and TNF-&alpha Productions of E. coli Lipopolysaccharide-Induced Periodontitis Model on Rats

Periodontaldisease,a commoninflammatoryoraldiseaseinvolvedperiodontaltissues,has beenlinked with the evidenceof some systemicdisorders. Recently,periodontaldisease has beensuspected as a trigger of systemic disorders. Penetration of bacterial products, such as lipopolysaccharide (LPS)may reach into deeper periodontal tissues. Therefore there may affect-systemic blood and cytokines production. Interleukin-1~ (IL- 1~) and Tumour Nuclear Factor-a (TNF-a) are known as pro-inflammatory cytokines. The production of systemic IL-1~and TNF-aof E.coli lipopolysaccharide-induced periodontitis model on rats was investigated in this research. Fifteen male Wistar rats, aged 6-8 weeks used for this study were divided into 3 groups. For group 1 and 2, silk ligature 3/0 were inserted in interdental area between upper right molar 1 and 2. Firstand second group received solution containing lOllg/ml and 1mg/ml E.colilipopolysaccharide, respectively, mixed with 2%carboxymethylcellulose (CMe)diluted in lOO1l1of phosphate buffer saline (PBS).The solution was topically applied on gingivaltissues around the gingival sulcus, a single topical application of solution once per 2 days for 14 days. Untreated subjects were used as negative control. Onday 15, the blood was collected from vena orbitalis, and rats were sacrificed. The blood serum of each group was divided into 2 groups and cultured for 4 hours with or without 20111of 100ng/ml of E. coli LPS.ELISAtechniques were used to measure the cytokine productions of the supernatant. The data was ana lysed using Repeated Measure ANOVA.Jhis study showed that there was a significantincrease of IL-1~productionon low dose of LPScompared to control and high dose of LPSgroups (

Molecular typing of Candida albicans isolated from oral cavity of cancer patients using randomly amplified polymorphic DNA

To facilitate studies on the epidemiology of Candida, numerous phenotypic and genotypic typing systems have been developed. The polymerase chain reaction (PCR) can be adapted for pringerprinting microorganisms by using paired primers derived from previously characterized sequences for PCR amplification. The aim of the present study was to develop and use Randomly Amplified Polymorphic DNA (RAPD) for molecular genetic typing of Candida albicans. RAPD which is based on PCR with arbitrary short primers, was used to evaluate a panel of 9 Candida albicans that were isolated from oral cavity of cancer patients in Yogyakarta. Analysis of RAPDs with primer AT (5\u27 GCGCACGG 3\u27) gave 5 types of banding patterns. Primer NT (5\u27 CCCTAGGA 3\u27) yielded 8 types of banding patterns. These data indicate that many different substrains of Candida albicans can infect patients with cancer disease causing either oral colonization or oral candidosis. This study indicates that because of its ease and reproducibility, RAPD analysis is useful in providing genotypic characters for confirming the identities of stock isolates, for typing such as Candida albicans, as well as rapid identification of pathogenic fungi. Keywords: Candida â RAPD â genotypic typin

Systemic IL-1β and TNF-α Productions of E. coli Lipopolysaccharide-Induced Periodontitis Model on Rats

Periodontal disease, a common inflammatory oral disease involved periodontal tissues, has been linked with the evidence of some systemic disorders. Recently, periodontal disease has been suspected as a trigger of systemic disorders. Penetration of bacterial products, such as lipopolysaccharide (LPS) may reach into deeper periodontal tissues. Therefore there may affect systemic blood and cytokines production. Interleukin-1β (IL-1β) and Tumour Nuclear Factor-α (TNF-α) are known as pro-inflammatory cytokines. The production of systemic IL-1β and TNF-α of E. coli lipopolysaccharide-induced periodontitis model on rats was investigated in this research. Fifteen male Wistar rats, aged 6-8 weeks used for this study were divided into 3 groups. For group 1 and 2, silk ligature 3/0 were inserted in interdental area between upper right molar 1 and 2. First and second group received solution containing 10μg/ml and 1mg/ml E. coli lipopolysaccharide, respectively, mixedwith 2% carboxymethylcellulose (CMC) diluted in 100μl of phosphate buffer saline (PBS). The solution was topically applied on gingival tissues around the gingival sulcus, a single topical application of solution onceper 2 days for 14 days. Untreated subjects were used as negative control. On day 15, the blood was collected from vena orbitalis, and rats were sacrificed. The blood serum of each group was divided into 2 groups andcultured for 4 hours with or without 20μl of 100ng/ml of E. coli LPS. ELISA techniques were used to measure the cytokine productions of the supernatant. The data was analysed using Repeated Measure ANOVA. This study showed that there was a significant increase of IL-1β production on low dose of LPS compared to control and high dose of LPS groups (p<0.05). Whereas TNF-α not significantly showed increasing trend. The increasing trend of pro-inflammatory cytokine productions, such as IL-1β and TNF-α, on LPS-induced periodontitis model in this experiment supports the previous studies about the contribution of periodontal disease in the pathogenesis of systemic diseases

EFEK EKSTRAK DAUN TEH (Camellia sinensis) KONSENTRASI 0,5% TERHADAP KADAR sIgA PADA SALIVA PENDERITA GINGIVITIS

Enhanced humoral immune response (sIgA) can be caused by gingivitis. A study has been conducted on the effect of tea leaf extract (Camellia sinensis) at a concentration of 0,5% as mouth-wash on salivary sIgA level on gingivitis patients. Ten patients (age= 18-30 years) were divided into 2 groups so that five patients of the first group representes early gingivitis, and five patients of the second group represented normal gingiva. The gingival index by Loe and Silness (1963) was measured one day before treatment and five days after treatment. The first group was given tea leaf extract solution (0,5%) and the others were given aquabidest as control. Five ml of each solution was mouth washed for 5 seconds and this was repeated two times in the morning and at night before sleeping. All treatments were repeated for five days. Saliva samples were collected one day before treatment and five days after treatment. The sIgA level was measured using sIgA ELISA kit (Salimetrics LLC). Optical Density was read on standard plate at 450 nm. The results showed a significant decrease in the salivary sIgA level and in the severity of gingivitis by mouth-washing with a tea leaf extract at a concentration 0.5%

EFEK EKSTRAK DAUN TEH (Camellia sinensis) KONSENTRASI 0,5% TERHADAP KADAR sIgA PADA SALIVA PENDERITA GINGIVITIS

<p>Enhanced humoral immune response (sIgA) can be caused by gingivitis. A study has been conducted on the effect of tea leaf extract (Camellia sinensis) at a concentration of 0,5% as mouth-wash on salivary sIgA level on gingivitis patients. Ten patients (age= 18-30 years) were divided into 2 groups so that five patients of the first group representes early gingivitis, and five patients of the second group represented normal gingiva. The gingival index by Loe and Silness (1963) was measured one day before treatment and five days after treatment. The first group was given tea leaf extract solution (0,5%) and the others were given aquabidest as control. Five ml of each solution was mouth washed for 5 seconds and this was repeated two times in the morning and at night before sleeping. All treatments were repeated for five days. Saliva samples were collected one day before treatment and five days after treatment. The sIgA level was measured using sIgA ELISA kit (Salimetrics LLC). Optical Density was read on standard plate at 450 nm. The results showed a significant decrease in the salivary sIgA level and in the severity of gingivitis by mouth-washing with a tea leaf extract at a concentration 0.5%.</p

Unravelling strong electronic interlayer and intralayer correlations in a transition metal dichalcogenide

10.1038/s41467-021-27182-yNATURE COMMUNICATIONS12

Importance of Electronic Correlations and Unusual Excitonic Effects in Formamidinium Lead Halide Perovskites

10.1103/PhysRevX.8.021034PHYSICAL REVIEW X8

Electron transport and visible light absorption in a plasmonic photocatalyst based on strontium niobate.

Semiconductor compounds are widely used for photocatalytic hydrogen production applications, where photogenerated electron-hole pairs are exploited to induce catalysis. Recently, powders of a metallic oxide (Sr1-xNbO3, 0.033+δ and find that their bandgaps are ∼4.1 eV. Surprisingly, the carrier density of the conducting phase exceeds 1022 cm-3 and the carrier mobility is only 2.47 cm2 V-1 s-1. Contrary to earlier reports, the visible light absorption at 1.8 eV (∼688 nm) is due to the plasmon resonance, arising from the large carrier density. We propose that the hot electron and hole carriers excited via Landau damping (during the plasmon decay) are responsible for the photocatalytic property of this material under visible light irradiation

Dual phases of crystalline and electronic structures in the nanocrystalline perovskite CsPbBr3

10.1038/s41427-019-0170-6NPG ASIA MATERIALS1117