Evaluation of some systemic inflammatory biomarkers in canine malignant mammary tumors

The aim of this study is to investigate whether neutrophil-to-lymphocyte ratio (NLR), lymphocyte-to-monocyte ratio (LMR), platelet-to-lymphocyte ratio (PLR), systemic immune-inflammation index (SII), albumin-to-globulin ratio (AGR), and prognostic nutritional index (PNI) parameters could be used as biomarkers for canine malignant mammary tumors (MMTs), and the changes in these parameters according to different tumor (T), lymph node (N), and metastasis (M) stages (TNM I-II-III, TNM IV, TNM V) and the number of affected mammary glands (single, multiple). Thirty-seven with MMT and 20 healthy dogs were used in this study. Complete blood count and biochemistry analysis were performed in all dogs. Tumor material is removed by tru-cut and sent to the pathology laboratory for diagnosis. NLR, PLR, and SII values increased, and LMR and PNI values decreased in dogs with MMT. Median NLR values increased and median LMR and PNI values decreased as the TNM stage progressed. In dogs with a single MMT, median NLR, and PLR values were found to be lower than in dogs with multiple MMTs, and median LMR, SII, AGR, and PNI values were higher. The present results indicated that NLR, LMR, PLR, SII, and PNI parameters could be used as biomarkers for canine MMT. Also, NLR, LMR, PLR, SII, PNI, and AGR parameters may be valuable biomarkers that reveal the degree of systemic immune response according to different TNM stages and the number of affected mammary glands

The mid-term results of treatment for tibial pilon fractures

BACKGROUN

A COMPARISON OF ORTHOPAEDIC TRAUMA CASES OPERATED ON DURING THE COVID-19 PANDEMIC WITH DIFFERENT PERIODS: A SINGLE CENTRE STUDY

Introduction: To compare orthopaedic trauma cases treated surgically in our clinic during the COVID-19 pandemic in Turkey with surgically-treated trauma cases in previous periods, and to discuss these in the light of literature. Materials and Methods: Patient data of 3 different periods were compared. Group 1 included cases in the time period 11 March - 30 May, when the hospital was operating as a pandemic hospital for the COVID-19 pandemic in Turkey. Group 2 included cases from the equivalent time period the year before the pandemic, thought to have similar patient characteristics, and Group 3 covered the time period immediately before 11 March when COVID-19 was known in the world but there had not been any cases diagnosed in Turkey. Operations were performed in our clinic because of orthopaedic trauma to 186 patients in Group 1, to 262 patients in Group 2, and to 261 patients in Group 3. Results: A decrease of 29% was observed in trauma cases during the pandemic. In Group 1, 62 of 186 patients were aged >65 years, and 43 of these (69%) had a hip fracture. In Group 2, 33 (58%) patients aged >65 years underwent surgery for hip fracture and in Group 3, 60 (75%) patients. The time from hospital admission to surgery was mean 3.76±3.55 days in Group 1, 3.18±3.08 days in Group 2, and 2.68±2.33 days in Group 3 (p=0.017). The number of cases of attempted suicides was 6, 3, 3, respectively in the three groups (p=0.184). 30-day mortality was determined in 5, 3, and 4 cases, respectively (p=0.460). Conclusions: A decrease in the number of trauma cases was observed during the pandemic. However, there was no such decrease in hip fractures in elderly patients despite the quarantine of those aged >65 years. The workforce of the clinic was divided up during the pandemic for the diagnosis and follow-up of COVID-19 cases and thus there was no reduction in the workload of orthopedists as trauma surgery continued at the rate of 71%. Therefore, it can be considered very important to apply strict regulations which will decrease the risk of the spread of infection, and implement a multidisciplinary work flow for the more rapid discharge of trauma patients

In vitro evaluation of Tc-99m- EDDA/tricine-HYNIC-Q-Litorin in gastrin-releasing peptide receptor positive tumor cell lines

WOS: 000318356800007PubMed ID: 23301856Bombesin and its derivatives exhibit a high affinity for gastrin-releasing peptide receptor (GRPr), which is over-expressed in a variety of human cancers (prostate, pancreatic, lung, etc.). The aim of this study was to investigate the in vitro potential of the hydrazinonicotinamide (HYNIC)-Q-Litorin. Tc-99m labeling was performed by using different co-ligands: tricine and ethylenediamine diacetic acid (EDDA). The radiochemical stability of radiolabeled peptide conjugates was checked at room temperature and in cysteine solution up to 24 h. The in vitro cell uptake of Tc-99m-EDDA-HYNIC-Q-Litorin and Tc-99m-tricine-HYNIC-Q-Litorin were evaluated on pancreatic tumor and control cell lines. Optimum specific activity and incubation time were determined for all the cell lines. The results showed that the cell uptake of the radiolabeled peptide conjugates in tumor cell lines were higher than in the control cell line. The findings of this study indicated the need for further development of in vivo study as a radiopharmaceutical for pancreatic tumor imaging.Scientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK); Scientific Project [108S200]; Ege University Science-Technology Application and Research CenterEge University [2009 BIL 025]The authors gratefully acknowledge the financial support received from the Scientific and Technological Research Council of Turkey, Scientific Project (108S200) and Ege University Science-Technology Application and Research Center (2009 BIL 025). The authors have declared no conflict of interest

UroVysion fluorescence in situ hybridization (UroVysion FISH) assay for detection of bladder cancer in voided urine of Turkish patients: a preliminary study

WOS: 000319175700009PubMed ID: 23788983Bladder cancer is the fourth most common cancer in men and the fifth most common cancer worldwide. UroVysion FISH has high sensitivity and specificity for urothelial carcinoma detection. We investigated the genetic marker detected by the UroVysion FISH technique in diagnosis of Turkish bladder cancer patients and compared these results with the urine cytology and cystoscopy. Urine specimens were analyzed using UroVysion FISH probes for abnormalities in centromeric chromosomes 3, 7, and 17 and locus-specific 9p21. Morning fresh voided urine samples were collected from each patient for FISH analysis. Cytology and histopathology analysis were performed by the pathology department. Twenty-seven bladder cancer patients (23 male and 4 female) with a history of bladder cancer who provided informed consent were included in this prospective study. The results showed that cancer was detected in 8 patients via FISH; 7 via cytology; 12 via cystoscopy. According to the pathology results, 15 were normal, 10 high-grade carcinoma and 2 low-grade carcinoma. Sensitivity of these methods with FISH, cytology, and cystoscopy was 29.6%, 25.9%, and 44.4%, respectively. In conclusion, all tests have different advantages and disadvantages. Also, larger studies will be needed to confirm these results. But, UroVysion FISH appeared to have good specificity for detecting bladder cancer in urine specimens and also it is important to correlate the FISH results with the cystoscopy and cytological findings

Evaluation of IL-32 levels in gingival tissue and serum of experimental periodontitis model

Aim: Interleukin (IL)-32, a recently discovered proinflammatory cytokine, is demonstrated in several infectious diseases. The goal of this study is to investigate the levels of IL-6, IL-10 and IL-32 in gingival tissue and serum of rats with experimental periodontitis. Material and Methods: Experimental periodontitis was induced by placing a silk ligature around the cervix of both sides of mandibular first molars in each male rat except for control group (Group 1). Thirty male Wistar albino rats were randomly divided into three groups of ten animals each as experimental periodontitis groups (Group 2, ligated for seven days; Group 3, ligated for fourteen days) and periodontally healthy control group (Group 1). At the end of experimental period, rats were sacrificed, and histomorphometric analyses were performed on the mandibles. IL-32, IL-10 and IL-6 levels were measured in gingival tissue and serum samples by ELISA. Results: Alveolar bone and attachment loss were statistically higher in all experimental groups than those in control group (P<0.001). It was found that the levels of IL-32 and IL-6 (P<0.01) and IL-10 (P<0.05) in gingival tissues were higher in Groups 2 and 3 than those in Group 1 except for IL-32 and IL-10 levels in Group 3. There was a positive correlation between levels of IL-32 and IL-6 in the serum and gingival tissues in all groups (P<0.05). Conclusion: The present results reveal that IL-32 values are locally increased in periodontitis. Proinflammatory cytokines properties, which are linked to periodontal tissue destruction, are also associated with IL-32