Emotion Recognition from Acted and Spontaneous Speech

Dizertační práce se zabývá rozpoznáním emočního stavu mluvčích z řečového signálu. Práce je rozdělena do dvou hlavních častí, první část popisuju navržené metody pro rozpoznání emočního stavu z hraných databází. V rámci této části jsou představeny výsledky rozpoznání použitím dvou různých databází s různými jazyky. Hlavními přínosy této části je detailní analýza rozsáhlé škály různých příznaků získaných z řečového signálu, návrh nových klasifikačních architektur jako je například „emoční párování“ a návrh nové metody pro mapování diskrétních emočních stavů do dvou dimenzionálního prostoru. Druhá část se zabývá rozpoznáním emočních stavů z databáze spontánní řeči, která byla získána ze záznamů hovorů z reálných call center. Poznatky z analýzy a návrhu metod rozpoznání z hrané řeči byly využity pro návrh nového systému pro rozpoznání sedmi spontánních emočních stavů. Jádrem navrženého přístupu je komplexní klasifikační architektura založena na fúzi různých systémů. Práce se dále zabývá vlivem emočního stavu mluvčího na úspěšnosti rozpoznání pohlaví a návrhem systému pro automatickou detekci úspěšných hovorů v call centrech na základě analýzy parametrů dialogu mezi účastníky telefonních hovorů.Doctoral thesis deals with emotion recognition from speech signals. The thesis is divided into two main parts; the first part describes proposed approaches for emotion recognition using two different multilingual databases of acted emotional speech. The main contributions of this part are detailed analysis of a big set of acoustic features, new classification schemes for vocal emotion recognition such as “emotion coupling” and new method for mapping discrete emotions into two-dimensional space. The second part of this thesis is devoted to emotion recognition using multilingual databases of spontaneous emotional speech, which is based on telephone records obtained from real call centers. The knowledge gained from experiments with emotion recognition from acted speech was exploited to design a new approach for classifying seven emotional states. The core of the proposed approach is a complex classification architecture based on the fusion of different systems. The thesis also examines the influence of speaker’s emotional state on gender recognition performance and proposes system for automatic identification of successful phone calls in call center by means of dialogue features.

Development of immunoassays for human urokinase

Radioimmune assays (RIA) and enzyme linked immune assays for measurement of pro-urokinase and the two active forms of the enzyme were developed. Polyclonal and monoclonal antibodies, with desired specificities against preselected synthetic regions of urokinase (UK), were obtained by immunization with the respective synthetic peptides and used to develop RIA for zymogen and the two activated forms of UK

Feedback in separated flows over symmetric airfoils

For a flow over an airfoil with laminar separation, a feedback cycle may exist whereby a Kelvin-Helmholtz instability wave emanating from the separation point on the airfoil surface grows along the shear layer and is diffracted as it interacts with the sharp trailing edge of the airfoil, causing acoustic radiation which, in turn, propagates upstream and regenerates the initial instability wave. The analysis is restricted to the high frequency limit. Solutions to the boundary-value problem are obtained using the slowly varying approximation and the method of matched asymptotic expansions. Resonant solutions exist for certain discrete values of the Reynolds and Strouhal numbers. The results are discussed and compared with available data

Cataplexy versus Pseudoseizure : A Case Study

Cataplexy is frequently misdiagnosed as pseudo seizures. This case study about a young patient shows the necessary steps needed to accurately diagnose and treat cataplexy as well as understand the differences between narcolepsy Type I and Type II

A finite-difference, frequency-domain numerical scheme for the solution of the linearized unsteady Euler equations

A numerical method is developed for solving periodic, three-dimensional, vortical flows around lifting airfoils in subsonic flow. The first-order method, that is presented, fully accounts for the distortion effects of the nonuniform mean flow on the convected upstream vortical disturbances. The unsteady velocity is split into a vortical component which is a known function of the upstream flow conditions and the Lagrangian coordinates of the mean flow, and an irrotational field whose potential satisfies a nonconstant-coefficient, inhomogeneous, convective wave equation. Using an elliptic coordinate transformation, the unsteady boundary value problem is solved in the frequency domain on grids which are determined as a function of the Mach number and reduced frequency. Extensive comparisons are made with known solutions to unsteady vortical flow problems, and it is seen that the agreement is generally very good for reduced frequencies ranging from 0 up to 4

Measuring the metastatic potential of cancer cells

Cancer cells must secrete proteolytic enzymes to invade adjacent tissues and migrate to a new metastatic site. Urokinase (uPA) is a key enzyme related to metastasis in cancers of the lung, colon, gastric, uterine, breast, brain, and malignant melanoma. A NASA technology utilization project has combined fluorescence microscopy, image analysis, and flow cytometry, using fluorescent dyes, and urokinase-specific antibodies to measure uPA and abnormal DNA levels (related to cancer cell proliferation) inside the cancer cells. The project is focused on developing quantitative measurements to determine if a patient's tumor cells are actively metastasizing. If a significant number of tumor cells contain large amounts of uPA (esp. membrane-bound) then the post-surgical chemotherapy or radiotherapy can be targeted for metastatic cells that have already left the primary tumor. These analytical methods have been applied to a retrospective study of biopsy tissues from 150 node negative, stage 1 breast cancer patients. Cytopathology and image analysis has shown that uPA is present in high levels in many breast cancer cells, but not found in normal breast. Significant amounts of uPA also have been measured in glioma cell lines cultured from brain tumors. Commercial applications include new diagnostic tests for metastatic cells, in different cancers, which are being developed with a company that provides a medical testing service using flow cytometry for DNA analysis and hormone receptors on tumor cells from patient biopsies. This research also may provide the basis for developing a new 'magic bullet' treatment against metastasis using chemotherapeutic drugs or radioisotopes attached to urokinase-specific monoclonal antibodies that will only bind to metastatic cells