In vitro Evolution and Engineering for Improved Ribozyme Polymerase Production

According to the "RNA World Hypothesis" the first self replicating molecule formed from the prebiotic chemicals is the ribozyme. A model compartmentalized self replicating system for ribozyme polymerases is designed and used to improve activity, fidelity and generality of the enzyme

Effect of stem cell and vitamin E for the reduction of liver fibrosis

Liver disease is seventh leading cause of death worldwide. In the past, liver transplantation was thought to be the only treatment for the last stage liver disease but currently stem cells therapy is an alternative method for the treatment of liver disease. So mesenchymal stem cells (MSCs) transplantation is one of the best tool for treatment of liver disease. The aim of the current study was to investigate the combined effect of vitamin E (Vit E) and MSCs on liver fibrosis. Liver damage was induced in male albino mice intraperitoneally with carbon tetrachloride (CCl4) twice a week for six weeks. Mice bone marrow derived MSC was cultured in vitro and then transplanted to CCl4 injured mice through their tail vain injection. Two weeks after MSCs transplantation, all group of mice were examined. The morphological result showed that the combined therapy of Vit E (orally) and MSCs transplantation have significant therapeutic effect on CCl4 injured mice as compared to Vit E and MSCs individually. Biochemical data also showed that serum ALT and bilirubin level were found to be significantly decreased by the combined treatment of Vit E and MSCs as compared to those mice which received MSCs and Vit E separately. MSCs and Vit E treated mice combined showed a significant decrease in liver weight, closely to normal. Reverse transcriptase PCR result also confirmed a significant anti fibrotic effect of Vit E combined with MSCs transplanted mice on liver fibrosis as showed by down-regulating apoptotic marker (Bax) expression and increasing the expression of antiapoptotic marker (Bcl-xl). Therefore Vit E along with MSCs have strong therapeutic potential on liver fibrosis in CCl4 injured mice

Enhancing Knowledge of Family Caregivers and Quality of Life of Patients with Ischemic Stroke

Objectives:  A Quasi-Experimental study was conducted to determine the impact of Attend-trial-based interventions on the knowledge of the caregivers and the quality of life of Ischemic stroke survivors. Material and Methods:  The study was conducted in the acute care hospital located in the rural area, Khyber Pukhtoonkha. A sample of 115 patients and informal caregivers participated in a multifaceted intervention including educational sessions, training and demonstration, and post-training discussion via WhatsApp. A pre-post evaluation of the quality of life, stroke knowledge, functioning, and activities of daily living was completed using validated tools. All information was collected using predesign pro forma and questionnaire. Results:  The interventions significantly improved all four outcomes in caregivers and patients. The mean score for the WHOQOL-BREF pre-intervention was 2.91 ± 1.10 which was increased to 4.18 ± 0.86. The pre-knowledge scores were 34.00 which increased to 39.95. There was a statistically significant change between pre and post knowledge scores [t (114) = -3.394, p = 0.001]. Conclusion:  The study contributed towards the usefulness of multifaceted education intervention to enhance the rehabilitation efforts of patients and their caregivers in community settings

Improvement of Serum Biochemical Parameters and Hematological Indices Through α-Tocopherol Administration in Dietary Oxidized Olive Oil Induced Toxicity in Rats

Dietary oxidized olive oil, alone or in combination with different doses of α-tocopherol, were given to Swiss albino rats for 30 days; in order to determine its role in oxidative stress and fatty liver, induced by the oxidized olive oils. Serum biochemical parameters and hematological indices of blood were analyzed. The liver was analyzed for histopathological changes, lipid peroxidation, and polar triacylglycerols composition. Results revealed that there was a significant decline in the serum total cholesterol, triglycerides, LDL, glucose and ALT; while a significant increase occurred in the serum HDL levels through the supplementation of α-tocopherol in male and female rats. Hematological parameters were almost in the normal reference range in the groups that were fed α-tocopherol, alone or in combination with oxidized oil, while being significantly altered by the oxidized olive oil. There were acute hepatitis and necrosis in the liver with no fatty changes after feeding with oxidized olive oil, along with varying doses of α-tocopherol. Higher amounts of polar compounds were present in female rats (15.2–93.1 μg/g) compared to male rats (12.2–82.3%) that correspond to the supplementation of α-tocopherol in combination with oxidized oil. Lipid oxidation in liver was minimized by tocopherol, while an increase occurred in the accumulation of oxidized lipids in the liver. These findings revealed that tocopherol is beneficial against the oxidized oil induced biochemical and hematological changes and lipid peroxidation but causes fatty accumulation in the liver. Therefore, the role of tocopherol in patients with fatty liver disease may be considered, as tocopherol may increase the chance of survival

Prevalence of HBV and HBV vaccination coverage in health care workers of tertiary hospitals of Peshawar, Pakistan

<p>Abstract</p> <p>Background</p> <p>Hepatitis B Virus (HBV) may progress to serious consequences and increase dramatically beyond endemic dimensions that transmits to or from health care workers (HCWs) during routine investigation in their work places. Basic aim of this study was to canvass the safety of HCWs and determine the prevalence of HBV and its possible association with occupational and non-occupational risk factors. Hepatitis B vaccination coverage level and main barriers to vaccination were also taken in account.</p> <p>Results</p> <p>A total of 824 health care workers were randomly selected from three major hospitals of Peshawar, Khyber Pakhtunkhwa. Blood samples were analyzed in Department of Zoology, Kohat University of Science and Technology Kohat, and relevant information was obtained by means of preset questionnaire. HCWs in the studied hospitals showed 2.18% prevalence of positive HBV. Nurses and technicians were more prone to occupational exposure and to HBV infection. There was significant difference between vaccinated and non-vaccinated HCWs as well as between the doctors and all other categories. Barriers to complete vaccination, in spite of good knowledge of subjects in this regard were work pressure (39.8%), negligence (38.8%) un-affordability (20.9%), and unavailability (0.5%).</p> <p>Conclusions</p> <p>Special preventive measures (universal precaution and vaccination), which are fundamental way to protect HCW against HBV infection should be adopted.</p

Comparing the Effectiveness of Different Reinforcement Stimuli in a Robotic Therapy for Children With ASD

Recent research has shown reliability in robotic therapies for improvement in core impairments of autism. To improve the efficiency of communication using robots, this study evaluates the effectiveness of three different stimuli in a robotic intervention for children with autism spectrum disorder. Three different reinforcement stimuli presented in least-to-most (LTM) order introduced in this therapy using NAO robot are: visual (color variation), auditory and motion cues. The therapy was tested on 12 ASD children, 4 out of 12 children fall under mild category whereas 8 fall under the minimal category of autism. The experimentation was conducted for 2 months. Total 8 experiments were conducted with 1 trial per week. Total 12 cues were given per trial, 4 cues corresponding to each category. In total 96 cues were given per subject, 32 cues from each category. The results indicate a general trend for linking a particular autism category with the most effective stimulus for that category. It can be concluded that visual cue (color variation) is the most effective reinforcement stimulus for children with minimal autism as 8 out of 8 i.e., 100% were more responsive to visual cues whereas for children with mild autism category, 3 out of 4 i.e., 75% are more receptive towards the motion stimulus. The parameters used for assessment were joint attention and the time eye contact is maintained. Single factor ANOVA was used for the statistical analysis of results with alpha is 0.05 and p-value 0.0342, F value is 3.7456 and F critical value is 3.2834. The test was performed on 96 ( $8\times 12$ ) trails in total, therefore ensuring the significance and reliability of our results

Exploring organizational management of extrovert school leadership

The study aimed at determining the personality traits of extroversion and introversion of school leadership and evaluating their organizational management in Federal Government Educational Institutions (FGEIs). A quantitative research design along with a positivist paradigm was followed. The total population consisted of 68 school principals and 592 teachers of FGEIs in the Gujranwala, Fazaia, and Lahore Regions from which 54 principals and 381 teachers were selected using a random sampling technique. Eysenck Personality Inventory (EPI) was deployed to collect information about personality traits and data regarding organizational management, the researchers used a self-developed questionnaire. Pearson correlation r and t-tests were deployed for inferential stats. The results showed that extrovert school leaders perform their duties well as they hold a clear concept of the vision and mission of the organization, set up an accepted procedure to plan short-term and long-term goals, share responsibilities with all stakeholders following their skills, and ensure the smooth functioning of the school with the help of all stakeholders. In comparison, introverted school principals show less interest in the organizational management of their schools. Moreover, organizational management of school leadership has a positive correlation with the personality trait of extroversion and a significant difference can be seen in organizational management of an extrovert and an introvert school principal. It was recommended that the performance indicators and standards of school principals should be effectively communicated to school leadership. Introvert school leadership should be provided with the necessary psychological training to reduce this shortcoming of personality

Breeding performance of sustainable fish Ctenopharyngodon idella through single intramuscular injection of Ovaprim-C at Bahawalpur, Pakistan

Effect of intramuscular injection of Ovaprim–C on the number of eggs/kg,  fertilization rate and hatching percentage were studied at a private Fish Hatchery at Bahawalpur, Pakistan, during April to June 2008, on Ctenopharyngodon idella (Grass carp). Studied fish specimens were spawned successfully following a single dose of injection of Ovaprim-C (LH-RH analogue) with 0.6 ml kg-1 for female and 0.2 ml kg-1 for male brooders. Ova and milt were stripped simultaneously and mixture was stirred for 15 to 30 s during which fertilization occurred. Hatching occurred within 18 to 30 h after fertilization. The experiment was conducted in circular spawning tank with 2 m diameter. It was observed that body weight has positive influence on absolute fecundity (r = 0.967), while relative fecundity remained constant with increasing body weight. If it is impossible to determine the absolute and relative fecundity then these parameters can be determined from the body weight.Key words: Induced spawning, Ovaprim-C, fecundity, Ctenopharyngodon idella

A migration aware scheduling technique for real-time aperiodic tasks over multiprocessor systems

Multi-processor systems consist of more than one processor and are mostly used for computationally intensive applications. Real-time systems are those systems that require completing execution of tasks within a pre-defined deadline. Traditionally, multiprocessor systems are given attention in periodic models, where tasks are executed at regular intervals of time. Gradually, as maturity in a multiprocessor design had increased; their usage has become very common for real-time systems to execute both periodic and aperiodic tasks. As the priority of an aperiodic task is usually but not essentially greater than the priority of a periodic task, they must be completed within the deadline. There is a lot of research works on multiprocessor systems with scheduling of periodic tasks, but the task scheduling is relatively remained unexplored for a mixed workload of both periodic and aperiodic tasks. Moreover, higher energy consumption is another main issue in multiprocessor systems. Although it could be reduced by using the energy-aware scheduling technique, the response time of aperiodic tasks still increases. In the literature, various techniques were suggested to decrease the energy consumption of these systems. However, the study on reducing the response time of aperiodic tasks is limited. In this paper, we propose a scheduling technique that: 1) executes aperiodic tasks at full speed and migrates periodic tasks to other processors if their deadline is earlier than aperiodic tasks-reduces the response time and 2) executes aperiodic tasks with lower speed by identifying appropriate processor speed without affecting the response time-reduces energy consumption. Through simulations, we demonstrate the efficiency of the proposed algorithm and we show that our algorithm also outperforms the well-known total bandwidth server algorithm