Полученные методом электростатического формования нанокомпозиты нейлона 6, 12 и клоизита 30B для стоматологического применения

In this study, nylon-nanoclay fibers were prepared by electrospinning. The electrospun membranes were characterized by fourier transform infra red spectroscopy (FTIR), electron microscopy, X-ray diffraction studies (XRD), contact angle and differential scanning calorimetry (DSC) analyses. The incorporation of nanoclay (Cloisite 30B) to the electrospun membrane which was confirmed by transmission electron microscopy (TEM) showed improvements in the overall properties of the membrane. Antimicrobial studies carried out using Enterococcus faecalis and Candida albicans showed that antimicrobial activity of the membrane with higher clay loading was comparable to that of commercially available dental antiseptic, even though lower clay concentrations did not show antimicrobial effects. The incremental addition of the Cloisite 30B resulted in significant increase in the antimicrobial activity and hydrophobicityВ работе были получены методом электростатического формования и исследованы волокна композита нейлона и наноглины (клоизит 30B). Мембраны были охарактеризованы с помощью ИК-спектроскопии, рентгеноструктурного анализа, анализа контактных углов и дифференциальной сканирующей калориметрии. Показано, что включение наноглины в мембраны, которое было подтверждено с помощью просвечивающей электронной микроскопии, привело к улучшению свойств мембран. Исследования, выполненные с использованием Enterococcus faecalis и Candida albicans, установили антимикробную активность мембран с высоким содержанием глины, сопоставимую с активностью имеющихся в продаже антисептиков, используемых в стоматологии. В то же время мембраны с низким содержанием глины не проявляли антимикробных свойств. Увеличение содержания клоизита 30B приводило к существенному увеличению антибактериальной активности и гидрофобности мембра

Dose-dependent effects of gamma irradiation on the materials properties and cell proliferation of electrospun polycaprolactone tissue engineering scaffolds

Electrospun membranes of polycaprolactone are widely used for biomedical applications like wound dressings and tissue engineering scaffolds. It is important to sterilize this material using the most accepted method, the gamma irradiation. In this study, we have evaluated the sterilizability of electrospun polycaprolactone membranes with gamma radiation of varying doses. The irradiated materials were assessed for the changes in morphology, crystallinity, surface degradation, hydrophilicity, mechanical property, sterility and the cell proliferation. Our results demonstrate that electrospun polycaprolactone can be effectively sterilized by gamma irradiation, however a higher dose of radiation affect the materials properties. The irradiated membranes showed improved hydrophilicity and fibroblast cell proliferation.Department of Biotechnology (DBT), Government of India, New Delhi.MSUB IPLSARE program.http://www.tandfonline.com/loi/gpom202016-01-31hb201

Growth factor loaded in situ photocrosslinkable poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/gelatin methacryloyl hybrid patch for diabetic wound healing

© 2020 The Authors Management of chronic diabetic ulcers remains as a major challenge in healthcare which requires extensive multidisciplinary approaches to ensure wound protection, management of excess wound exudates and promoting healing. Developing wound healing patches that can act as a protective barrier and support healing is highly needed to manage chronic diabetic ulcers. In order to boost the wound healing potential of patch material, bioactive agents such as growth factors can be used. Porous membranes made of nanofibers generated using electrospinning have potential for application as wound coverage matrices. However, electrospun membranes produced from several biodegradable polymers are hydrophobic and cannot manage the excess exudates produced by chronic wounds. Gelatin-methacryloyl (GelMA) hydrogels absorb excess exudates and provide an optimal biological environment for the healing wound. Epidermal growth factor (EGF) promotes cell migration, angiogenesis and overall wound healing. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) membranes provide microbial, thermal and mechanical barrier properties to the wound healing patch. Herein, we developed a biodegradable polymeric patch based on the combination of mechanically stable electrospun PHBV, GelMA hydrogel and EGF for promoting diabetic wound healing. In vitro and in vivo studies were carried out to evaluate the effect of developed patches on cell proliferation, cell migration, angiogenesis and wound healing. Our results showed that EGF loaded patches can promote the migration and proliferation of multiple types of cells (keratinocytes, fibroblasts and endothelial cells) and enhance angiogenesis. In situ development of the patch and subsequent in vivo wound healing study in diabetic rats showed that EGF loaded patches provide rapid healing compared to control wounds. Interestingly, 100 ng EGF per cm2 of the patches was enough to provide favourable cellular response, angiogenesis and rapid diabetic wound healing. Overall results indicate that EGF loaded PHBV-GelMA hybrid patch could be a promising approach to promote diabetic wound healing

Table 2: Bacterial diversity in Ny-Ålesund, Spitzbergen

We describe the antibiotic resistance profiling of bacterial isolates collected from Ny-Alesund, Arctic, as part of the Indian Arctic Summer Expedition 2009. It was interesting to note that the bacterial isolates collected from the Arctic showed multidrug resistance. 32% of the isolates were found to be multi- drug resistant with several combinations of antibiotics. The 16S rRNA sequencing results shows a diverse group of bacteria belonging to Phyla Proteobacteria, Actinobacteria and Bacteriodetes and their relatedness was studied by phylogenetic analysis. While analysing the plasmid profiling, the most resistant two strains of Pseudomonas migulae showed multiple plasmids of varying sizes ~5.2-5.3 kb and ~9.5 kb. The extent and frequency of multidrug resistance in the polar bacteria deserves close monitoring and efforts to understand the various molecular mechanisms of drug resistance and control the spread of antibiotic resistance in polar environment is called for

Исследование противомикробных барьерных свойств и совместимости с кровью полученных электропрядением нанокомпозитных скаффолдов поли-ƹ-капролактон/оксид цинка

Electrospun poly-ƹ-caprolactone/zinc oxide (PCL/ZnO) nanocomposite scaffolds were reported for tissue engineering and wound healing applications. Wound coverage materials should have good barrier property against invading microbes. Since wound coverage materials and tissue engineering scaffolds are in direct contact with blood, such materials should be blood compatible. Thus, blood compatibility of the fabricated scaffolds has been tested by RBC and WBC aggregation studies. Hemolysis assay and platelet activation study were also carried out. This study is promising in the sense that the fabricated scaffolds showed excellent microbial barrier property and were highly compatible with RBC and WBC and did not induce haemolysis. However, need to be vigilant regarding the possible platelet aggregation that can happen at higher concentrations of ZnO nanoparticlesИзучены полученные электропрядением нанокомпозитные скаффолды поли-ƹ-капролактон/оксид цинка (PCL/ZnO), которые могут быть использованы в тканевой инженерии и при лечении ран. Материалы раневых покрытий должны иметь высокие противомикробные барьерные свойства. Поскольку материалы раневых покрытий и тканеинженерные скаффолды находятся в прямом контакте с кровью, такие материалы должны быть кровесовместимыми. Поэтому была исследована совместимость полученных скаффолдов с кровью путем оценки агрегации эритроцитов и лейкоцитов. Также была проведена гемолизная проба и исследование активации тромбоцитов. Исследование показало хорошие противомикробные барьерные свойства и высокую совместимость скаффолдов с эритроцитами и лейкоцитами, а также отсутствие индукции гемолиза. Однако при высоких концентрациях наночастиц ZnO может отмечаться агрегация тромбоцито

Biopolymers and their industrial applications [electronic resource] : from plant, animal, and marine sources, to functional products /

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Bionanocomposite films based on potato, tapioca starch and chitosan reinforced with cellulose nanofiber isolated from turmeric spent

We study the nonequilibrium dynamics of a Bose-Einstein condensate that is split in a harmonic trap by turning up a periodic optical lattice potential. We evaluate the dynamical evolution of the phase coherence along the lattice and the number fluctuations in individual lattice sites within the stochastic truncated Wigner approximation when several atoms occupy each site. We show that the saturation of the number squeezing at high lattice strengths, which was observed in recent experiments by Orzel et al. [Science 291, 2386 (2001)] can be explained by the nonadiabaticity of the splitting

A study on short term treatment response of connective tissue disease related interstitial lung disease

Background: Interstitial lung disease is a major cause of morbidity and mortality in patients suffering from connective tissue diseases. Early detection and prompt recognition of symptoms with appropriate treatment is necessary for effective control of the disease and for better prognosis and long-term survival. Aim: To study the short-term treatment response of patients with connective tissue disease-related interstitial lung disease. Objectives: The purpose of this study was to evaluate the treatment response of connective tissue disease-related interstitial lung disease patients treated with intravenous pulses of cyclophosphamide (CYP) based on improvements in lung function: forced vital capacity (FVC)% predicted, forced expiratory volume in the first second (FEV1)% predicted, dyspnea Borg scale and 6-minute walk test. To determine the factors affecting the treatment outcomes like age, sex, duration of the connective tissue disease, type of connective tissue disease, HRCT type, and presence of PAH. Methods: A cohort study was conducted in the Department of General Medicine, Rheumatology, and Pulmonology at the Amala Institute of Medical Sciences, Thrissur, and Kerala from November 2015 to June 2017 that evaluated 74 patients having connective tissue disease-related ILD. A detailed history and a thorough clinical examination were done.&nbsp

Development of titanium dioxide nanowire incorporated poly(vinylidene fluoride–trifluoroethylene) scaffolds for bone tissue engineering applications

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