Protection of Glial Müller Cells by Dexamethasone in a Mouse Model of Surgically Induced Blood-Retinal Barrier Breakdown.

Purpose: Breakdown of the inner blood-retinal barrier (iBRB) occurs in many retinal disorders and may cause retinal edema often responsible for vision loss. Dexamethasone is used in clinical practice to restore iBRB. The aim of this study was to characterize the impact of a surgically induced iBRB breakdown on retinal homeostatic changes due to dystrophin Dp71, aquaporin-4 (AQP4), and Kir4.1 alterations in Müller glial cells (MGC) in a mouse model. The protective effect of dexamethasone was assessed in this model. Moreover, retinal explants were used to control MGC exposure to a hypoosmotic solution containing barium. Methods: Partial lens surgery was performed in C57BL6/J mice. Dystrophin Dp71, AQP4, and Kir4.1 expression was analyzed by quantitative RT-PCR, Western blot, and immunohistochemistry. Twenty-four hours after surgery, mice received a single intravitreal injection of dexamethasone or of vehicle. Results: After partial lens surgery, iBRB permeability increased while Dp71 and AQP4 were downregulated and Kir4.1 was delocalized. These effects were partially prevented by dexamethasone injection. In the retinal explant model, MGC were swollen and Dp71, AQP4, and Kir4.1 were downregulated after exposure to a hypoosmotic solution containing barium, but not in the presence of dexamethasone. Heat shock factor protein 1 (HSF1) was overexpressed in dexamethasone-treated retinas. Conclusions: Partial lens surgery induces iBRB breakdown and molecular changes in MGC, including a downregulation of Dp71 and AQP4 and the delocalization of Kir4.1. Dexamethasone seems to protect retina from these molecular changes by upregulating HSF1

Theoretical and experimental study of quasisteady-flow separation within the glottis during phonation : application to a modified two-mass model

Most flow models used in numerical simulation of voiced sound production rely, for the sake of simplicity, upon a certain number of assumptions. While most of these assumptions constitute reasonable first approximations, others appear more doubtful. In particular, it is implicitly assumed that the air flow through the glottal channel separates from the walls at a fixed point. Since this assumption appears quite unrealistic, and considering that the position of the separation point is an important parameter in phonation models, in this paper a revised fluid mechanical description of the air flow through the glottis is proposed, in which the separation point is allowed to move. This theoretical model, as well as the assumptions made, are validated using steady- and unsteady-flow measurements combined with flow visualizations. In order to evaluate the effective impact of the revised theory, we then present an application to a simple mechanical model of the vocal cords derived from the classical two-mass model. As expected, implementation of a moving separation point appears to be of great importance for the modeling of glottal signals. It is further shown that the numerical model coupled with a more realistic description of the vocal cord collision can lead to signals surprisingly close to those observed in real speech by inverse filtering

Theoretical and experimental study of quasisteady-flow separation within the glottis during phonation : application to a modified two-mass model

Most flow models used in numerical simulation of voiced sound production rely, for the sake of simplicity, upon a certain number of assumptions. While most of these assumptions constitute reasonable first approximations, others appear more doubtful. In particular, it is implicitly assumed that the air flow through the glottal channel separates from the walls at a fixed point. Since this assumption appears quite unrealistic, and considering that the position of the separation point is an important parameter in phonation models, in this paper a revised fluid mechanical description of the air flow through the glottis is proposed, in which the separation point is allowed to move. This theoretical model, as well as the assumptions made, are validated using steady- and unsteady-flow measurements combined with flow visualizations. In order to evaluate the effective impact of the revised theory, we then present an application to a simple mechanical model of the vocal cords derived from the classical two-mass model. As expected, implementation of a moving separation point appears to be of great importance for the modeling of glottal signals. It is further shown that the numerical model coupled with a more realistic description of the vocal cord collision can lead to signals surprisingly close to those observed in real speech by inverse filtering

Theoretical and experimental study of quasisteady-flow separation within the glottis during phonation : application to a modified two-mass model

Most flow models used in numerical simulation of voiced sound production rely, for the sake of simplicity, upon a certain number of assumptions. While most of these assumptions constitute reasonable first approximations, others appear more doubtful. In particular, it is implicitly assumed that the air flow through the glottal channel separates from the walls at a fixed point. Since this assumption appears quite unrealistic, and considering that the position of the separation point is an important parameter in phonation models, in this paper a revised fluid mechanical description of the air flow through the glottis is proposed, in which the separation point is allowed to move. This theoretical model, as well as the assumptions made, are validated using steady- and unsteady-flow measurements combined with flow visualizations. In order to evaluate the effective impact of the revised theory, we then present an application to a simple mechanical model of the vocal cords derived from the classical two-mass model. As expected, implementation of a moving separation point appears to be of great importance for the modeling of glottal signals. It is further shown that the numerical model coupled with a more realistic description of the vocal cord collision can lead to signals surprisingly close to those observed in real speech by inverse filtering

Non-Hermitian physics and PT symmetry

In recent years, notions drawn from non-Hermitian physics and parity–time (PT) symmetry have attracted considerable attention. In particular, the realization that the interplay between gain and loss can lead to entirely new and unexpected features has initiated an intense research effort to explore non-Hermitian systems both theoretically and experimentally. Here we review recent progress in this emerging field, and provide an outlook to future directions and developments