ミトコンドリアADP/ATP輸送体の新規阻害剤エポキシシクロヘキセンジオン類の作用機構研究

京都大学新制・課程博士博士(農学)甲第23247号農博第2454号新制||農||1084(附属図書館)学位論文||R3||N5337京都大学大学院農学研究科応用生命科学専攻(主査)教授 三芳 秀人, 教授 宮川 恒, 教授 森 直樹学位規則第4条第1項該当Doctor of Agricultural ScienceKyoto UniversityDGA

Cytotoxicity of prostaglandin analog eye drops preserved with benzalkonium chloride in multiple corneoconjunctival cell lines

Masahiko Ayaki1, Atsuo Iwasawa21Department of Ophthalmology, Saitama National Hospital, Wako, 2Department of Clinical Pathology, Showa University Fujigaoka Hospital, Yokohama, JapanPurpose: This study evaluated the cytotoxicity of five prostaglandin analog ophthalmic ­solutions on four ocular surface cell lines, ie, Chang (human conjunctiva), SIRC (rabbit cornea), RC-1 (rabbit cornea), and BCE C/D-1b (bovine cornea).Methods: Cell viability was measured by neutral red and MTT assays in cells treated for 10, 30, or 60 minutes with various doses of prostaglandins (undiluted, and 2- and 10-fold dilutions). The number of cell lines with viability ≥50% in the presence of selected dilution of the drug (CVS50) was used for comparison. In addition, 24 cell viability comparisons (four cell lines, two assays, and three exposure times) were made between latanoprost (Xalatan®) and each other solution at each dose. A comparison between the newly introduced tafluprost (Tapros®) with 0.01% benzalkonium chloride was also made.Results: The order of cell viability determined by CVS50 was Travatan Z® (travoprost with the SofZia system)> Tapros ≥ Travatan® (travoprost) = Xalatan > Rescula® (unoproston). This was consistent with the results of direct comparisons between Xalatan and the other drugs. There was no clear difference in cell viability between Tapros and benzalkonium chloride.Conclusions: Use of two assays, multiple cell lines, and various dilutions and exposure times provided a unique evaluation of cytotoxicity among ophthalmic solutions. CVS50 was useful for comparison of the cell viability of the solutions.Keywords: glaucoma, cornea, cell viability scor

Towards highly accurate calculations of parity violation in chiral molecules: relativistic coupled-cluster theory including QED-effects

Parity-violating energies $E_{PV}$ of the $H_2X_2$ X = O, S, Se, Te, Po) molecules are reported, calculated as analytical expectation values at the relativistic coupled-cluster singles-and-doubles (CCSD) level using property-optimized basis sets. Radiative corrections to the $E_{PV}$ was investigated using effective QED-potentials and found to reach a maximal value of 2.38% for $H_2Po_2$ . However, this result depends on the choice of effective self-energy potential and may indicate limitations to their domain of validity.Comment: 15 page

THE INFLUENCE OF AUTOMOTIVE INDUSTRIAL WORK PRACTICE COMPETENCIES ON THE READINESS FOR THE AUTOMOTIVE WORLD OF AUTOMOTIVE WORK IN MECHANICAL ENGINEERING STUDENTS, PALANGKA RAYA UNIVERSITY

Industrial Work Practice Experience can provide skills and additional knowledge for students to be ready to work. This is because students have seen and are used to the real world of work. When students carry out Industrial Work Practices, students are required to be serious in doing a job so that they have experience that can be useful in the future. If the student is not serious, the student will not be used to the actual world of work environment and the student's skills will be lacking, so there will be no Job Readiness after the student graduates later. This research is a research using quantitative research methods. This research was conducted at the Mechanical Engineering Education Study Program FKIP UPR which was carried out for one Even Semester of the 2021/2022 Academic Year. The population in this study were all students of the Mechanical Engineering Education Study Program, FKIP, University of Palangka Raya who had taken industrial work practices from class 2015 to 2019, totaling 148 people. Then the sample will be taken to be a total of 28 samples of Mechanical Engineering Education students at the University of Palangka Raya.Based on the results of calculating the correlation coefficient as follows: r 0.996 100% based on these results there is a positive correlation of 0.996, so the conclusion is that there is a positive relationship and the value of the correlation coefficient between student automotive practice skills and automotive laboratory management is 0.996. The reliability value of the statement items in the questionnaire for each variable being studied is greater than 0.70. This result indicates that the statement items in the questionnaire are reliable for measuring the variables. Based on the results of the calculation of variable X with a significant level of α 0.05 and dk k-1 21, the value of X²table is 32.671, it turns out that X²count X²table or 3472 32.671 for variable X is normally distributed. And based on the results of the calculation of the Y variable with a significant level of α 0.05 and dk k-1 21, the X²table value is 32.671, it turns out that X²count X²table or 3301 32.671, for the Y variable is normally distributed. The results of the hypothesis test show that variable X (student automotive practice ability) has a significant effect on variable Y (Readiness to Face the World of Automotive Work) and means that H0 is rejected and Ha is accepted

SELF PERCEPTION ABOUT THE COMPETENCE OF MECHANICAL ENGINEERING EDUCATION STUDENTS AS PROSPECTIVE TEACHERS

This study aims to describe the competencies possessed by mechanical engineering education students based on self-perception. These competencies are pedagogic competence, personality competence, sosial competence and professional competence. The results showed that students of the Mechanical Engineering Education Study Program (PTM), Palangka Raya University (UPR) had quite good pedagogical competence, good personality competence, good sosial competence and fairly good professional competence

Enhancement of parity-violating energy difference of CHFClBr, CHFClI, and CHFBrI by breaking the cancellation among valence orbital contributions

The enhancement of the parity-violating energy difference (PVED) by electronic excitation is studied for H$_2X_2$ ($X =$ O, S, Se, Te), CHFClBr, CHFClI, and CHFBrI. To clarify the enhancement mechanism, the dihedral angle dependence of the PVED of H$_2X_2$ in excited states is studied. If the contribution from the highest occupied molecular orbital (HOMO) to the PVED in the ground state is larger than the sum of those from all occupied orbitals, the PVED in the first excited state has a much larger value compared to the ground state due to cancellation breaking among valence orbital contributions. This enhancement is named cancellation breaking enhancement. The PVED enhancement is also studied for CHFClBr, CHFClI, and CHFBrI in excited states, and the cancellation breaking enhancement is confirmed. When the PVED contribution from the HOMO is larger than any other contribution, the cancellation breaking enhancement hypothesis provides the estimate of PVED in the first excited state from the HOMO contribution.Comment: Some typos are fixe

USER’S OPINIONS ON ANDROID-BASED LEARNING

The purpose of this research is to find out the user's response to e-module based on android products to facilitate learning process. Likert scale with four variations of the answer was the scale used in this study. Research data were analyzed by quantitative descriptive. The result of the research showed that e-module based on android products have met the eligibility criteria with the category "very good" so it is worth to use. The data of the research showed that User’s response to e-module in this case teachers and students obtained “very good” category with the feasibility percentage of each are 84.38% and 81.67%

Toxicity of antiglaucoma drugs with and without benzalkonium chloride to cultured human corneal endothelial cells

Masahiko Ayaki1, Atsuo Iwasawa2, Yoichi Inoue31Department of Ophthalmology, Saitama National Hospital, Wako, Japan; 2Life Particle Interaction Engineering Creation, New Industry Creation Hatchery Center, Tohoku University, Sendai, Japan; 3Department of Ophthalmology, Olympia Eye Hospital, Tokyo, JapanPurpose: The toxicity of antiglaucoma medications to ocular surface cells has been evaluated extensively; however, the toxicity to corneal endothelial cells (CECs) remains elusive. Our aim is to evaluate the toxicity of antiglaucoma medications to CECs using an in vitro toxicity assay.Methods: Primary cultures of human (H) CECs derived from eye bank specimens were established. Following exposure of HCECs to test solutions for 10, 30, or 60 minutes, or 48 hours, we measured cell viability using a WST-1 assay. Test solutions were diluted in culture media and included 0.5% Timoptol®, preservative-free 0.5% timolol maleate, 1% Trusopt®, preservative-free 1% dorzolamide, Travatan®, Travatan Z®, Xalatan®, and benzalkonium chloride (BAK). To assess cell viability, the value of the test culture well after treatment was expressed as a percentage of that of the control well. Toxicity of each solution was compared using the cell viability score (CVS).Results: After exposure to 10-fold dilutions of test solutions for 48 hours, HCEC viabilities were 48.5% for 0.5% Timoptol, 80.9% for preservative-free 0.5% timolol maleate, 47.0% for 1% Trusopt, 71.7% for preservative-free 1% dorzolamide, 55.5% for Travatan, 88.5% for Travatan Z, and 52.5% for Xalatan. Exposure to test solutions diluted 100-fold or more resulted in HCEC viabilities > 80%, with the exception of preservative-free 1% dorzolamide, which resulted in a viability of 72.0% at a dilution of 100-fold. Based on CVS, the order of cell viability was Travatan Z ≥ preservative-free timolol maleate = preservative-free dorzolamide > 0.5% Timoptol = 1% Trusopt > Travatan ≥ Xalatan. Assessment of the combined effect of drug and BAK revealed that latanoprost reduced the toxicity of BAK.Conclusion: Antiglaucoma eye drops produced HCEC toxicity that appeared to depend on the presence of BAK. Because dilution of the antiglaucoma solutions resulted in markedly lower HCEC toxicity, HCEC damage due to antiglaucoma medication may occur only in rare cases. The CVS was useful for comparison of the toxicity of the drugs.Keywords: cell viability score, eye drop, preservative

分子中のCP対称性破れに関する相対論的量子化学に基づく理論的研究：増幅機構の解明およびQED効果を考慮した精密計算手法の開発

首都大学東

A Case of Nevoid Acanthosis Nigricans Successfully Treated with Topical Ketoconazole Plus Urea

Nevoid acanthosis nigricans (AN) is a rare form of benign AN that can be mostly found as a solitary lesion distributed along Blaschko’s lines (1). It is not associated with any known syndrome, endocrinopathy, drugs, or internal malignancy. Treatments include retinoid, calcipotriol, and laser treatments (2). Herein we report a case of nevoid AN successfully treated with topical ketoconazole plus ure