Further studies on the mode-of-action of the alpha hemolysin of Staphylococcus aureus role of the receptor in hemolysis.

Dept. of Biological Sciences. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis1984 .A877. Source: Masters Abstracts International, Volume: 40-07, page: . Thesis (M.Sc.)--University of Windsor (Canada), 1984

Structural And Chemical Characterization Of The S-layer Of Lampropedia Hyalina

The S-layer of Lampropedia hyalina is made of two separate layers. The inner, perforate layer, is composed of a 32kD polypeptide arranged in p6 symmetry with a lattice spacing of 14.6nm. The outer, punctate layer, is composed of tubular units connected by fine linking arms centred on p3 symmetry axes, to created a layer with p6 symmetry and a lattice constant of 25.6nm. Incubation of cell envelopes with 3M urea dissolved the punctuate layer and released three polypeptides of 60kD, 66kD and 240kD. These polypeptides reassembled to form the native punctate layer when dialysed against buffer containing 10mM CaCl{dollar}\sb2{dollar} or 10mM SrCl{dollar}\sb2{dollar}. The urea soluble polypeptides were fractionated by hydroxylapatite column chromatography. The isolated 60kD polypeptide formed ring-like structures, while the isolated 240kD polypeptide was visualized in negative stain as long, slightly curved threads. Fractions containing both the 60kD and 240kD polypeptides also contained complete assemblies of the punctate layer. This suggests that the central tubular units are composed of the 60kD polypeptide, while the linking arms consist of the 240kD polypeptide. One other fraction (66kD) may mediate attachment of the punctate layer to the underlying perforate layer thus forming the whole complex S-layer from at least four distinct polypeptides

Movement Of 1970 Year Class Striped Bass Between Virginia, New York And New England

Striped bass (Marone saxatilis) were tagged in Virginia beginning in 1968. The 1970 yearclass of striped bass was tagged both in Virginia and New York in 1972. Fish tagged in Virginia were returned from New York to Maine while fish tagged in New York were returned from the Maryland portion of the Chesapeake Bay and the Potomac River. These data indicated that fish migrate from rivers in which they were spawned at different ages and that fish that migrate as 2 year olds remain together as a group until they are 3+ years. Therefore, within the Chesapeake Bay area there are distinct river populations at least until these populations are 3+ years old.https://scholarworks.wm.edu/vimsbooks/1111/thumbnail.jp

The evolution of a coastal carbon store over the last millennium

This work was financially supported by the Natural Environment Research Council (grant number: NE/L501852/1), the EU FPV HOLSMEER project (EVK2-CT-2000-00060) and the EU FPVI Millennium project (contract number 017008), Biotechnology and Biological Sciences Research Council (grant number: BB/M026620/1) with additional support from the NERC Radiocarbon Facility (Allocation 1154.1005 and 2195.1019).Fjord sediments are recognized as hotspots for the burial and storage of organic carbon, yet little is known about the long-term drivers of significant terrestrial organic carbon (OC) transfers into these coastal carbon stores. The mid-latitude fjord catchments of Scotland have a long history of human occupation and environmental disturbance. We provide new evidence to show that increased anthropogenic disturbances over the last 500 years appear to have driven a step change in the magnitude of terrestrial OC transported to the coastal ocean. Increased pressures from mining, agriculture and forestry over the latter half of the last millennium have destabilized catchment soils and remobilized deep stores of aged OC from the catchment to the coastal ocean. Here we show that fjord sediments are capable of acting as highly responsive and effective terrestrial OC sinks, with OC accumulation rates increasing up to 20 % during the peak period of anthropogenic disturbance. The responsiveness and magnitude of the fjord OC sink represents a potentially significant time-evolving component of the global carbon cycle that is currently not recognized but has the potential to become increasingly important in the understanding of the role of these coastal carbon stores in our climate system.PostprintPeer reviewe

Associate Directors

$5.00 Up-to-date information about CPR’s research projects and other activities is available from our World Wide Web site at www-cpr.maxwell.syr.edu. All recent working papers and Policy Briefs can be read and/or printed from there a

Carrier-envelope phase stability of hollow-fibers used for high-energy, few-cycle pulse generation

We investigated the carrier-envelope phase (CEP) stability of a hollow-fiber setup used for high-energy, few-cycle pulse generation. Saturation of the output pulse energy is observed at 0.6 mJ for a 260 um inner-diameter, 1 m long fiber, statically filled with neon, with the pressure adjusted to achieve an output spectrum capable of supporting sub-4fs pulses. The maximum output pulse energy can be increased to 0.8mJ by using either differential pumping, or circularly polarized input pulses. We observe the onset of an ionization-induced CEP instability, which does not increase beyond an input pulse energy of 1.25 mJ due to losses in the fiber caused by ionization. There is no significant difference in the CEP stability with differential pumping compared to static-fill, demonstrating that gas flow in differentially pumped fibers does not degrade the CEP stabilization.Comment: 4 pages, 4 figure

Improved HMM Models for High Performance Speech Recognition

In this paper we report on the various techniques that we tmplemented in order to improve the basic speech recognition performance of the BYBLOS system. Some ot these methods are new, while others are not. We present methods that improved pertbrmance as well as those that did not. The methods include Linear Discrirninan

The impact of Hurricane Sandy on the shoreface and inner shelf of Fire Island, New York : large bedform migration but limited erosion

© The Author(s), 2015. This is the author's version of the work. It is posted here by permission of Elsevier for personal use, not for redistribution.. The definitive version was published in Continental Shelf Research 98 (2015): 13-25, doi:10.1016/j.csr.2015.03.001.We investigate the impact of superstorm Sandy on the lower shoreface and inner shelf offshore the barrier island system of Fire Island, NY using before-and-after surveys involving swath bathymetry, backscatter and CHIRP acoustic reflection data. As sea level rises over the long term, the shoreface and inner shelf are eroded as barrier islands migrate landward; large storms like Sandy are thought to be a primary driver of this largely evolutionary process. The “before” data were collected in 2011 by the U.S. Geological Survey as part of a long-term investigation of the Fire Island barrier system. The “after” data were collected in January, 2013, ~two months after the storm. Surprisingly, no widespread erosional event was observed. Rather, the primary impact of Sandy on the shoreface and inner shelf was to force migration of major bedforms (sand ridges and sorted bedforms) 10’s of meters WSW alongshore, decreasing in migration distance with increasing water depth. Although greater in rate, this migratory behavior is no different than observations made over the 15-year span prior to the 2011 survey. Stratigraphic observations of buried, offshore-thinning fluvial channels indicate that long-term erosion of older sediments is focused in water depths ranging from the base of the shoreface (~13-16 m) to ~21 m on the inner shelf, which is coincident with the range of depth over which sand ridges and sorted bedforms migrated in response to Sandy. We hypothesize that bedform migration regulates erosion over these water depths and controls the formation of a widely observed transgressive ravinement; focusing erosion of older material occurs at the base of the stoss (upcurrent) flank of the bedforms. Secondary storm impacts include the formation of ephemeral hummocky bedforms and the deposition of a mud event layer.This work was funded primarily by a rapid response grant from the Jackson School of Geosciences, The University of Texas/Austi