A multidisciplinary study of an exceptional prehistoric waste dump in the mountainous inland of Calabria (Italy) : implications for reconstructions of prehistoric land use and vegetation in Southern Italy

The mountainous inland of northern Calabria (Southern Italy) is known for its sparse prehistoric human occupation. Nevertheless, a thorough multidisciplinary approach of field walking, geophysical survey and invasive research led to the discovery of a major archaeological archive. This archive concerns a rich multi-phased dump, spanning about 3000 years (Late Neolithic to Late Imperial Roman Age) and holding two Somma-Vesuvius tephra. Of these, the younger is a distinct layer of juvenile tephra from the Pompeii eruption, while the older concerns reworked tephra from the Bronze Age AP2 eruption (ca. 1700 cal. yr BP). The large dump contains abundant ceramics, faunal remains and charcoal, and most probably originated through long-continued deposition of waste in a former gully like system of depressions. This resulted in an inversed, mound-like relief, whose anthropogenic origin had not been recognized in earlier research. The tephras were found to be important markers that support the reconstruction of the occupational history of the site. The sequence of occupational phases is very similar to that observed in a recent palaeoecological study from nearby situated former lakes (Lago Forano/Fontana Manca). This suggests that this sequence reflects the more regional occupational history of Calabria, which goes back to ca. 3000 BC. Attention is paid to the potential link between this history and Holocene climatic phases, for which no indication was found. The history deviates strongly from histories deduced from the few, but major palaeorecords elsewhere in the inlands of Southern Italy (Lago Grande di Monticchio and Lago Trifoglietti). We conclude that major regional variation occurred in prehistoric land use and its impacts on the vegetation cover of Southern Italy, and studies of additional palaeoarchives are needed to unravel this complex history. Finally, shortcomings of archaeological predictive models are discussed and the advantages of truly integrated multidisciplinary research

Protohistoric briquetage at Puntone (Tuscany, Italy):principles and processes of an industry based on the leaching of saline lagoonal sediments

A protohistoric (c.10th-5th c. BC) briquetage site at Puntone (Tuscany, Italy) was studied to unravel the salt production processes and materials involved. Geophysical surveys were used to identify kilns, pits, and dumps. One of these pits and a dump were excavated, followed by detailed chemical and physical analyses of the materials encountered. The pit had been used for holding brine, obtained by leaching of lagoonal sediment over a sieve, that afterwards was discarded to form large dumps. Phases distinguished indicate that the pit filled with fine sediment and was regularly "cleaned." The presence of ferroan-magnesian calcite in the pit fill testifies to the prolonged presence of anoxic brine. The production processes could be reconstructed in detail by confronting the analytical results with known changes in composition of a brine upon evaporation. These pertain in particular to the accumulation of "bitterns" and increased B (boron) concentrations in a residual brine. Both could be traced in the materials studied, and were found to be far more indicative than the ubiquitously studied concentrations of Cl and Na.</p

Protohistoric briquetage at Puntone (Tuscany, Italy) : principles and processes of an industry based on the leaching of saline lagoonal sediments

A protohistoric (c.10th–5th c. BC) briquetage site at Puntone (Tuscany, Italy) was studied to unravel the salt production processes and materials involved. Geophysical surveys were used to identify kilns, pits, and dumps. One of these pits and a dump were excavated, followed by detailed chemical and physical analyses of the materials encountered. The pit had been used for holding brine, obtained by leaching of lagoonal sediment over a sieve, that afterwards was discarded to form large dumps. Phases distinguished indicate that the pit filled with fine sediment and was regularly “cleaned.” The presence of ferroan‐magnesian calcite in the pit fill testifies to the prolonged presence of anoxic brine. The production processes could be reconstructed in detail by confronting the analytical results with known changes in composition of a brine upon evaporation. These pertain in particular to the accumulation of “bitterns” and increased B (boron) concentrations in a residual brine. Both could be traced in the materials studied, and were found to be far more indicative than the ubiquitously studied concentrations of Cl and Na

New approach to privacy-preserving clinical decision support systems for HIV treatment

Background: HIV treatment prescription is a complex process. Clinical decision support systems (CDSS) are a category of health information technologies that can assist clinicians to choose optimal treatments based on clinical trials and expert knowledge. The usability of some CDSSs for HIV treatment would be significantly improved by using the knowledge obtained by treating other patients. This knowledge, however, is mainly contained in patient records, whose usage is restricted due to privacy and confidentiality constraints. Methods: A treatment effectiveness measure, containing valuable information for HIV treatment prescription, was defined and a method to extract this measure from patient records was developed. This method uses an advanced cryptographic technology, known as secure Multiparty Computation (henceforth referred to as MPC), to preserve the privacy of the patient records and the confidentiality of the clinicians’ decisions. Findings: Our solution enables to compute an effectiveness measure of an HIV treatment, the average time-to-treatment-failure, while preserving privacy. Experimental results show that our solution, although at proof-of-concept stage, has good efficiency and provides a result to a query within 24 min for a dataset of realistic size. Interpretation: This paper presents a novel and efficient approach HIV clinical decision support systems, that harnesses the potential and insights acquired from treatment data, while preserving the privacy of patient records and the confidentiality of clinician decisions

Salt or fish (or salted fish)? The Bronze Age specialised sites along the Tyrrhenian coast of Central Italy : new insights from Caprolace settlement

In 2017, an excavation led by the Groningen Institute of Archaeology and in collaboration with the Tor Vergata University of Rome, took place on two small islands in the Caprolace lagoon (Sabaudia, Italy), where Middle Bronze Age layers had previously been reported. Combining the results of an environmental reconstruction of the surroundings and a detailed study of the pottery assemblages, we were able to trace a specialised area on the southern island, in all probability devoted to salt production by means of the briquetage technique. The latter basically consists of boiling a brine through which a salt cake is obtained. The technique was widespread all over Europe, from Neolithic to Roman Times. Since the evidence points to an elite-driven workshop, this result has deep implications for the development of the Bronze Age socio-economic framework of Central Italy. Pottery evidence also suggests that in the Bronze Age sites along the Tyrrhenian coast of Central Italy where briquetage has already been hypothesised, more complex processes may have taken place. On the northern island, we collected a large number of so-called pedestals, which are characteristic features of briquetage, while chemical analyses point to salt or fish sauce production, like the roman liquamen, in a Middle Bronze Age domestic context

Protohistoric briquetage at Puntone (Tuscany, Italy): principles and processes of an industry based on the leaching of saline lagoonal sediments

A protohistoric (c.10th–5th c. BC) briquetage site at Puntone (Tuscany, Italy) was studied to unravel the salt production processes and materials involved. Geophysical surveys were used to identify kilns, pits, and dumps. One of these pits and a dump were excavated, followed by detailed chemical and physical analyses of the materials encountered. The pit had been used for holding brine, obtained by leaching of lagoonal sediment over a sieve, that afterwards was discarded to form large dumps. Phases distinguished indicate that the pit filled with fine sediment and was regularly “cleaned.” The presence of ferroan‐magnesian calcite in the pit fill testifies to the prolonged presence of anoxic brine. The production processes could be reconstructed in detail by confronting the analytical results with known changes in composition of a brine upon evaporation. These pertain in particular to the accumulation of “bitterns” and increased B (boron) concentrations in a residual brine. Both could be traced in the materials studied, and were found to be far more indicative than the ubiquitously studied concentrations of Cl and Na

Chromatinized protein kinase C-Θ directly regulates inducible genes in epithelial to mesenchymal transition and breast cancer stem cells

Epithelial to mesenchymal transition (EMT) is activated during cancer invasion and metastasis, enriches for cancer stem cells (CSCs), and contributes to therapeutic resistance and disease recurrence. Signal transduction kinases play a pivotal role as chromatin- anchored proteins in eukaryotes. Here we report for the first time that protein kinase C-theta (PKC-θ) promotes EMT by acting as a critical chromatin-anchored switch for inducible genes via transforming growth factor β (TGF-β) and the key inflammatory regulatory protein NF-κB. Chromatinized PKC-θ exists as an active transcription complex and is required to establish a permissive chromatin state at signature EMT genes. Genome-wide analysis identifies a unique cohort of inducible PKC-θ-sensitive genes that are directly tethered to PKC-θ in the mesenchymal state. Collectively, we show that cross talk between signaling kinases and chromatin is critical for eliciting inducible transcriptional programs that drive mesenchymal differentiation and CSC formation, providing novel mechanisms to target using epigenetic therapy in breast cancer