Onset and duration of cycloplegic action of 1% cyclopentolate – 1% tropicamide combination

Purpose: To study the time course (onset, time and duration of maximal cycloplegia, and the full duration) of cycloplegic action of 1% Cyclopentolate – 1% Tropicamide.Methods: Seventy-seven students, aged 15-24 years were purposively sampled from the University of Cape Coast and Cape Coast Technical Institute. Subjective near addition (ADD) determination and pupil diameter measurement before and after a drop of the test agent (1% Cyclopentolate – 1% Tropicamide combination in the right eye) and the control (1% Cyclopentolate in the left eye) were performed. Measurements of subjective near ADD and pupil diameter were made after the initial reading at 5 minutes interval for the first hour and every 30 minutes for the next 7 hours for each participant. Time of onset, time of peak cycloplegia, duration of peak cycloplegia and duration of total cycloplegic effect was indirectly determined.Results: 1% Cyclopentolate – 1% Tropicamide combination had rapid onset of cycloplegia (5-10 minutes), shorter time of maximal cycloplegia (55 versus 90 minutes), and recovery (7 versus ≥ 8hours) in the majority (79.2%) of subjects.Conclusion: Cyclopentolate –Tropicamide combination was comparable to Cyclopentolate in depth of cycloplegia produced, and clinically superior to 1% Cyclopentolate in rapidity of cycloplegic onset, time of maximal cycloplegia and recovery from cycloplegia.Keywords: Cycloplegia, time course, accommodation, recovery tim

Efficiency of Helminth eggs removal in dewatered faecal sludge by co-composting

This study aimed at investigating helminth eggs removal efficiency in dewatered faecal sludge (FS) co-composted with organic solid waste as related to the turning frequency. A mixture of fresh public toilet sludge and septage mixed in a ratio of 1:2 was dewatered on a drying bed. Biosolids with initial load of 25-83 helminth eggs/g TS was mixed with solid waste as bulking material in a volume ratio of 1:2 for co-composting. Two replicate sets of compost heaps were mounted in parallel and turned at different frequencies: i) once each 3 days, ii) once each 10 days, during the active composting period, to study the influence of the operational conditions on the compost hygienic quality. The helminth egg removal efficiency was not different for the two turning frequencies. In both setups helminth eggs were reduced from 25-83 /g TS to a, < 1-3 viable eggs/g TS level, allowing a safety reuse in agriculture

Evaluation of the IgG antibody response to SARS CoV-2 infection and performance of a lateral flow immunoassay: cross-sectional and longitudinal analysis over 11 months

OBJECTIVE: To evaluate the dynamics and longevity of the humoral immune response to SARS-CoV-2 infection and assess the performance of professional use of the UK-RTC AbC-19 Rapid Test lateral flow immunoassay (LFIA) for the target condition of SARS-CoV-2 spike protein IgG antibodies. DESIGN: Nationwide serological study. SETTING: Northern Ireland, UK, May 2020–February 2021. PARTICIPANTS: Plasma samples were collected from a diverse cohort of individuals from the general public (n=279), Northern Ireland healthcare workers (n=195), pre-pandemic blood donations and research studies (n=223) and through a convalescent plasma programme (n=183). Plasma donors (n=101) were followed with sequential samples over 11 months post-symptom onset. MAIN OUTCOME MEASURES: SARS-CoV-2 antibody levels in plasma samples using Roche Elecsys Anti-SARS-CoV-2 IgG/IgA/IgM, Abbott SARS-CoV-2 IgG and EuroImmun IgG SARS-CoV-2 ELISA immunoassays over time. UK-RTC AbC-19 LFIA sensitivity and specificity, estimated using a three-reference standard system to establish a characterised panel of 330 positive and 488 negative SARS-CoV-2 IgG samples. RESULTS: We detected persistence of SARS-CoV-2 IgG antibodies for up to 10 months post-infection, across a minimum of two laboratory immunoassays. On the known positive cohort, the UK-RTC AbC-19 LFIA showed a sensitivity of 97.58% (95.28% to 98.95%) and on known negatives, showed specificity of 99.59% (98.53 % to 99.95%). CONCLUSIONS: Through comprehensive analysis of a cohort of pre-pandemic and pandemic individuals, we show detectable levels of IgG antibodies, lasting over 46 weeks when assessed by EuroImmun ELISA, providing insight to antibody levels at later time points post-infection. We show good laboratory validation performance metrics for the AbC-19 rapid test for SARS-CoV-2 spike protein IgG antibody detection in a laboratory-based setting

Onset and duration of cycloplegic action of 1% cyclopentolate \u2013 1% tropicamide combination.

Purpose: To study the time course (onset, time and duration of maximal cycloplegia, and the full duration) of cycloplegic action of 1% Cyclopentolate \u2013 1% Tropicamide. Methods: Seventy-seven students, aged 15-24 years were purposively sampled from the University of Cape Coast and Cape Coast Technical Institute. Subjective near addition (ADD) determination and pupil diameter measurement before and after a drop of the test agent (1% Cyclopentolate \u2013 1% Tropicamide combination in the right eye) and the control (1% Cyclopentolate in the left eye) were performed. Measurements of subjective near ADD and pupil diameter were made after the initial reading at 5 minutes interval for the first hour and every 30 minutes for the next 7 hours for each participant. Time of onset, time of peak cycloplegia, duration of peak cycloplegia and duration of total cycloplegic effect was indirectly determined. Results: 1% Cyclopentolate \u2013 1% Tropicamide combination had rapid onset of cycloplegia (5-10 minutes), shorter time of maximal cycloplegia (55 versus 90 minutes), and recovery (7 versus 65 8hours) in the majority (79.2%) of subjects. Conclusion: Cyclopentolate \u2013Tropicamide combination was comparable to Cyclopentolate in depth of cycloplegia produced, and clinically superior to 1% Cyclopentolate in rapidity of cycloplegic onset, time of maximal cycloplegia and recovery from cycloplegia

Visual function among commercial vehicle drivers in the central region of Ghana

Aim: To determine the relationship between some visual functions: colour vision defects, abnormal stereopsis, visual acuity and the occurrence of road traffic accident (RTAs) among commercial vehicle drivers in the central region of Ghana, and to assess their knowledge of these anomalies. Method: A descriptive cross-sectional study employing a multi-stage random sampling approach was conducted in the major commercial towns within the central region of Ghana. Participants were taken through a comprehensive eye examination after the administration of a structured questionnaire. Results: 520 male commercial vehicle drivers were enrolled for this study with a mean age of 39.23 years ±10.96 years and mean visual acuity of 0.02 ± 0.08 logMAR. Protans were more likely to be involved in RTAs (χ2 = 6.194, p = 0.034). However, there was no statistically significant association between abnormal stereopsis (OR = 0.89 95% CI: 0.44–1.80, p = 0.56), poor vision due to refractive error (χ2 = 3.090, p = 0.388) and the occurrence of RTAs. While 86.9% were aware of abnormal stereopsis, only 45% were aware of colour vision defects. There was a statistically significant association between stereopsis anomaly and colour vision defect (r = 0.371, p < 0.005). Conclusion: The study found an association between protanopia and RTAs but none between stereopsis anomalies, refractive errors and the occurrence of RTAs. Drivers were less knowledgeable on colour vision defects as compared to stereopsis anomalies

Assessment of indices of conjunctival microvascular function in patients with and without obstructive coronary artery disease

Background: Atherosclerotic heart disease often remains asymptomatic until presentation with a major adverse cardiovascular event. Primary preventive therapies improve outcomes, but conventional screening often misattributes risk. Vascular imaging can be utilised to detect atherosclerosis, but often involves ionising radiation. The conjunctiva is a readily accessible vascular network allowing non-invasive hemodynamic evaluation. Aim: To compare conjunctival microcirculatory function in patients with and without obstructive coronary artery disease. Methods: We compared the conjunctival microcirculation of myocardial infarction patients (MI-cohort) to controls with no obstructive coronary artery disease (NO-CAD cohort). Conjunctival imaging was performed using a smartphone and slit-lamp biomicroscope combination. Microvascular indices of axial (V a) and cross-sectional (V cs) velocity; blood flow rate (Q); and wall shear rate (WSR) were compared in all conjunctival vessels between 5 and 45 μm in diameter. Results: A total of 127 patients were recruited (66 MI vs 61 NO-CAD) and 3602 conjunctival vessels analysed (2414 MI vs 1188 NO-CAD). Mean V a, V cs and Q were significantly lower in the MI vs NO-CAD cohort (V a 0.50 ± 0.17 mm/s vs 0.55 ± 0.15 mm/s, p &lt; 0.001; V cs 0.35 ± 0.12 mm/s vs 0.38 ± 0.10 mm/s, p &lt; 0.001; Q 154 ± 116 pl/s vs 198 ± 130 pl/s, p &lt; 0.001). To correct for differences in mean vessel diameter, WSR was compared in 10–36 μm vessels (3268/3602 vessels) and was lower in the MI-cohort (134 ± 64 s −1 vs 140 ± 63 s −1, p = 0.002). Conclusions: Conjunctival microcirculatory alterations can be observed in patients with obstructive coronary artery disease. The conjunctival microvasculature merits further evaluation in cardiovascular risk screening.</p