Electron paramagnetic resonance of gamma-irradiated single crystals of 3-nitroacetanilide

WOS: 000255813300003The electron paramagnetic resonance of single crystals of 3-nitroacetanilide has been observed and analyzed for different orientations of the crystal in the magnetic field, after being damaged at 300 K by gamma-irradiation. The crystals have been investigated between 123 and 300 K. The spectra were found to be temperature independent. The irradiation of 3-nitroacetanilide by gamma-rays produces radicals at the nitrogen atoms in the molecule. The principal values of the hyperfine coupling tensor of the unpaired electron and the principal values of the g-tensor were determined. (C) 2008 Elsevier Ltd. All rights reserved

An EPR study on single crystals of dimethyl-1,3-cyclohexanedione by gamma-rays

WOS: 000300852100001Single crystals of dimethyl-1,3-cyclohexanedione (C8H12O2) were produced by slow evaporation of the concentrated ethyl acetate solutions. These single crystals were exposed to Co-60 gamma-rays with a dose speed of 0.950 kGy/h at room temperature for 24, 48, and 72 h. The free radicals of the samples irradiated for 48 and 72 h were detected using electron paramagnetic resonance (EPR)-X-band spectrometer. EPR measurements were performed between 120 and 300 K. The sample irradiated for 48 h by gamma-rays was rotated in steps of 10 degrees at 298 K. The spectrum parameters were found to be dependent on the temperature. Two radicals were determined in the molecular structure irradiated. These radiation damage centers were called R1 and R2. The average values of g and the hyperfine coupling constants were calculated as follows: a(Ha) = 6.84 G, a(Hb) = 3.60 G, g = 2.0040 for R1, a(H) = 28 G, g = 2.0062 for R2.Scientific Research Projects coordination centers of Nigde; Selcuk University, TurkeyThis study was partially supported by the Scientific Research Projects coordination centers of Nigde and Selcuk University, Turkey

EPR study of gamma-irradiated N-hydroxysuccinimide

WOS: 000251837800010The electron paramagnetic resonance of gamma-irradiated single crystal of N-hydroxysuccinimide has been studied for different orientations of the crystals in a magnetic field. The radicals produced by gamma-irradiation have been investigated between 150 and 300 K. The spectra were found to be temperature independent and radiation damage centers were attributed to [GRAPHICS] radicals

EPR study of gamma-irradiated tetra-N-butylammonium iodide

WOS: 000300852100004The electron paramagnetic resonance of gamma-irradiated single crystal of tetra-N-butylammonium iodide has been studied for different orientations of the crystals in a magnetic field. The radical produced by gamma irradiation has been investigated at 300 K. The spectra were found to be magnetic field dependent and radiation damage centers were attributed to the (C alpha H2C beta H2C gamma H2CH3) radical. The principal values of the hyperfine coupling tensor of the unpaired electron with these atoms and the principal values of the gtensor were determined and the hyperfine coupling constants were verified by computer simulation. The results were found to be in good agreement with the existing literature data and theoretical predictions

Temozolomide and AZD3463 have synergistic anticancer effect on T98G glioblastoma multiforme cells

52nd Conference of the European-Society-of-Human-Genetics (ESHG) -- JUN 15-18, 2019 -- Gothenburg, SWEDENAvci, Cigir Biray/0000-0001-8251-4520WOS: 000489313904119[No abstract available]European Soc Human Gene

Comparative effect of imatinib and ponatinib on autophagy and miRNome in chronic myeloid leukemia

WOS: 000414115100021PubMed ID: 28942039BCR-ABL tyrosine kinase inhibitors (TKIs) are selective therapies for the patients with Chronic Myeloid Leukemia (CML). Imatinib and ponatinib have remarkable long-term efficacy on a major molecular response. Although TKI related induction of cytotoxicity and apoptosis have been clearly investigated in molecular levels, their comparative effect on autophagy and miRNome are largely unknown. This study aimed to investigate the involvement of alterations of miRNA expressions in CML progression, and how imatinib and ponatinib affect this process, by comparing CML, imatinib-resistant CML and leukemia stem cells (LSC). Cytotoxicity analysis was conducted by WST-1, apoptosis was evaluated by AnnexinV, autophagy was analyzed by Tb/GFP TR-FRET LC3B assay and changes in miRNomes were evaluated with microarray method. Ponatinib showed higher cytotoxicity and apoptosis at far fewer concentrations than imatinib. Both imatinib and ponatinib was able to trigger autophagy in imatinib-resistant K562ima3 cell line but not in LSC. We pointed that imatinib and ponatinib caused significant miRNA profile alterations, especially in the expressions of miR-214-pre, miR-218, miR-19a-5p, miR-19b-1-5p, miR-27b-pre, miR-23b-pre, miR-320e, miR-200a-pre, miR-508-3p, miR-33-pre and miR-766. This study is the first comparative miRNome analysis of CML, resistant CML and LSCs following the imatinib or ponatinib treatment and may guide to identify new markers for diagnosis, follow-up of the disease and to develop novel therapeutic strategies if supported by preclinical studies.Ege University Research Projects (APAK)Ege University [APAK 12-TIP-017]This study is supported by Ege University Research Projects (APAK) within the scope of the project numbered APAK 12-TIP-017

The effect of ICRT-3 on Wnt signaling pathway in head and neck cancer

WOS: 000450823500035PubMed ID: 30145828The effect of Wnt pathway in head and neck cancer could not be elucidated, even though the aberrant Wnt signaling plays a key role in the development of many types of cancer. The inhibitor of beta-catenin responsive transcription (ICRT-3) blocks the Wnt signaling pathway by binding to beta-catenin, which is a coactivator of the Wnt signaling pathway and a promising agent for inhibiting aberrant signaling. In our study, we aimed to evaluate the effect of ICRT-3 on the cytotoxicity, apoptosis, cell cycle progression, migration, and gene expressions in head and neck cancer stem cell (HNCSC) and hypopharynx cancer. The effect of this compound on cytotoxicity and cell viability in FaDu and HNCSC line was assessed by using the water-soluble tetrazolium salt-1 method. The effect of ICRT-3 on apoptosis was detected by using Annexin V and caspase-3, caspase-9 kit, on cell cycle progression by cycle test plus DNA reagent kit, on gene expression by dual luciferase reporter assay, and on migration activity by wound healing assay in both cell lines. ICRT-3 was determined to have cytotoxic and apoptotic effect in both cell lines. In addition, it was also found that the administration of ICRT-3 caused cell cycle arrest and significant decrease in gene expression level and migration ability of the cells.Ege University Scientific Research ProjectEge University [16-TIP-076]Ege University Scientific Research Project, Grant/Award Number: 16-TIP-07

Analysis of long non-coding RNA (lncRNA) expression in hepatitis B patients

WOS: 000433285000006PubMed ID: 29669510Long non-coding RNAs (lncRNAs) have been implicated in numerous biological processes, including epigenetic regulation, cell-cycle control, and transcriptional/translational regulation of gene expression. Differential expression of lncRNAs and disruption of the regulatory processes are recognized as critical steps in cancer development. The role of lncRNAs in hepatitis B virus (HBV) infection is not well understood. Here we analyzed the expression of 135 lncRNAs in plasma samples of 82 HBV patients (classified as chronic patients, inactive carriers, or resolved patients) at diagnosis and at 12 months of treatment in relation to control group (81 healthy volunteers). We also investigated the effect of small interfering RNA (siRNA)-mediated silencing of lincRNA-SFMBT2 on HBV-positive human liver cancer cell line. lncRNA expression was analyzed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Chemically synthesized siRNAs were transfected into the cell lines using Lipofectamine 2000 Reagent (Thermo Fisher Scientific). HBV DNA and HBsAg and HBeAg were detected in transfected cultures by real-time PCR and ELISA, respectively, using commercial kits. We observed changes in lncRNA expression in all three HBV groups, compared to control group. Most notably, the expression of anti-NOS2A, lincRNA-SFMBT2, and Zfhx2as was significantly increased and expression of Y5 lncRNA was decreased in chronic HBV patients. A decreased Y5 expression and increased lincRNA-SFMBT2 expression were observed in inactive HBsAg carriers. The expression of HOTTIP, MEG9, and PCAT-32 was increased in resolved HBV patients, and no significant change in the expression of Y5 was observed, compared to control group. siRNA-mediated inhibition of lincRNA-SFMBT2 decreased the level of HBV DNA in human liver cancer cells. Further research is needed to confirm the prognostic as well as therapeutic role of these lncRNAs in HBV patients.TUBITAK (the Scientific and Technological Research Council of Turkey) grantTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [215S225]This work was supported by TUBITAK (the Scientific and Technological Research Council of Turkey) grant (Project No: 215S225)