms in plants, algae and fungi. Bacteria express nitrate reductases that convert nitrate to nitrite, but mammals lack these specific enzymes. The microbial nitrate reductase reduces toxic compounds to nontoxic compounds with the help of NAD(P)H. In the present study, our results revealed that Bacillus weihenstephanensis expresses a nitrate reductase enzyme, which was made to generate the 3D structure of the enzyme. Six different modelling servers, namely Phyre2, RaptorX, M4T Server, HHpred, SWISS MODEL and Mod Web, were used for comparative modelling of the structure. The model was validated with standard parameters (PROCHECK and Verify 3D). This study will be useful in the functional characterization of the nitrate reductase enzyme and its docking with nitrate molecules, as well as for use with autodocking

Physico-Chemical and Bacteriological Characteristics of Noyyal River and Ground Water Quality of Perur, India

The study was conducted to findout the physico-chemical and bacteriological characteristics of Noyyal River and ground water quality of Perur, India. The river water and ground water (bore well and open well) samples were collected in and around Perur and analyzed for various physico-chemical and bacteriological parameters. The study indicated that the mean value of studied parameters, except phosphate, biological oxygen demand (BOD), chemical oxygen demand (COD) of the river water and ground water samples were within the permissible limit. The parameters include total suspended solids (TSS-132.2 mgl-1), BOD (9.55 mgl-1), COD (19.28 mgl-1), phosphate (PO42--1.58 mgl-1) and total nitrogen (TN-2.04 mgl-1) of the river water sample were found higher when compared to the ground water samples. The bacteriological study showed that the total heterotrphic bacteria (THB -124.4 x 103 CFU/ml), Total Coliforms (TC-2600 MPN/10ml), Faecal Coliforms (FC-1750 MPN/10ml), Faecal Streptococci (FS-120 MPN/10ml) and FC/FS ratio (14.6) in the river water samples were found to be greater than the standard WHO limits. The generic distribution in the samples revealed that the presence of Escherichia coli , Staphylococcus, Enterobacter, Streptococci, Bacillus and Micrococcus were predominant in river water samples. The river water was found to be unfit for human consumption due to the exceeding level of Coliforms. The quality of ground water around Noyyal River was found to be safe and utilizable for cultivation purposes. If the quality management is properly maintained, the river water could be utilized for a wide range of application viz, irrigation, drinking etc. @ JASE