EVALUATION OF IN VIVO EFFECTS OF VISCUM ALBUM L. AND ITS CONSTITUENT O-COUMARIC ACID ON BILE ACID SYNTHESIZING CYPS

This research was designed to determine about the possible potency of medicinal plants Viscum album L. and its major polyphenolic ingredient, o-coumaric acid on rat liver cholesterol and bile acid metabolizing enzymes. Bioactive compounds of V. album extracts were identified using LC-MS technique. The water extracts of V. album (10 mg/kg) and o-coumaric acid (30 mg/kg) were injected intraperitoneally for 9 days. Then, in vivo effects of this plant and its major phenolic ingredient (o-coumaric acid) on rat liver cholesterol and bile acid metabolizing cytochromes P450s were analyzed by determining protein and mRNA expression levels using western blotting and qRT-PCR techniques, respectively. The results showed that V. album caused a 0.9 fold decrease in protein expression of CYP7A1 and CYP7B1, but 1.4 and 1.3 fold increases in protein expression of CYP27A1 and CYP8B I, respectively. V. album caused 9, 1.3 and 2.4 fold decrease in mRNA expression of CYP7A1, CYP27A1, CYP7B1, respectively, however, 4.2 fold increase in mRNA expression of CYP8B1. Injection of o-coumaric acid to rats resulted 0.8 fold decrease in protein expression of CYP7A1, CYP27A1, CYP8B1 and CYP7B1. However, o-coumaric acid caused 1.8, 1.3, 2.3 and 2.3 fold increase in mRNA expression of CYP7A1, CYP27A1, CYP8B 1 and CYP7B1, respectively. According to results, even though V. album and o-coumaric acid caused fold change in protein and mRNA expressions of bile acid metabolizing CYPs, serum cholesterol levels remained unchanged. Thus, the idea can be taken about the role of this plant and its major polyphenolic ingredient in drug discovery and development

The current status of ethnopharmacobotanical knowledge in Camlidere (Ankara, Turkey)

The main objective of this study is to identify and record the folk medicines used in Camlidere (Ankara) as well as to contribute to the preservation of this precious lore. For this purpose field trips were organized to Camlidere. A total of 79 taxa belonging to 66 genera and 33 families were recorded for the treatment of various disorders. The most represented families were Asteraceae, Lamiaceae, and Rosaceae, respectively. Respiratory tract diseases were the principal reasons for using folk medicines. Eight new folk medicines were included in the Turkish ethnobotanical repository with this study. Different ethnobotanical usages such as fuel and food were also noted. Our data obtained from the research area showed some uses of plants that were newly introduced to the folk medicinal literature of Turkey. Moreover, ``Use value{''}, ``Informant consensus factor{''}, and ``Cultural importance index{''} were also calculated to evaluate the data statistically

Investigations on the anti-ulcerogenic activity of Sideritis caesarea H. Duman, Aytac & Baser

WOS: 000536887800018PubMed: 32360801Ethnopharmacological relevance: Aerial parts of Sideritis caesarea H. Duman, Aytac & Baser are used for complaints such as stomach-aches, and intestinal spasms as traditional medicine in Kayseri, Turkey. Aim of study: To investigate the anti-ulcerogenic activity by using bioassay guided fractionation technique (BAGF) and to identify the compound(s) that are responsible for anti-ulcerogenic activity through ethanol-induced anti-ulcerogenic activity model in vivo. Materials and methods: Liquid-liquid partition and then different chromatographic techniques were utilized for the BAGF of the ethanol (80%) extract of the aerial parts of Sideritis caesarea. Ethanol-induced gastric ulcer method on rats was employed for the determination of the anti-ulcerogenic activity, and the ulcer index was also calculated for anti-ulcerogenic activity detection. Results: the ethanol (80%) extract of S. caesarea showed statistically potent anti-ulcerogenic activity (95.9% ulcer inhibition, p 2)]- 6 ''-O-acetyl-beta-D-glucopyranoside and isoscutellarein-7-O-[6'''-O-acetyl-beta-D-allopyranosyl-(1 -> 2)]-6 ''-O-acetyl-beta-D-glucopyranoside} were isolated from this fraction together with a sesquiterpene glycoside [(2E,6E)-2,6,10-trimethyl-2,6,11-dodecatriene-1,10-diol-1-O-beta-D-glucopyranoside] and two additional flavonoids {4'-O-methylhypolaetin-7-O-[6'''-O-acetyl-beta-D-allopyranosyl-(1 -> 2)]-beta-D-glucopyranoside and isoscutellarein- 7-O-[6'''-O-acetyl-beta-D-allopyranosyl-(1 -> 2)]-beta-D-glucopyranoside}. Conclusions: Traditional use of S. caesarea in the treatment of stomach-aches was supported by this study and four flavonoids were isolated by using BAGF method and two of them were determined to have significant antiulcerogenic activity. Additionally, (2E,6E)-2,6,10-trimethyl-2,6,11-dodecatriene-1,10-diol-1-O-beta-D-glucopyranoside was obtained from a Sideritis genus for the first time.Scientific and Technological Research Council of Turkey-TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TBAG-112S581]This study was financially supported by "The Scientific and Technological Research Council of Turkey-TUBITAK" (project number: TBAG-112S581)

Folk Medicine In Duzce Province (Turkey)

The present study was conducted to collect, record, and document local knowledge of medicinal practices in Duzce, a northwestern Anatolian province. To the best of our knowledge, no comprehensive ethnobotanical study has been reported from this province. Information was acquired through semistructured interviews and personal conversations using a questionnaire and numerous guided field trips with local knowledgeable people. For quantitative analyses and comparisons, recorded data such as informant consensus factor (F-IC) and use value (UV) were calculated, respectively. As a result of extensive field studies, 122 taxa were determined as folk medicines; 76 of were wild and 46 were cultivated. The identified medicinal plants were mainly from the family Rosaceae, followed by Compositae, Apiaceae, Lamiaceae, and Solanaceae, respectively. Among the preparations used, liquid forms such as infusions (30.2%) or decoctions (16.4%) represented the most favored ways to administer medicinal plants. Dermatological disorders had the highest F-IC score with a value of 0.75 followed by skeletomuscular (F-IC = 0.7466), gastrointestinal (F-IC = 0.6666), immunological (F-IC = 0.6615), and respiratory (F-IC = 0.6292) system disorders, among others. The most prominent medicinal plants were Urtica dioica (UV = 0.4352), Plantago major (UV = 0.3056), Rubus ulmifolius (UV = 0.2279), and Sambucus ebulus (UV = 0.2279). According to the present study, the number of people who recognize and use the wild plants of Duzce, and those of the rest of Anatolia, is steadily decreasing. The ethnobotanical knowledge cannot be passed to the next generation in its entirety if it is not properly recorded. In addition to this gradual loss of knowledge, modern information pollution and contamination via the popular media highlight the urgent need to record this precious knowledge before it is lost.WoSScopu