Adenohibernoma: A Rare Breast Tumor

WOS: 000304699400014PubMed: 21933783Adenohibernoma of the breast is a very rare tumor composed of brown fat tissue and intermingled glandular tissue. There are only 2 case reports in literature. This study reports the third case of adenohibernoma of the breast, which is not accompanied by breast cancer differing from the previous cases. And, to the best of the authors' knowledge, this is also the first case of adenohibernoma of the breast that has demonstrated adipophilin expression immunohistochemically

A Case of Two Synchronous Cutaneous Collision Tumors

Cutaneous collision tumors are known as two independent tumors which are close anatomically and separated from one another by well boundaries. We, herein report a 83-year-old female patient with two cutaneous collision tumors in two different localizations at the same time. First cutaneous collision tumor located on left ala nasi was squamous cell carcinoma and basal cell carcinoma and second one located on the right commisure was composed of malignant melanoma (Clark Level IV) and basal cell carcinoma. However, the presence of collision tumors is not uncommon and is often reported in the literature, to the best of our knowledge, it is the first case which shows the association of two synchronous cutaneous collision tumor in the same individual

Epithelial expression of extracellular matrix metalloproteinase inducer/CD147 and matrix metalloproteinase-2 in neoplasms and precursor lesions derived from cutaneous squamous cells: An immunohistochemical study

WOS: 000326996100003PubMed: 23948694Extracellular matrix metalloproteinase inducer (CD147) is a transmembrane glycoprotein involved in the regulation of matrix metalloproteinases (MMPs). The study investigated CD147 and MMP-2 expression in epidermis of cutaneous squamous lesions. CD147 and MMP-2 expressions were evaluated immunohistochemically in 44 specimens: 18 actinic keratoses (AK), 6 squamous cell carcinomas in situ (SCCIS), 13 squamous cell carcinomas (SCC; peritumoral and invasive portions assessed), and 7 normal skins. Patterns of expression were assessed, with MMP-2 in nuclei (MMP-2n) and cytoplasm (MMP-2c) evaluated separately. The expression of each marker was quantified using a calculated immunohistochemical/histologic score (H-score). Correlations were analyzed for the marker H-scores in each study group. Associations between H-scores and histopathologic parameters were also evaluated. CD147 H-score was the highest in SCC (invasive islands), followed by AK, SCCIS, and control specimens, respectively. MMP-2n and MMP-2c H-scores were the highest in AK, followed by SCCIS, SCC, and control specimens, respectively. MMP-2c and MMP-2n H-scores were significantly higher in peritumoral epidermis than in invasive islands of SCC. MMP-2c and CD147 H-scores were positively correlated in the peritumoral SCCs. CD147 H-score was positively correlated with tumor differentiation in SCC. The findings suggest that overexpression of CD147 plays a role in the development of SCC. (C) 2013 Elsevier GmbH. All rights reserved.Kirikkale University Scientific Research CommitteeKirikkale UniversityThe project was supported by Kirikkale University Scientific Research Committee

Neuroprotective effects of racemic ketamine and (S)-ketamine on spinal cord injury in rat

WOS: 000305422000026PubMed: 22436574Background: The aim of this study was to investigate and to compare the potential neuroprotective effects of racemic ketamine, (S)-ketamine and methylprednisolone after an experimental spinal cord injury model in rats. Methods: Fifty-nine Wistar albino rats were divided into three main groups as acute stage (A), subacute stage (SA) and sham groups and then acute and subacute stage groups were divided into four groups regarding the used drug as control (CONT), racemic ketamine (RK), (S)-ketamine (SK) and methylprednisolone (MP) groups. A dorsal laminectomy was performed; and spinal cord injury was induced by using a temporary aneurysm clip. Four hours later from the clip compression, except those of the sham and control groups, the drugs (60 mg/kg racemic ketamine, 60 mg/kg (S)-ketamine or 30 mg/kg methylprednisolone) were administered intraperitoneally. At 72th h and 7th days of the study, the spinal cords of rats were removed from T8 level to the conus medullaris level. The specimens were and evaluated histopathologically, tissue lipid peroxidation (LPO) and myeloperoxidation (MPO) levels were measured and biochemically. Results: The histopathological results were similar both in the acute and in the subacute stage groups. There was a statistically significant difference among all groups regarding the tissue LPO levels (p < 0.001). There was a statistically significant difference between the CONT-A group and the MP-A, RK-A and SK-A groups (p = 0.004, p < 0.001 and p = 0.007, respectively) in acute stage and between the CONT-SA group and SK-SA group (p = 0.002) in subacute stage. There was a statistically significant difference among all groups regarding the tissue MPO levels (p = 0.001). The median MPO levels were similar among acute stage groups (p = 0.057), but there was a statistical difference among subacute stage groups (p = 0.046). Conclusion: (S)-ketamine is more effective than methylprednisolone and racemic ketamine to reduce the LPO levels in subacute stage of spinal cord injury in rats. And, it is as effective as methylprednisolone in preventing secondary spinal cord injury histopathologically. (c) 2012 Elsevier Ltd. All rights reserved

Effects of low-dose methotrexate in spinal cord injury in rats

WOS: 000322097200002PubMed: 23884668BACKGROUND This study was designed to evaluate the possible protective effects of low-dose methotrexate in the spinal cord injury (SCI) in rats. METHODS Thirty-seven Wistar albino rats were used in the present study. Except for the animals of the Sham group, all animals were divided into two main groups, which were used in acute and subacute stage investigations. Then, thoracal laminectomy was performed, and except for the Sham group, SCI was induced using a temporary aneurysm clip. After clip compression, the experimental material (methotrexate or methylprednisolone) was administered intraperitoneally, except in the Sham and Control groups. Then, the spinal cords were removed to evaluate the SCI histopathologically and biochemically at the scheduled date. RESULTS Neither experimental material was shown to reduce the histopathological grade in either stage of SCI. Low-dose methotrexate was shown to decrease lipid peroxidation levels only in the subacute stage of SCI. However, methylprednisolone and low-dose methotrexate could not decrease or block myeloperoxidase enzyme activation in either stage of SCI. CONCLUSION Low-dose methotrexate was effective in reducing the lipid peroxidation levels in the subacute stage of SCI, although histopathological evaluation results and myeloperoxidase levels of all groups did not support this finding at either stage

Evaluation of protective effects of the alpha lipoic acid after spinal cord injury: An animal study

EVLIYAOGLU, CETIN AYHAN/0000-0002-1774-3084; GAZYAGCI, SERKAL/0000-0002-0043-6942WOS: 000281587300016PubMed: 20637463Purpose: Many of the hypotheses have been suggested to explain the mechanism of the secondary effects of traumatic spinal cord injury (TSCI) as follows: ischaemia/reperfusion disability theory, free radicals theory, exitotoxicity theory, immunological destruction theory, apoptosis theory. Recently, free radicals, lipid peroxidation reactions and apoptosis theories have been much more accepted than others. In this study, possible protective effects of the alpha lipoic acid were evaluated in the traumatic spinal cord of rats. Methods: Using a well characterised weight drop technique, spinal cord contusions were formed to 48 Wistar albino rats at thoracal 8-10 level. After alpha lipoic acid and methylprednisolone were administered intraperitoneally, the spinal cord tissues were harvested for histopathological and biochemical studies. Results: Histopathological examination results showed that neither methylprednisolone nor alpha lipoic acid can play an act to decrease or block the neural tissue destruction and necrosis in hyperacute and subacute stage of the TSCI in rats. Biochemical study results showed that alpha lipoic acid was much more decreased the lipid peroxidation levels than methylprednisolone in subacute stage. However, none of the agents was changed the myeloperoxidation level in subacute stage. Interpretation: Alpha lipoic acid and methylprednisolone administrations did not alter the onset or degree of necrosis at the zone of the TSCI in rats. On the other hand, alpha lipoic acid is more effective than methylprednisolone treatment for the prevention of lipid peroxidation after spinal cord injury. (C) 2010 Elsevier Ltd. All rights reserved

Effects of low-dose methotrexate in spinal cord injury in rats

WOS: 000322097200002PubMed: 23884668BACKGROUND This study was designed to evaluate the possible protective effects of low-dose methotrexate in the spinal cord injury (SCI) in rats. METHODS Thirty-seven Wistar albino rats were used in the present study. Except for the animals of the Sham group, all animals were divided into two main groups, which were used in acute and subacute stage investigations. Then, thoracal laminectomy was performed, and except for the Sham group, SCI was induced using a temporary aneurysm clip. After clip compression, the experimental material (methotrexate or methylprednisolone) was administered intraperitoneally, except in the Sham and Control groups. Then, the spinal cords were removed to evaluate the SCI histopathologically and biochemically at the scheduled date. RESULTS Neither experimental material was shown to reduce the histopathological grade in either stage of SCI. Low-dose methotrexate was shown to decrease lipid peroxidation levels only in the subacute stage of SCI. However, methylprednisolone and low-dose methotrexate could not decrease or block myeloperoxidase enzyme activation in either stage of SCI. CONCLUSION Low-dose methotrexate was effective in reducing the lipid peroxidation levels in the subacute stage of SCI, although histopathological evaluation results and myeloperoxidase levels of all groups did not support this finding at either stage

Effect of P/E-selectin blockage on antisperm antibody development and histopathological alterations in experimental orchitis

KISA, Ucler/0000-0002-8131-6810; Soyer, Tutku/0000-0003-1505-6042WOS: 000325332200029PubMed: 24094975Aim: This study aimed to evaluate the effect of P/E-selectin blockage on antisperm antibody (ASA) development and histopathological alterations in experimental orchitis. Materials and Methods: Thirty-six Wistar albino-type male rats weighing 100-150 g were included in the study. Rats were allocated into six groups (n = 6) including control (CG), sham (SG), orchitis (OG), antimicrobial treatment (AG), P/E-selectin blockage (PESG), and both antimicrobial and P/E-selectin treatment (TG) groups. In CG, serum samples were taken from the tail vein prior to the procedure and followed by extraction of both testes. In SG, 1 ml of saline solution was injected in testicular parenchyma. OG was obtained by injecting 0.1 ml 106 cfu/ml Escherichia coli (0: 6 strain) and 1 ml saline solution into the right testes. AG received ciprofloxacin (50 mg/kg/day) twice a day through gastrogavage 24 hours after generating orchitis. In PESG, P/E-selectin antibody (100 mu g) was administered intravenously via the tail vein 24 hours after the induction of orchitis. Finally, both ciprofloxacin and P/E-selectin antibody were administered in TG 24 hours after the induction of orchitis for 14 days. At the end of treatment, 1 ml of serum sample was obtained to evaluate the ASA, P-selectin and E-selectin levels. In order to evaluate spermatogenesis (Johnsen score) and testicular injury (Cosentino score), both testes were extracted at the end of the 14th day. Results: In orchitis-induced groups (OG, ATG, PSEG, TG), ASA levels were significantly increased at the 14th day when compared to SG (p 0.05). In OG and AG, P-selectin levels were decreased at the 14th day when compared to levels observed on 0 day (p 0.05). There was no significant difference regarding E-selectin when compared to CG (p > 0.05). No significant differences regarding E-selectin levels were detected on the 0th and 14th days between AG and CG (p > 0.05). When the Cosentino and Johnsen scores were compared among groups, TG and PSEG has decreased scores of Cosentino than OG on the right testicle (p 0.05). There was no difference with regard to the right and left testicular injury in TG. In P/E-blocked groups, decreased histopathological alterations were observed in the contralateral testis. Conclusion: P/E-selectin blockage may reduce ASA production after orchitis when combined with antimicrobial treatment. P/E-selectin blockage not only has a protective effect on blood-testis barrier but also decreases the histopathological alterations in both the affected and contralateral testis. Histopathological parameters of spermatogenesis may also be prevented by P/E-selectin blockage in experimental orchitis. (C) 2013 Elsevier Inc. All rights reserved.Kirikkale University Scientific Research CouncilKirikkale UniversityThis study was supported by Kirikkale University Scientific Research Council and presented at the National Congress of Turkish Pediatric Surgeons, Istanbul, Turkey, 2011