Resistin and adenylyl cyclase-associated protein 1 (CAP1) regulate the expression of genes related to insulin resistance in BNL CL.2 mouse liver cells

© 2019 The Author(s) Resistin is an adipokine produced in white adipose tissue that is thought to modulate insulin sensitivity in peripheral tissues (such as liver, skeletal muscle or adipose tissue). Human and murine resistin molecules share only about 60% sequence homology. [1] Contrary to humans, in which resistin is secreted mostly by macrophages, Park and Ahima 2013 resistin in rodents is produced primarily by the mature adipocytes of the white adipose tissue. Although resistin can bind to toll-like receptor 4 (TLF4) activating proinflammatory responses in human and rodents, [3–8] the inflammatory actions of resistin in human monocytes were found to be mediated by resistin binding to adenylyl cyclase-associated protein 1 (CAP1). [9] In this study, we aimed to investigate the in vitro effects of resistin on the expression of various genes related to insulin resistance in mouse liver cells. Using BNL CL.2 cells, we investigated the effect of resistin in untransfected or CAP1 siRNA-transfected cells on the expression of 84 key genes involved in insulin resistance

In vitro effects of resistin on epithelial to mesenchymal transition (EMT) in MCF-7 and MDA-MB-231 breast cancer cells - qRT-PCR and Westen blot analyses data.

Resistin is an adipokine produced by the white adipocytes and adipose-derived macrophages, which mediates inflammation and insulin resistance Huang et al., 1997 and Renehan et al., 2008 Feb. Here, we provide data on the effect of resistin on epithelial to mesenchymal transition (EMT) in breast cancer cells in vitro. As model systems, we used human MCF-7 (low-metastatic) and MDA-MB-231 (high-metastatic) breast cancer cell lines. To optimize experimental conditions, we treated the cells with various concentrations of resistin (12.5, 25 and 50 ng/ml) for different time intervals (6 and 24 hours), and measured SOCS3 mRNA expression by using qRT-PCR analysis. Further, we used qRT-PCR and Western blot analyses to measure the expression of various epithelial (E-cadherin, claudin-1) and mesenchymal (SNAIL, SLUG, ZEB1, TWIST1, fibronectin, and vimentin) markers after resistin treatment. This data article is part of a study Avtanski et al., 2019 May, where detailed interpretation and discussion can be found

Cytokine secretion in breast cancer cells – MILLIPLEX assay data

© 2019 The Author(s) Metastatic breast cancer is the most advanced stage of breast cancer and the leading cause of breast cancer mortality. Although understanding of the cancer progression and metastasis process has improved, the bi-directional communication between the tumor cell and the tumor microenvironment is still not well understood. Breast cancer cells are highly secretory, and their secretory activity is modulated by a variety of inflammatory stimuli present in the tumor microenvironment. Here, we characterized the cytokine expression in human breast cancer cells (MDA-MB-231, MCF-7, T-47D, and BT-474) in vitro using 41 cytokine MILLIPLEX assay. Further, we compared cytokine expression in breast cancer cells to those in non-tumorigenic human breast epithelial MCF-10A cells

Glycosylated hemoglobin, but not advanced glycation end products, predicts severity of coronary artery disease in patients with or without diabetes.

Background:The association between coronary artery disease (CAD) and diabetes mellitus (DM) is strong but the physiologic mechanisms responsible for this association remain unclear. Patients with DM exhibit high circulating levels of glycated proteins and lipoproteins called advanced glycation end products (AGEs) which have been implicated in the development of oxidative damage to vascular endothelium. We examined the relationships between the presence and extent of CAD and AGEs in patients undergoing elective coronary artery catheterization in an urban teaching hospital. Methods:Patients with possible CAD (n = 364) were recruited prior to elective cardiac catheterization (52% male, 48% diabetic). Regression and correlation analyses were used to examine the relationship between serum AGE concentrations, soluble AGE receptor (sRAGE) concentration, HbA1c, LDL and the presence of obstructive CAD along with the burden of CAD measured by SYNTAX and SYNTAX II scores. Results:AGE and sRAGE levels did not significantly correlate with any of the studied coronary artery disease parameters. HbA1c showed positive correlation with both SYNTAX and SYNTAX II scores in patients with and without diabetes. Conclusion:In this cross-sectional study of patients with possible CAD, serum AGEs and sRAGE concentrations did not correlate with SYNTAX or SYNTAX II scores regardless of diabetic status. HbA1C correlated positively with the SYNTAX and SYNTAX II scores in both diabetic and non-diabetic populations

A concept for control of indoor-operated autonomous wheelchair

The present chapter introduces a navigation algorithm for a wheelchair in semistructured home environment. The user sets the end position and orientation of the wheelchair and the controller autonomously steers the wheelchair from the current position to the goal. The algorithm includes a procedure for automatic creation of an initial map of the wheelchair surroundings that is used afterwards for composition of collision-free path from the starting point to the target. Calculation of the present wheelchair position is based on the information of the TV images from ceiling mounted cameras that detect the wheelchair in all steps of its movement toward the goal. For prevention from navigation failures in situations when wheelchair markers cannot be clearly detected by the cameras, the information about the angular rotation of the driving wheels is used for calculation of the current position. In order to set the movement task, the user refers to a wheelchair-mounted monitor where the images from all rooms are represented. After selection of the TV image from the room where the target is located, one puts the end wheelchair position and orientation by pointing them directly on the image. The information, collected by onboard-mounted range sensors during the wheelchair operation, is used for obstacle avoidance and map update. The map can be additionally corrected and modified by the user. A special simulator ROSI (RObotic SImulator) is designed for computer testing of the proposed algorithm. Finally, some design principles of the simulator and results from the initial evaluation of the algorithm with the simulation are remarked

Evaluation of the in vitro effects of irisin and leptin on human ovarian granulosa cells – PCR array data (female infertility panel)

Human ovarian granulosa cells were purified from follicular fluid samples obtained from patients undergoing in vitro fertilization procedure by using Percoll PLUS reagent (GE Healthcare, Cat. # 17-5445-02). Cells were grown in DMEM/F12 (50:50) medium (Corning, Cat. # 10-092-CM) supplemented with 10% FBS (VWR, Cat. # 89510-186) and antibiotic/antimycotic mixture (MP Biomedicals, Cat. # 1674049). For experiment, 0.3 x 10(6) cells were seeded in 6-well plates and the following day the cell culture medium was replaced with serum-free medium supplemented with 500 ng/ml of irisin (Enzo, Cat. # ADI-908-307-0010) or 50 ng/ml of leptin (BioVision, Cat. # 4366-02) for 24 hours. After completing the experiment, cells were washed two times with PBS (Corning, Cat. # 46-013-CM) and total RNA was extracted using TRIzol reagent (Ambion, 15596018). RNA was quantified using NanoDrop One spectrophotometer (Thermo Fisher Scientific) and samples were normalized to 1 mg/ml concentration, then converted to cDNA using qScript cDNA SuperMix (Quantabio, Cat. # 95048). PCR array was performed using RT2 Profiler PCR Array – Human Female Infertility (Qiagen, Cat. # 330231, GeneGlobe ID PAHS-164Z) and QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific) following manufacturer’s protocol. PCR array data were calculated using GeneGlobe Data Analysis Center (https://geneglobe.qiagen.com/us/analyze).THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

Expression of diabetes-related genes in rodent β-cell line (RIN-m) treated with monocarbonyl curcumin analogues (C66 and B2BrBC) in presence or absence of streptozotocin – PCR array data

Curcumin monocarbonyl analogues C66* and B2BrBC** were synthesized at the Institute of Chemistry, Ss. Cyril and Methodius University in Skopje, Macedonia by substituting benzaldehyde with cyclohexanone in a base catalyzed aldol reaction. RIN-m rat β-cells (ATCC, Cat. # CRL-2057) were grown in RPMI-1640 medium (ATCC, Cat. # 30-2001) supplemented with 10% FBS (VWR, Cat. # 89510-186) and antibiotic/antimycotic mixture (MP Biomedicals, Cat. # 1674049) in incubator with standard conditions (5% CO2 and temperature of 37 °C). For experiments, 0.3 x 10(6) cells were seeded in 6-well plates with tissue culture growth media. The following day the media was replaced with serum-free media supplemented with 1.5 mM of streptozotocin (Sigma-Aldrich Cat. # 85882), 50 µM of C66, or B2BrBC dissolved in DMSO (VWR, Cat. # N182), and combination of streptozotocin with C66 or B2BrBC, for 72 hours. Total RNA was extracted using TRIzol reagent (Ambion, Cat. # 15596018). The quantification of RNA was made using NanoDrop One spectrophotometer (Thermo Fisher Scientific) after which the samples were normalized to 1 µg total RNA and converted to cDNA using qScript cDNA SuperMix (Quantabio, Cat. # 95048). PCR array was performed using QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific) and RT2 Profiler PCR Array – Rat Diabetes (Qiagen, Cat. # 330231, GeneGlobe ID PARN-023ZA). The Ct values were obtained using QuantStudio Design & Analysis Software.* (2E,6E)-2,6-bis[(2-trifluoromethyl)benzylidene]cyclohexanone, C22H16F6O** (2E,6E)-2,6-bis(2-bromobenzylidene)cyclohexanone), C20H16Br2OTHIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

A novel system for wheelchair stability assessment : design and initial results

Wheelchairs should provide safe and reliable functioning in various road conditions and situations, ease of propulsion, and high maneuverability. Often, wheelchairs need further modification and installation of additional equipment (ventilators, oxygen cylinders, etc) which affect their stability. Wheelchair stability is also dependent on a user's body characteristics that can result in a shifting of the centre of mass e.g. limb amputations, and obesity, etc. Adaptation of the wheelchair requires additional assessment and wheelchair tuning by highly skilled rehabilitation engineers. In this paper, we discuss the design and initial testing of a novel wheelchair stability assessment system. The developed WheelSense system consists of a force platform that senses the weight distribution of the wheelchair, the centre of the contact points, and the distances between contact points of the wheels. The measurement platform is linked via WiFi connection to a portable tablet computer where the platform's sensor signals are processed and the wheelchair stability parameters are calculated. An intuitive touchscreen GUI is used for visualization of the stability results and navigation through separate measurement modes. The mechanical platform was designed to be foldable and light-weighted and thus, to be easily transportable which gives additional advantages when the system is used outside of a clinical engineering department. The initial design is being evaluated through four prototype systems installed for clinical testing in 3 large hospitals in the UK. The initial results indicate that the developed measurement system possesses high accuracy and ease of operatio