INFLUENCE OF BRADYRHIZOBIUM AND TWO GLOMUS SPECIES ON THE GROWTH AND YIELD OF SOYBEAN

A screenhouse experiment was conducted to investigate the effect of Bradyrhizobium japonicum on the response of soybean to inoculation with two species of mycorrhiza (Glomus mosseae and Glomus deserticola). The study was carried out in a screenhouse with soybean as test crop. The two species of arbuscular mycorrhiza fungi (AMF) were inoculated to the potted soil with or without B. japonicum. Single super phosphate (SSP) and zero amendment served as conventional and absolute control respectively. The experimental design was randomized complete block with 4 replicates. AMF/Bradyrhizobium interaction increased mycorrhizal fungi root colonization significantly (p = 0.05) by at least 35.9% at early growth stage (3 weeks after planting (WAP)) and 59.5% at later growth stage (9 WAP). G mosseae/Bradyrhizobium interaction significantly increased N and P uptakes by 68.9 and 80.0%, respectively, as well as plant height, number of leaves, number of branches, canopy spread and leaf area between 2 and 5 WAP. Soybean biomass increased significantly due to interaction of G mosseae and Bradyrhizobium by 42.2-53.4% between 3 and 9 WAP and nodule weight increased by 61.9-93.3% between 6 and 9 WAP. Grain yield per plant was similar in all AMF treatments and SSP but less in sole Bradyrhizobium inoculation by 37.5% and in control by 33.3%. AMF/Bradyrhizobium interactions produced higher N by up to 81% in the residual soil and the P content was similar to SSP but higher than in control by up to 32.3%. Interaction of G mosseae and Bradyrhizobium increased spores of mycorrhizal fungi in the soil by 41% at 3 WAP and 74.7% at 9 WAP. It was concluded that although Bradyrhizobium had a positive and synergistic influence on the activities of the two species of mycorrhizal fungi inoculated on soybean, the influence was, however, more pronounced on G mosseae than G deserticola

IMPROVEMENT OF TOMATO SEED LONGEVITY USING SOME DRY SEED PRE-STORAGE TREATMENTS

Pre-storage treatment to retard seed deterioration could either involve application of synthetic chemicals or plant/organic products. This study was conducted to determine the efficacy of some organic dry seed treatments and inorganic chemical treatments on the viability of stored seeds of four tomato (Solanum lycopersicum) varieties. The study was a factorial experiment fixed in completely randomized design. It comprised of four tomato varieties (Roma, UC-82, 2-lobes and 3-lobes) and five dry seed treatment materials (neem leaves, scent leaves, red chili, bleaching and Apron plus powders) in three replicates. Seeds stored without any treatment were used as the control. The tomato seeds were stored for 240 days under ambient condition (average temperature 30ºC and 75% relative humidity). Half-life of the stored seeds ranged from 286 to 2105 days. Generally, organic treatment doubled the longevity when compared to the control treatment. Performance of seed treatments depended on tomato seed variety. Use of neem leaf and scent leaf powders had greater potentials to replace the commercial inorganic seed treatment materials for tomato seed preservation under the same environmental conditions.   &nbsp

Some aspects of the Seed Germination and Seedling Growth of two Savanna tree Species: Prosopis africana and Dialium guineense

Studies were made on some aspects of the seed germination and seedling growth of two multipurpose trees. These include the effect of pre-sowing treatments, seed sizes and gibberellic acid on the germination of seeds and seedling growth. The tree species include Prosopis africana (Guill. & Perr.) Taub. and Dialium guineense (Wild). Two seed sizes designated small- size (Ss) and Big-size (Bs) were identified in the seed. The effect of gibberellic acid (GA3) had a greater significance effect (P < 0.05) on seed germination of both D. guineense and P. africana seeds. The big size seeds had a significant effect (P < 0.05) on the seed germination when compared to the small size seeds. The hydration/dehydration, pre-sowing treatments on the seeds did not have any significant effects on germination

Population of Fungi Associated with Seed Germination after Storage and Seed Treatment

Fungi growth occurs in seeds as a result of environmental conditions (high relative humidity, suitable temperature and high level of seed moisture content). But pathogen free healthy seeds are required for plant propagation. Microbial and insect attack affect seeds throughout the life cycle of the plant (Islam et al., 2009). Fungi are the most common microbes that affect seed germination and they can be both saprophytic and pathogenic. Hence, the aim of this experiment is to determine the cultural and morphological characteristics and population of fungi associated with the germination of some legume seeds after storage and scarification

PHYSIOLOGICAL AND YIELD RESPONSE OF SOME UPLAND RICE VARIETIES TO RE-WATERING AFTER IMPOSED SOIL MOISTURE STRESS

A pot experiment was conducted in the Screen house of Federal University of Agriculture, Abeokuta, October, 2011 (late dry season) to study drought recovery ability of 13 upland rice varieties exposed to soil moisture stress (20 days) at three growth stages (vegetative, reproductive and grain filling stage). The experiment was in completely randomized design, with three replicates. Under moisture stress significantly higher growth recovery, more erect canopy and flatter leaf surface were obtained in all the rice varieties at vegetative growth stage than other growth stages with increasing duration of re-watering. Under stress condition NERICA 4 maintained a significantly higher leaf area (27.50 cm2 and 40.18 cm2), plant height (53.45 cm and 67.62 cm) and number of tillers (1.67 and 1.67), but with a depressed number of leaf, slanted leaf posture and curved leaf especially during the later stage of its growth (Reproductive and grain filling stage respectively). It could be concluded that NERICA 4 had higher recovery ability than other rice varieties in drought prone upland ecology

ROOT RESPONSE OF SOME SELECTED RICE VARIETIES TO SOIL MOISTURE STRESS AT DIFFERENT PHENOLOGICAL STAGES

Physiological adjustment in plant root system is a determinant for survival and crop productivity in situation of moisture stress. A screen house experiment was conducted to access response of rice roots to moisture stress. Thirteen  varieties of rice comprising six NERICAs, WAB 56-104, CG 14, ART26-3-1-B, AC 103549, MOROBEREKAN, ART19-25-1-B and a local check (OFADA) were subjected to twenty-day moisture stress once at  each phenological stage. Results indicated that root growth generally showed preference over shoot growth. Moisture stress did not affect root volume (RV), deep root numbers (DRN), root dry weight (RDW) and root depth (RD) of all the rice varieties at reproductive stage. CG14 however recorded 67.6% increase in RD at this stage while NERICA 3, CG14 and OFADA recorded an increase in root depth: shoot length. At vegetative and grain filling stages, RV, DRN, RDW, RD, and RMC were significantly (p&lt; 0.05) increased by moisture stress in most rice varieties. NERICA2, NERICA7, ART26-3-1-B, MOROBEREKAN and WAB56-104 however recorded 54%, 76.5%, 72.7%, 57.1%, and 56.3% significant reduction in DRN respectively at vegetative stage. Correlation analysis showed that plant height, leaf area, and number of tillers depend highly on, RD, RV, RDW and deep root weight. Therefore, attention should be focused on these parameters in selection for moisture stress tolerance in rice

Diversidad microbiana y actividad de enzimas digestivas en el intestino de las lombrices de tierra en los aserraderos de Abeokuta, Nigeria

The growing demand for wood has resulted in large volumes of wood wastes that are daily released to the soil from the activities of sawmills in South-Western Nigeria. In an attempt to setup a bioremediation model for sawdust, this study therefore aimed at evaluating microbial diversity, and the level of digestive enzymes in the gut of earthworms (Eudrilus eugeniae, Libyodrilus violaceous and Hyperiodrilus africanus) of sawmill origin. Four major sawmills located in Abeokuta (7o9'12" N - 3o19'35" E), namely Lafenwa, Sapon, Isale-Ake and Kotopo sawmills were used for this study. The arboretum of the Federal University of Agriculture, Abeokuta was used as control. Gut microbial analysis was carried out using the pour-plate method while digestive enzyme activities in the earthworm guts were done by the spectrophotometric method. Higher microbial counts (28.5±0.1x103-97.0±0.1x103cfu for bacteria and 7.0±0.1x103-96.0±0.1x103cfu for fungi) and microbial diversity were recorded in the gut of earthworms of the sawmill locations than those of the control site (17.5±0.1x103cfu for bacteria and 4.5±0.1x103cfu for fungi). Streptococcus mutans and Proteus spp. were common in the gut of E. eugeniae, and L. violaceous from the study sawmills, while Streptococcus mutans were also identified in H. africanus, but absent in the gut of E. eugeniae from the control site. Cellulase (48.67±0.02mg/g) and lipase (1.81±0.01mg/g) activities were significantly higher (p&lt;0.05) in the gut of earthworms from the control site than those of the study sawmills. Furthermore, amylase (α and β) activity was highest in the gut of earthworms from the sawmills. Variations observed in the gut microbial and digestive enzyme activities of earthworms from the study sawmills as compared to the control site suggests that earthworms, especially E. eugeniae, could be a better organism for use as bioremediator of wood wastes.La creciente demanda de madera ha resultado en grandes volúmenes de residuos de madera que se lanzan diariamente a la tierra, procedentes de las actividades de los aserraderos en el suroeste de Nigeria. Se realizó este estudio en un intento de configurar un modelo de biorremediación de aserrín, con el objetivo de evaluar la diversidad microbiana y el nivel de las enzimas digestivas en el intestino de las lombrices de tierra (Eudrilus eugeniae, Libyodrilus violáceo y Hyperiodrilus africanus) de origen aserradero. Para este estudio se utilizaron cuatro grandes aserraderos ubicados en Abeokuta (7°9'12 '' N - 3°19'35 '' E), a saber: Lafenwa, Sapon, Isale-Ake y Aserraderos Kotopo. El arboreto de la Universidad Federal de Agricultura, Abeokuta se utilizó como control. El análisis microbiano se llevó a cabo utilizando el método de vertido de placa, mientras que las actividades de enzimas digestivas en los intestinos de las lombrices se realizaron por el método espectrofotométrico. Los recuentos microbianos más altos fueron 28.5±97.0±0.1x103-0.1x103cfu para las bacterias y 7.0±0.1x103-96.0±0.1x103cfu para los hongos y la diversidad microbiana registrada en el intestino de las lombrices de tierra de los lugares de aserraderos y las del sitio de control fueron: 17.5±0.1x103cfu para bacterias y 4.5±0.1x103cfu para hongos. Streptococcus mutans y Proteus spp. eran comunes en el intestino de E. eugeniae, y L. violaceous de los aserraderos de estudio, mientras que también se identificaron Streptococcus mutans en H. africanus, que estuvieron ausentes en el intestino de E. eugeniae en el sitio de control. Actividades de celulasa (48.67±0.02mg/g) y lipasa (1.81±0.01mg/g) fueron significativamente mayores (p&lt;0.05) en el intestino de las lombrices de tierra en el sitio de control que las de los aserraderos de estudio. Por otra parte, la actividad de la amilasa (α y β) fue mayor en el intestino de las lombrices de tierra de los aserraderos. Las variaciones observadas en la microbiota intestinal y la actividad de las enzimas digestivas de las lombrices de tierra de los aserraderos de estudio, en comparación con el sitio de control, sugieren que las lombrices de tierra, especialmente E. eugeniae, podrían ser un mejor organismo para su uso como biorremediador de residuos de madera

Microbial diversity and digestive enzyme activities in the gut of earthworms found in sawmill industries in Abeokuta, Nigeria

The growing demand for wood has resulted in large volumes of wood wastes that are daily released to the soil from the activities of sawmills in South-Western Nigeria. In an attempt to setup a bioremediation model for sawdust, this study therefore aimed at evaluating microbial diversity, and the level of digestive enzymes in the gut of earthworms (Eudrilus eugeniae, Libyodrilus violaceous and Hyperiodrilus africanus) of sawmill origin. Four major sawmills located in Abeokuta (7o9’12” N - 3o19’35” E), namely Lafenwa, Sapon, Isale-Ake and Kotopo sawmills were used for this study. The arboretum of the Federal University of Agriculture, Abeokuta was used as control. Gut microbial analysis was carried out using the pour-plate method while digestive enzyme activities in the earthworm guts were done by the spectrophotometric method. Higher microbial counts (28.5±0.1x10³-97.0±0.1x10³cfu for bacteria and 7.0±0.1x10³-96.0±0.1x10³cfu for fungi) and microbial diversity were recorded in the gut of earthworms of the sawmill locations than those of the control site (17.5±0.1x10³cfu for bacteria and 4.5±0.1x10³cfu for fungi). Streptococcus mutans and Proteus spp.were common in the gut of E. eugeniae, and L. violaceous from the study sawmills, while Streptococcus mutans were also identified in H. africanus, but absent in the gut of E. eugeniae from the control site. Cellulase (48.67±0.02mg/g) and lipase (1.81±0.01mg/g) activities were significantly higher (p<0.05) in the gut of earthworms from the control site than those of the study sawmills. Furthermore, amylase (α and β) activity was highest in the gut of earthworms from the sawmills. Variations observed in the gut microbial and digestive enzyme activities of earthworms from the study sawmills as compared to the control site suggests that earthworms, especially E. eugeniae, could be a better organism for use as bioremediator of wood wastes. Rev. Biol. Trop. 62 (3): 1241-1249. Epub 2014 September 01

Effects of Molasses and Storage Period on the Chemical, Microbial and Fermentation Characteristics of Guinea Grass - Cassava Leaves Silage

The study was conducted to determine the effects of molasses and storage periods on the chemical composition, microbial and fermentation characteristics of silage produced from guinea grass and cassava leaves mixture. Guinea grass was harvested at 2 months regrowth from an established pasture and cassava tops collected immediately after root harvest consisting of only the tops with the green stem and its leaf canopy. The forages were wilted and thoroughly mixed in the ratio of 70:30 (guinea grass: cassava leaves); mixed with molasses at the rate of 0, 2, 4 and 6 % and ensiled for 30 and 60 days respectively for fermentation. The DM, CP, NDF, HCN and tannin contents of the ensiled guinea grass and cassava leaves significantly reduced as the level of molasses addition and storage period increased. The acetic acid fermentation was high in the 0, 2, 4 and 6% molasses addition with values ranging from 40.2 to 42.4 g/kg DM while the lactic acid content was only 18.4 to 30.3 g/kg DM at the 0% molasses addition. However, lactic acid fermentation increased significantly as both the storage periods and molasses addition increased from 0 to 60 days. The highest bacterial count (8.4 log cfu/g) was recorded at the 0% molasses addition and this significantly reduced as molasses addition increased from 0 to 6% and as the ensiling periods elongate from 0 to 60 days. However, a sharp decline in fungi count was observed with increase in the percentage of molasses added. The bacteria isolated from the silage were Staphylococcus aureus, Streptococcus spp., Enterobacter spp., Proteus spp., Escherichia coli and Lactobacillus buchneri. It is concluded that ensiling mixture of guinea grass and cassava leaves with molasses increased lactic acid and suppressed the production of acetic and butyric acids with drastic reduction in microbial load.Keywords: Guinea grass, cassava, storage period, molasses, fermentation, chemical compositio